个体基因组

结论大口鲇不同群体个体基因组DNA大小相同。

Conclusions Genomic DNA keeps the same molecular weight in different demes of Silurus meridionalis .

各种群罗氏沼虾个体基因组DNA的提取和纯化：采用SDS-蛋白酶K法进行提取，利用酚-氯仿-异戊醇进行纯化。

The purification of each individual 's gene DNA from the four populations : extract the DNA with SDS-proteinase K , then purify them with hydroxybenzene-chloroform-Isoamyl alcohol .

从40个寡聚核苷酸随机引物中筛选出17个扩增重复性好、条带清晰、特异性强的引物，对每个个体基因组DNA进行了扩增。

Of 40 random oligonucleotide primers for the amplification of amphioxus genomic DNA , 17 could produce reproducible , distinctive and characteristic fingerprints varying in length from 200 to 2 200 bp .

方法应用RAPD和AFLP两种方法对15只南江黄羊个体基因组DNA进行了分析，并比较两种方法的检测效果。

Methods The genomic DNAs of 15 goats were analyzed with RAPD and AFLP molecule marking techniques . The effects of the two methods were compared .

为了建立家蚕W染色体的特异性分子标记，采用RAPDPCR技术对同一品种家蚕雌雄个体基因组DNA进行分子标记筛选。

In order to set up the specific molecular marker of the Bombyx mori W chromosome , we adopted RAPD-PCR technology to screen the molecular marker from the same variety of male and female individual genome DNA of silkworm .

根据该片段碱基序列分别设计一对特异引物，以雌雄个体基因组DNA为模板进行PCR扩增，管家基因β-actin作为阳性对照。

According to the two fragments we have respectively designed a pair of primers , and did the PCR amplification with the template of male and female individuals genome , at the same time with the housekeeping gene β - actin as positive control .

用筛选出来的4种ISSR引物，对30只样本个体基因组DNA进行了ISSR扩增分析，得到962条扩增带，标记带为302条，特异带比率为31.4%。

Four ISSR primers were selected an amplified in ISSR analysis in genomic DNA of 30 JiLin White Goose in dividuals , and 302 specific bands ( 31.4 % ) distributed in 24 individuals were obtained .

对这个标记进行回收、克隆、测序，根据所得核苷酸序列设计了一对引物，仅能在雄性个体基因组DNA中扩增到到特异条带，成功的把AFLP标记转化为SCAR标记。

The marker was excised , cloned and sequenced . A pair of PCR primers was designed , which could amplify sex-specific bands in male genome DNA . So we converted the AFLP marker into a PCR marker , SCAR .

先到这里吧，希望我到周末有时间讨论NEJM第三篇文章，就其在个体基因组学的意义方面进行扩展。

That 's enough for now-hopefully I 'll have time over the weekend to discuss the third NEJM article , and expand on its implications for personal genomics .

哈佛医学院的大卫教授讨论了人类基因多样性和疾病的关系，乔治教授解译了个体基因组项目的复杂性。

HMS professor David Altshuler discusses the relationship between human genetic variation and disease , and HMS professor George Church explains the complexities of the Personal Genome Project .

在这篇文章中，我将对上述3篇文章中的两篇进行讨论，希望将来有时间处理第三篇，并在下一篇文章中论证这种辩论对个体基因组学未来的意义。

I 'll discuss two of these articles in this post , and will hopefully have time to tackle the third article-and the implications of this debate for the future of personal genomics-in a second post .

14个随机引物对28个个体的基因组DNA进行扩增，共得到143条DNA条带，其中多态性条带占96.5%。柞蚕品种内个体间的多态性为45.8%～49.4%；

The DNA polymorphism of different individuals within cultivar reached 45.8 % - 49.4 % .

第一批测序的个体人类基因组属于著名的遗传学家J。

The first sequenced individual human genomes belonged to famous geneticists J.

个体间基因组甲基化水平差异较大。

Methylation level varied bigger among individuals .