体外扩增
- 名in vitro amplification
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小鼠树突状细胞的体外扩增培养及形态观察
In vitro amplification and morphology of dendritic cells from mice
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核酸体外扩增技术的新发展
Development of in vitro amplification of nucleic acids
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DNA体外扩增结果良好。
The result of PCR for the DNA was good .
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DNA体外扩增技术&聚合酶链式反应(PCR)
DNA Amplified Technique Out body Polymerase chain Reaction ( PCR )
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结论双链引物PCR是一种特异性更好的DNA体外扩增技术。
Conclusion The double strand primers reported here may increase the specificity of PCR amplification .
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目的比较不同来源的树突状细胞(DC)在体外扩增、分化成熟的特点。
Objective To compare the growing behavior of dendritic cell ( DC ) from different sources .
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经α-半乳糖酰基鞘氨醇体外扩增Vα24自然杀伤T细胞的生物学特性
Ex vivo Expansion of V α 24 Natural Killer T Cells with α - galactosylceramide
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方法体外扩增培养、成骨诱导犬BMSCs。
Methods Isolated BMSCs were expanded in vitro and osteogenic induced .
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造血细胞生长因子对脐血CD(34)~+细胞短期体外扩增的作用
Effect of Hematopoietic Growth Factors on Short Expansion of Umbilical Cord Blood CD_ ( 34 ) ~ + Cells in vitro
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目的探讨核酸体外扩增(POLYMERASECHAINREACTION,PCR)技术在骨结核病诊断与鉴别诊断中的应用价值。
Aim : To evaluate the clinical value for polymerase chain reaction ( PCR ) technique in diagnosis and differentiating diagnosis of bone tuberculosis .
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目的观察造血抑制因子TNFα对人脐血基质细胞体外扩增的效果。
Objective To study the effects of TNF α on human umbilical cord blood stromal cell ( HUCBSC ) colony .
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结论采用添加IL-2培养体系是体外扩增Treg细胞的优选方法,其增殖效能强于IL-10;
Conclusion IL-2 is superior to IL-10 for Treg proliferation in vitro .
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小剂量X射线照后TIL细胞体外扩增及抗癌作用的变化
Reaction of low does ionizing radiation on amplification in vitro and antitumor effect of TIL cells
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乙肝病毒蛋白抗原表位特异性CTL体外扩增条件优化
Optimizing the conditions for in vitro expansion of HBV protein epitope-specific CTLs
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方法:利用PCR技术,以HBV病毒DNA为模板,体外扩增HBVPreS/S基因。
Method : HBV preS / S gene fragments were amplified from HBV DNA in vitro , using PCR techniques .
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MSC增加细胞因子的脐血细胞体外扩增的作用;
MSC enhance the effect of cytokines on cord blood cell expansion ex vivo .
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聚合酶链反应作为一种强有效的DNA体外扩增技术,已被广泛地应用于一些遗传性疾病的临床基因诊断。
Polymerase Chain Reaction ( PCR ) as an efficient DNA amplification technique in vitro hasbeen widely al (?) lied to clinical gene diagnosis of some hereditary diseases .
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研究结果如下:1、成功分离了大鼠BMSCs,并实现体外扩增培养。
BMSCs were isolated from rat marrow and culture successfully in vitro . 2 .
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本实验建立了用聚合酶链式反应(POLYMERASECHAINREACTION,PCR)技术体外扩增哺乳动物SRY特异序列鉴别哺乳动物胚胎性别的方法。
A Polymerase Chain Reaction ( PCR ) - based method for sex determination of mammals embryos by amplification of mammals SRY-specific sequence was established .
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人神经生长因子cDNA的克隆&人神经生长因子cDNA的体外扩增及纯化
Molecular Cloning of cDNA of Human Nerve Growth Factor : The Amplification in Vitro and Purification of cDNA of Human Nerve Growth Factor
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PHA对CIK细胞体外扩增影响的研究
Experimental study on effect of PHA on proliferation of CIK cells in vitro
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CIK细胞的体外扩增及其抗肿瘤特性的研究
Study of the expansion in vitro and its anti-tumor character on CIK cell
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抗人CD3单抗重、轻链可变区基因的体外扩增、克隆和序列分析
Amplification , Cloning and Sequence Analysis of the Genes of V_H and V_K of an Anti-human CD3 Monoclonal Antibody
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牛SRY序列的体外扩增
Cattle SRY - Specific Sequence Amplification
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GM-CSF对脐血CD34~+巨核祖细胞体外扩增及分化的影响
Effect of GM-CSF on Expansion and Differentiation of CD34 ~ + Megakaryocyte Progenitor Cells from Cord Blood in vitro
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结果成人骨髓MSC在体外扩增15代可获得(2~3)×1012个细胞。
Results Bone marrow MSC proliferated as undifferentiated cells in culture for 15 passages and ( 2-3 )× 10 12 cells were obtained .
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根据水稻的保守区设计引物,利用PCR体外扩增技术,从大米草中克隆Na+/H+逆转运蛋白基因片段。
A segment of Na ~ + / H ~ + antiporter gene from English cordgrass was cloned by PCR using a pair of primers designed according to the conserved domains of rice .
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目的体外扩增培养兔骨髓间充质干细胞(MSCs)。定向诱导MSCs为类Schwann细胞。
Purpose : To cultivate rabbit bone marrow mesenchymal stem cells ( MSCs ) in vitro and induce it to differentiate into cells with Schwann cell characteristics .
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ADSCs体外扩增和自我更新能力很强,传代培养易于获得大量有分化能力的细胞。
ADSCs have strong ability to self-renewal and mass culture accessible to a large number of cell differentiation in vitro .
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转FL、GM-CSF基因的人骨髓基质细胞促进脐血CD34~+细胞体外扩增
Effects of hFL and / or hGM-CSF gene transfected human bone marrow stromal cell lines on expansion of CD34 ~ + cells from cord blood