类胚体
- 网络EBs;embryoid body,EB;Embryoid bodies
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细胞体外培养可以分化为成纤维样细胞、上皮样细胞和类胚体。
The EG cells can differentiate into fibroblast-like cells , epithelia-like cells and embryoid body .
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采用碱性磷酸酶染色、核型分析、类胚体实验对扩增细胞进行ESC鉴定。
Subsequently we identified the expanding cells by alkaline phosphatase staining , karyotype analysis and the formation of embryoid body which are typical characteristics of ESCs .
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方法:胚胎干细胞在无饲养细胞、无碱性成纤维细胞生长因子(bFGF)、悬浮条件下培养形成类胚体。
Meth-ods : Human embryonic stem cells were cultured in suspension without feeder layers and bFGF in medi-um .
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方法:首先培养用于迁移实验的细胞,即源于类胚体的nestin阳性前体细胞。
Methods : The first step was to prepare nestin-positive precursor cells for cell migration experiments .
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目的探讨类胚体中残留未分化胚胎干细胞(ESC)的特性。
Objective To explore the residual undifferentiated mouse embryonic stem cells ( ESCs ) in embryoid bodies .
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采用类胚体法(EB)的EB分化4-/4+程序和单层分化法研究不同的实验方法对胚胎干细胞神经分化的影响。
The neural differentiation process of mouse embryonic stem ( ES ) cell induced by embryoid differentiation and monolayer differentiation was investigated in this study .
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方法悬浮法培养类胚体14d,10%中性甲醛固定,常规石蜡包埋切片,取具有子宫内膜样组织结构的切片行雌激素受体(ER)免疫组织化学染色。
Methods ( 1 ) Embryoid bodies were cultured in suspension for 14 days , fixed in 10 % neutral-buffered formalin and embedded in paraffin . Estrogen receptor ( ER ) was detected by immunocytochemical staining .
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将小鼠5d龄ES-D3细胞源的类胚体(EBs)单细胞,按5×104/mL接种6孔细胞培养板,在DMEM基础培养基中使EBs单细胞贴壁培养。于第14!
The cells of 5-day embryoid bodies ( EBs ) derived from murine ES-D3 lineage were inoculated into each well of 6-well culture plate at 5 × 104 / mL in DMEM .
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方法先诱导mESCs形成类胚体(EB),进一步诱导其分化为成纤维细胞(mEB-dFE),以用作饲养细胞。
Methods mESCs were induced to form mouse embryoid bodies ( EB ), and then fibroblast-like cells were derived from further differentiated mEB ( mEB-dF ), which served as feeder cells .
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以上结果表明,诱导hAFS细胞向心肌细胞分化时,通过形成类胚体并采用条件培养液的诱导效果最好。
The conclusion showed that the induce effect of hAFS cells differentiation into cardiomyocytes through the formation of embryoid bodies under the conditional medium was advantageous over other induce conditions .
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人类胚胎干细胞体外分化形成包含多种细胞的类胚体
Human embryonic stem cells forming embryoid bodies comprising multiple types of cells
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类胚体中残留细胞分化全能性维持与外源性白血病抑制分化因子的关系
Relationship between pluripotency maintenance of residual embryonic stem cells and exogenous leukemia inhibitory factor
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类胚体中残留未分化胚胎干细胞的初步研究
Residual undifferentiated embryonic stem cells in embryoid bodies
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竹类果实胚体的比较解剖与系统分类
Comparative anatomy of bamboo embryo and their systematic classification