随机引物标记

PCR和随机引物标记探针的方法比较

The comparison between PCR and random primer labeled method

方法32P随机引物标记氯霉素耐药基因为探针，以SlotBlot、Southernblot法检测，同时以氯霉素乙酰转移酶活性（CAT）和药敏试验（MIC）作为耐药标志。

Methods Detection was carried out by slot blot and southern blot , using Cm resistance gene labeled with ~ ( 32 ) P by random primed method as the probe . CAT and MIC were exploited as the marker of drug resistance .

用地高辛随机引物标记的人巨噬细胞炎性蛋白1α和血管细胞粘附分子1cDNA探针与各组内皮细胞进行核酸原位杂交。

The EC of all groups were hybridized , insitu , with the digoxigenin-labeled MIP-1 α and VCAM-1 cDNA probes .

随机引物法标记放射同位素DNA探针及其应用

Random primer extension radiolabeled DNA probe and its application

地高辛随机引物法标记探针的Southern杂交技术优化

Technological Improvement of Southern Blot Using Digoxigenin-labeled Probes with Random-primed Method

PCR引物法及随机引物法标记cDNA探针杂交效率的研究

The Study on Hybridizing Efficiencies of cDNA Probes Labeled with PCR Priming and Random Priming

对麦类作物的基因组DNA，宜采用随机寡核苷酸引物标记法进行生物素标记，适当延长标记时间可以提高标记效率。

Whereas the random oligonucleotide labelling is more suitable for genomic DNA probe and the labelling efficiency could be increased by prolonging the labelling time appropriately .

方法：以随机引物法标记人perforincDNA探针，对慢性丙肝患者肝活检标本进行原位杂交，观察肝局部浸润炎性细胞perforinmRNA的表达及分布，分析他们与肝损伤之间的关系。

METHODS : The expression and distribution of perforin mRNA in the local infiltrated cells were detected by in situ hybridization using a dig labeled perforin cDNA probe in liver biopsy tissues .

结果与结论：Taq酶标记法和大肠杆菌Klenow片段随机引物延伸标记法同样有较好的标记效果，且随机引物或特定引物作为延伸引物均可以合成足够有效的探针。

Results and conclusion : Taq DNA Polymerase labeling method is as efficient as Klenow fragment random primer DNA labeling system .

以大肠杆菌Klenow片断随机引物延伸标记法作为对照。

However , the essential content of law has its own form which is the inner structural character . coli Klenow fragment random primer DNA labeling system served as control .

对双随机引物的RAPD标记的效果进行了分析。

The effects of double primers RAPD was analyzed .

利用随机引物PCR方法标记样品中的病毒靶序列，标记产物与基因微阵列上的探针杂交，清洗、扫描后进行结果分析。

The nucleic acids of the selected viruses were amplified and labeled by random-primer PCR method , and then were hybridized with the respiratory virus detection DNA microarray . The microarray were scanned after hybridization and washing , and the results were analyzed .

按随机引物法以地高辛标记HBVdna探针，与按缺口翻译法以~（32）P标记的探针进行了比较。

The whole HBV DNA sequences were labelled by random primer incorporation of Digoxigenin - labelled dUTP , and compared with ~ ( 32 ) P-labelled probe by nick translation .