附红细胞体

犬附红细胞体病PCR检测方法的建立及与应用

Establishment and Application of PCR Technique for Detecting Dog Eperythrozoon

猪附红细胞体人工感染及药物治疗初步研究

Preliminary study of artificial infection and drug treatment of pig Eperythrozoon

PCR技术在附红细胞体感染模型中的应用

Application of PCR Techniques in Detection of Eperythrozoon in Rat Infection Models

猪附红细胞体实时荧光定量PCR检测方法的建立

Establishment of a real-time fluorescent quantitative PCR assay for the detection of Eperythrozoon suis

半套式PCR方法检测猪附红细胞体病的建立

Establishment of Half-nested PCR Method on Eperythrozoon Diagnosis

单抗捕捉ELISA检测猪附红细胞体血清抗体方法的建立

A capture ELISA with McAb for detection of antibodies against Eperythrozoon suis

附红细胞体病瑞氏、姬姆萨、吖啶橙染色法及PCR检测法的比较研究

Comparison of staining method with Wright , Giemsa and acridine stains with PCR assay for the detection of Eperythrozoon

为进一步完善猪附红细胞体的血清学诊断技术，本研究应用提纯抗原建立了检测猪附红细胞体抗体的间接ELISA方法。

We also developed the indirect ELISA to detect E. suis antibodies for consummate the serodiagnosis methods .

附红细胞体套式PCR方法的建立及其在交叉感染研究中的初步应用

Establishment of a nested PCR assay for the detection of Eperythrozoon and its preliminary application on the study of cross infection

通过实验室染毒实验，建立感染猪附红细胞体病的小鼠模型，并应用研制的PCR方法进行实验室验证。

A mouse model was established through the toxicity test in the lab and the lab test was conducted using the PCR method .

附红细胞体可以感染SPF昆明小鼠。

The purified Eperythrozoon can infect SPF Kunming mice .

绵羊附红细胞体部分16SRRNA基因序列测定和系统进化分析

Sequencing and phylogenetic analysis of the partial 16S rRNA in Eperythrozoon ovis

奶牛温氏附红细胞体16SRRNA基因的克隆及系统进化分析

Cloning and phylogenetic analysis of 16S rRNA gene of E. wenyoni in cow

附红细胞体对仔猪Ea花环率和EAC花环率的影响

Effect of E.suis on the formation ratio of Ea-rosettes and EAC-lei in Piglets

结论本试验建立的半巢式PCR诊断方法具有特异、敏感、实用等特点，为猪附红细胞体的检测提供了一种可靠的诊断方法。

Compared with the conventional PCR , semi-nested PCR appeared to be more sensitive and more practicable , thus providing a reliable method to detect M.suis .

根据附红细胞体在离体后12h内保持增殖活性的特性，血样必须在采后12h内处理。

Blood sample must be treated within 12h after collect from the body .

特异性试验和敏感性试验表明，所建立的PCR方法与常见支原体、细菌及原虫无交叉反应，能检测奶牛附红细胞体最低DNA量为0.145fg。

The specific and sensitive tests showed that there was no cross reaction with commonly-seen bacteria , Mycoplasma and Protozoa . The smallest amount of detectable E. wenyoni DNA was 0.145 fg .

结果显示，随着附红细胞体感染强度的增加，血浆中相应的NO和MDA含量逐渐升高，SOD活力逐渐降低。

The results were as follows : with the enhancing of the infection , the content of NO and MDA increased , and the activity of SOD decreased .

目的比较瑞氏、姬姆萨、吖啶橙染色法以及PCR法对附红细胞体的检出率，探索比较可靠的检测附红细胞体的方法。

The detection rates of the staining methods with Wright , Giemsa and acridine stains were compared in order to search for a reliable method to detect the presence of Eperythrozoon .

1950年Splitter证实附红细胞体是引起猪黄疸性贫血的原因，命名为猪附红细胞体。

1950 Splitter confirmed cause of red blood cells of pigs is the cause of jaundice anemia , named Pig Eperythrozoon .

猪PCV-2和附红细胞体混合感染的实验报告

Report on the combined infection of PCV-2 and E.suis

猪附红细胞体PPA-ELISA检测方法的建立

Development of PPA - ELISA method for detection of Eperythrozoon suis

本试验对GenBank中报道的猪附红细胞体的基因序列进行同源性比较分析，选择猪附红细胞体的保守序列作为扩增区域。

The experiment analyzed the homology of gene sequence of Eperythrozoon suis what were reported in GenBank , to select its conserved sequence as amplification district . The homology between C.

猪附红细胞体病（Eperythrozoon）是由猪附红细胞体寄生于猪红细胞的表面、血浆及骨髓内，以贫血、黄疸、发热等为主要临床特征的人畜共患传染病。

Eperythrozoonosis is caused by Eperythrozoon suis which parasites in the surface of erythrocyte , plasma and bone marrow of animal and human . The main clinical features are anemia , jaundice and fever .

结果表明，FMMU白化豚鼠对人附红细胞体比普通花色豚鼠敏感。

The results are as follows : FMMU albino guinea pigs were more easily infected with Eperythrozoon than the pigment ones .

本论文记述对猪附红细胞体病（Eperythrozoonsuis；E.suis）病原学特性进行的研究。研究主要包括E。

The research on the aetiological characters of Eperythrozoon Suis ( E.suis ) mainly contained the characteristics of chromaticity , morphology , in vitro maintenance and the effect of drug treatment in the paper .

比较结果表明，附红细胞体病仔猪的Ea花环率和EAC花环率均明显低于健康仔猪，且差异极显著（P<0.01），表明仔猪患附红细胞体病后，机体的免疫水平明显下降。

The results showed that the formation ratio of Ea-rosettes and EAC-lei in eperythrozoonosis piglets were obviously lower than that in healthy ones , and the differences were significant ( P < 0.01 ) which indicated that the immunity level in piglets descended obviously after infected with E. suis .

对急性附红细胞体病患畜（猪、羊）红细胞免疫功能的研究显示，感染附红细胞体的病猪RBC-CR1花环率显著下降，RBC-IC花环率变化不明显。

Erythrocyte immune function was studied in animals ( pigs and sheep ) with acute eperythrozoonosis . The results showed that The animals infected with eperythrozoon in the experimental groups showed a significant decrease of RBC-CR1 rosette formation rate , and the likeness of RBC-IC rosette formation rate .

奶牛附红细胞体病三种检测方法的比较研究

The comparative study between three detection methods of eperythrozoonosis in cows

结论附红细胞体的宿主特异性可被打破。

Conclusion We can break the host 's specificity of Eperythrozoon .