交联酶

交联酶晶体及其催化特性的研究

Study on the Catalytic Characters in Form of Cross-linked Enzyme Crystals

交联酶晶体的性能及其在有机合成中的应用

The Properties and Application of Cross-Linked Enzyme Crystals in Organic Synthesis

CandidaRugosa脂肪酶交联酶晶体稳定性研究

The Study of Stability in CrossLinked Enzyme Crystals of Candida Rugosa Lipase

本文主要是采用小麦B淀粉进行交联酶水解生产低DE值的麦芽糊精，用来替代脂肪，并将应用到蛋黄酱中，从而达到替代油脂的目的。

This article mainly used the wheat B starch crosslinking that was hydrolyzed by enzymes to produce low DE value maltodextrin , used to replace fats , and will be applied to the mayonnaise to instead of alternative oil .

目前对交联酶聚集体的研究主要集中在制备及其性能考察方面，但对交联酶聚集体粒径的调控及其对CLEAs性能的影响关注很少。

The studies of CLEAs were focused on its preparation and characterization , and little attention to the particle size with the regulation and its influence on the performance of CLEAs .

碱性蛋白酶交联酶晶体的制备及稳定性

The preparation and stability of alkaline protease in form of cross_linked enzyme crystals

无定形硒纳米粒子的交联酶模板法合成及其在生物传感器中的应用

Amorphous Selenium Nanoparticles : Synthesis in Horseradish Peroxidase Cross-linked and Application in Biosensor

尤其在化学修饰方面：交联酶结晶技术、多聚体共价结合以及表面修饰等成果显著。

Chemical modification as manifested in crosslinked enzyme crystal , covalent attachment of polymers and surface modification retain useful .

交联酶晶体在有机合成中非常有用，如在手性化合物拆分，肽合成，及碳-碳键的形成等方面有着比较成功的应用。

CLEC catalysts are extremely useful in organic synthesis , especially in the resolution of chiral compounds , peptides synthesis , and the formation of C - C bonds .

作为一类新型的无载体固定化酶，交联酶聚集体以其制备方法简单、单位体积酶活高以及稳定性好等受到广泛的关注。

As a new type of carrier-free immobilized enzyme , cross-linked enzyme aggregates ( CLEAs ) with characteristics of simple preparation , high activity per unit volume and good stability has been extensively used in many fields .

谷氨酰胺转胺酶（TGase）是一种通过催化酰基转移来促使蛋白质之间发生共价交联的酶，其特殊的催化反应特性，使它具有广阔的应用领域。

Transglutaminase ( TGase ) is a kind of transfer enzyme that can catalyze acyl transfer reaction . It can catalyze the covalent crossing-links between proteins . With such a special catalytic characteristics , TGase can be widely applied in many fields .

结果表明淀粉经交联、酶解处理后影响到颗粒表面及内部，但未影响到晶体结构，采用交联再微孔化的处理方法改善多孔淀粉的结构强度和吸附功能是可行的。

The result showed that crosslinked porous corn starch was feasible to improve the starch properties .

交联淀粉微球酶降解过程的FTIR和XRD分析

FTIR and XRD Analysis of the Enzymatic Degradation of Crosslinked Starch Microspheres

链霉菌谷氨酰胺转胺酶是目前唯一商业化的能够交联蛋白的酶制剂。

Streptomyces TGase is the only commercial enzyme that could covalent proteins .

分别采用鸡蛋膜固定化法，活化载体－共价结合法和戊二醛交联法进行酶的固定。

Using the activated fibroin as carrier , the papain was immobilized using covalence-crosslinking method .

用戊二醛简易法将辣根过氧化物酶与抗体蛋白交联，制成酶联免疫抗体。

Glutaric dialdehyde was used as cross-linking agent for coupling enzyme to pebrine spore antibody protein .

本文以戊二醛交联的辣根过氧化物酶（HRP）为模板合成了均匀分散的无定形硒纳米粒子（粒径10￣20nm），以所得合成产物为载体构建了HRP生物传感器。

Well-distributed amorphous selenium nanoparticles ( 10 ~ 20 nm ) were synthesized by using the horseradish peroxidase ( HRP ) cross-linked with glutaraldehyde as template . The selenium nanoparticles as-prepared have been used to construct HRP biosensor .

应用异型双功能交联剂制备酶-抗体结合物

Preparation of enzyme-antibody conjugate by heterobifunctional reagent SPDP

硝酸纤维素膜上化学药物交联抗原在斑点酶免疫法中的应用

Application of Antigen Linked with Chemicals on Nitrate Cellulose Membrane for Dot Enzyme Immunoassay

一种新的固定化酶方法&微粒明胶吸附交联青霉酰化酶

A novel method of immobilized enzyme & granular gelatin for adsorbing and crosslinking penicillin acylase

晚期糖基化交联产物裂解剂酶联免疫吸附测定筛选方法的建立

Screening breaker of advanced - glycation end - product cross - links by enzyme - linked immunosorbent assay method in vitro

研究者正在寻找解决这个问题的办法，包括血红蛋白交联所需要的酶，或者进一步改善血红蛋白的分子结构。

Researchers are studying ways to solve this problem , including cross-linking the required enzymes to hemoglobin or further modifying the molecular structure of hemoglobin .