家蚕微孢子虫

家蚕微孢子虫PCR检测的研究

Studies on PCR amplification of DNA of pebrine spore Nosema bombycis from silkworm , bombyx mori

家蚕微孢子虫（NosemaBombycis）表面蛋白提取法研究

Study on the extraction of surface protein of Nosema bombycis

重度感染家蚕微孢子虫的家蚕丝腺cDNA文库构建及EST测序分析

CDNA Library Construction and EST Analysis of Bombyx mori Silk Gland Heavily Infected by Nosema bombycis

家蚕微孢子虫cDNA文库的构建及部分EST同源性分析

Construction of Nosema bombycis ( N.b ) cDNA Library and Analysis of ESTs Sequence From Partial Clones

家蚕微孢子虫（NosemaBombycis）孢壁蛋白提取方法的优化研究

Extraction of Spore Wall Proteins of Nosema bombycis With Improved Methods

设计1对正、反向引物btubf/btubr，对家蚕微孢子虫（镇江株）基因组DNA的β-微管蛋白（beta-tubulin）基因进行扩增，得到部分片段。

A beta-tubulin DNA fragment of the Zhenjiang strain of Nosema bombycis was obtained by primers btubf / btubr .

从这55个基因中随机抽取4个进行Southern杂交实验，结果表明这些水平转移基因确实存在于家蚕微孢子虫的基因组上，而非外来污染序列。

We randomly chose four of them to carry out the Southern blot , and the result shows that these genes exist in the genome of N. bombycis rather than the contamination . 2 .

孢子大小比家蚕微孢子虫（NosemaBombycis）略小。

Compared to Nosema Bombycis , it has a slightly smaller spore size .

35℃高温对家蚕微孢子虫在BmN细胞中发育、增殖的抑制

Inhibition of 35 ℃ on the Development and Propagation of the Nosema bombycis in BmN Cells

用铬变素2R法对家蚕微孢子虫孢子染色的研究

Determination of Horseradish Peroxidase Based on Enzymatic Reaction Using Chromotrope 2R as Substrate Study on the Staining of Spores of Nosema bombycis by Chromotrope 2R Method

serpin基因拷贝与片段重复及转座元件的相关性分析表明，在家蚕微孢子虫11个serpin中，有6个基因与转座元件存在极显著相关性，有两对基因是片段重复产生的多拷贝。

The relative analysis between serpins and segmental duplications and transposable elements indicated that there are six out of eleven serpins have significant correlation to the TEs , and two pairs of serpins were produced by segmental duplication .

家蚕微孢子虫转宿主果蝇的研究初探

Primary Study on Nosema bombycis Infecting the Insect of Drosophila

家蚕微孢子虫N.bombycis分离纯化方法的优化

Optimization of the procedures of isolation and purification of Nosema bombycis in silkworm

家蚕微孢子虫细菌人工染色体文库的构建

Construction and Analysis of Bacterial Artificial Chromosome ( BAC ) Library of Nosema bombycis

家蚕微孢子虫单抗金银染色法检测技术的研究

Studies on the monoclonal antibody based immunogold-silver staining method for detecting spores of Nosema bombycis

因此，我们推测家蚕微孢子虫可能在入侵过程中会分泌类枯草杆菌蛋白酶，通过降解昆虫体壁帮助其入侵宿主细胞。

So we speculate that subtilisin-like protease in N. bombycis may participate in the process of invasion .

家蚕微孢子虫孢壁蛋白的研究有助于阐明微孢子虫的侵染机制，从而可以为微粒子病的防治提供作用靶标。

The research on spore wall proteins is helpful to explain infective mechanism and provide a tool to prevent pebrine .

截止目前，家蚕微孢子虫的检测经历了以下阶段：显微镜检、分子生物学检测、免疫学检测。

The history of N. bombycis detection experienced the following phases : microscopic examination , molecular biology detection , immunology detection .

随着家蚕微孢子虫基因组测序的完成，通过序列比对发现在家蚕微孢子虫中也存在类枯草杆菌蛋白酶。

With the completion of genome sequence project of Nosema bombycis , it was found that subtilisin-like protease ( SLP ) also exist in this species .

目前在家蚕微孢子虫中仅鉴定报道5个孢壁蛋白，因此有待于新的孢壁蛋白鉴定。

To date , just a total of five spore wall proteins have been characterized in Nosema bombycis , thus it is time for us to identify new ones .

家蚕病原性微孢子虫的超微结构研究

Studies on the Ultrastructure of Microsporidias Pathogenic to Silkworm Bombyx mori

家蚕3种微孢子虫孢子表面抗原特性的研究

A study on the spore surface antigen specificity of 3 Microsporidians of silkworm

家蚕病原性微孢子虫孢子表面蛋白的选择性分离与总蛋白比较分析

Selective separation of EXOSPORAL protein and comparative analysis of total Sporal proteins of Microsporidias Pathogenic to silkworm Bombyx mori

胎牛血清及热处理家蚕体液对家蚕微孢子虫体外增殖的影响

Effects of FBS and Inactived Silkworm Hemolymph on the Development and Multiplication of the Nosema Bombycis in vitro

本研究就是以快速检测家蚕微粒子病为实验目的，制备家蚕微孢子虫的单克隆抗体，并初步探讨抗体在家蚕微孢子虫的快速检测上的可能应用。

In this study , we prepared monoclonal antibodies ( MAbs ) against N. bombycis and investigated the application of this antibody to rapid detection of silkworm pebrine .

为促进用PCR法检测家蚕微粒子病的生产应用，对带毒蚕卵中的家蚕微孢子虫DNA提取方法进行了研究。

The method for extracting Nosema bombycis DNA from Bombyx mori eggs infected with Nosema bombycis was studied to approach the feasible application of detecting through PCR .