体外培养

隐孢子虫虫种鉴定及安氏隐孢子虫体外培养研究

Study on Identification of Cryptosporidium and in Vitro Culture of Cryptosporidium Andersoni

X期鸡胚简易体外培养方法的探索

Study on Simple Method of in Vitro Culture for X-period Chicken Embryo

通过结果分析可知：体外培养胚胎中的H2O2含量从2-细胞早期到2-细胞晚期之间是逐渐增加的，然而从2-细胞晚期以后又逐渐下降；

However , H_2O_2 levels decrease gradually after late 2-cell stage .

铝对体外培养人T、B淋巴细胞合成肿瘤坏死因子影响的研究

Effects of Aluminium on TNF_ α Levels Which Produced by Human T and B Lymph Cells in Vitro Cultivation Experiment

结果体外培养的人眼睫状体无色素上皮细胞中存在有ANPmRNA的表达。

Results ANP mRNA existed in human nonpigmented ciliary epithelial cells .

体外培养大鼠晶状体，Western印迹分析确定重组腺病毒感染大鼠晶状体后CAT表达峰值时间。

The expression time course of catalase gene in rat lens infected by recombinant adenovirus was determined by Western blotting .

尼古丁对体外培养人外周血单个核细胞分泌TNF的影响

Effect of nicotine on the secretion of TNF of human peripheral blood mononuclear cells in vitro

目的探讨雌、孕激素对体外培养人类子宫内膜腺上皮细胞降钙素（Ct）表达的影响及其意义。

Objective To explore the effect of estradiol and progesterone on the expression of calcitonin in human endometrial epithelial cells cultured in vitro .

HBsAg进入体外培养的人绒毛膜滋养层细胞的初步研究

HBsAg uptake into human trophoblasts cultured in vitro

目的研究酵母系统表达人亲肝素性促轴突生长因子（heparin-bindingneurite-promotingFactor，HBNF）的活性，并观察其对体外培养细胞的促轴突生长作用。

Objective To express recombinant human heparin binding neurite promoting factor ( hrHBNF ) with yeast expression system , and to observe its in vitro activity of stimulating neurites outgrowth .

溶脲脲原体对体外培养大鼠支持细胞分泌IL-1α和IL-6的影响

Effect of Ureaplasma Urealyticum Infection on the IL-1 α and IL-6 Secretion by Rat Sertoli Cells

目的研究不同浓度的碘对体外培养人甲状腺细胞合成DNA、蛋白质和甲状腺激素分泌的影响。

Objective To investigate the effect of different iodide concentration on the synthsis of DNA , protein and secretion of thyroid hormone in cultured human thyroid cell .

体外培养的大鼠Schwann细胞神经生长因子和脑源性神经营养因子的表达

Expression of nerve growth factor and brain-derived neurotrophic factor in cultured Schwann cells

体外培养脊髓神经元损伤后c-Jun蛋白表达

Expression of c-jun protein in injured cultured spinal cord neurons

SNP对体外培养的海马神经元bcl-2和bax基因表达的影响

SNP induces the apoptosis of cultured rat hippocampal neurons by changing the expression of Bcl-2 and Bax gene

目的：研究多氯联苯（PCB）对体外培养大鼠海马神经元细胞生长、存活的影响。

Objective : To evaluate the effect of Polychlorinated Biphenyls ( PCB ) in cultured hippocampal neuronic cells of rats .

体外培养与扩增对脐血CD34~+细胞及HLA抗原表达的影响

Effect of Cultivation and Expansion in Vitro on CD34 ~ + Cells and HLA Antigen Expression of Umbilical Cord Blood

分别从老年人和新生儿外周血中分离淋巴细胞，用形态观察、DNA断裂电泳图谱、流式细胞仪分析凋亡峰等手段检测其在体外培养过程中发生凋亡的情况。

Apoptosis of human peripheral blood lymphocytes taken from old and newborn donors was detected by morphology observation , DNA fragmentation electropherogram , and flow cytometry analysis of apoptosis peak .

体外培养786-0肾癌细胞株，流式细胞仪（FCM）分析肾癌细胞Fas表达及凋亡。

Fas expression and cell apoptosis in cultured 786-0 renal cell carcinoma cells were detected by the flow cytometry .

目的基于肝脏发育生物学进展，建立有效诱导胚胎干细胞（ESC）向肝细胞分化的体外培养体系。

Objective To induce ES cells to differentiate into hepatocytes .

目的观察渗滤法水蛭提取液对体外培养视网膜色素上皮细胞（RetinalPigmentEPithelium，RPE）增殖的影响。

OBJECTIVE To observe effect of hirudinoid extract by percolation leech on proliferation of retinal pigment epithelium ( RPE ) cultured .

体外培养人眼小梁细胞黏附分子CD（44s）的表达

Expression of CD_ ( 44s ) in Cultured Human Trabecular Meshwork Cells

2体外培养的兔骨骼肌SC在10代以内生长稳定，超过10代以后肌SC的融合能力和形成肌管的能力也随之下降。

SCs grew steadily within 10 passages , and their ability to fuse and form myotubes faded after 10 passages .

TNF琢对体外培养的人脂肪细胞葡萄糖摄取和IRS-1酪氨酸磷酸化的影响

Effect of TNF α on Glucose Uptake and IRS-1 Tyrosine Phosphorylation in Cultured Human Fat Cells

体外培养条件下毛囊bulge细胞分泌的β神经生长因子的检测

Detection of β - nerve growth factor secreted by hair follicle bulge cell cultured in vitro

细胞周期分析提示体外培养的RPE细胞保持正常的繁殖能力。

The analysis of cell cycle showed the cultured RPE cells keep normal ability of proliferation .

IL-1β对体外培养NIT-1β细胞胰岛素分泌的影响

Effect of IL-1 β on the Insulin Secretion of in vivo NIT-1 Cell Line

采用实时荧光定量PCR、westernblot及荧光免疫组织化学方法探查FBN-1mRNA和蛋白在体外培养的子宫肌瘤平滑肌细胞中的表达。

FBN-1 mRNA and protein expressions in LSMC and myometrium smooth muscle cells ( MSMC ) were examined by quantitative real time PCR , Western blot and Immunofluorescence staining .

抗龋IgY和IgY牙膏对体外培养细胞影响的形态学观察

Influence of anti - dental caries IgY and IgY toothpaste on morphology of cultured cells

目的：检测Ca（OH）2激动体外培养的人牙髓细胞内Ca2+的释放和钙释放通道。

AIM : To investigate intracellular calcium ion release by Ca ( OH ) 2 stimulus in human pulp cells in vitro and to detect a system of calcium channel .