转录链

反转录聚合酶链反应（reverse-transcriptionpolymerasechainreaction，RT-PCR）扩增甲型流感病毒的全基因组或部分基因组序列并进行序列测定。

Whole-or part-genome viral sequences were amplified by reverse-transcription polymerase chain reaction and determined .

方法：采用乙醛酸溶液制作大鼠肾结石模型，采用反转录聚合酶链反应（RT-PCR）技术检测肾结石大鼠OsteopotinmRNA的表达。

Methods : The rat model of calculus of kidney was made by glyoxylic acid .

方法提取人外周血单个核细胞总RNA，反转录聚合酶链反应（RT-PCR）扩增目的基因；

Methods RNA from human peripheral blood mononuclear cells were extracted and target genes were amplified by RT-PCR .

目的建立实时荧光定量反转录聚合酶链反应（RT-PCR）法检测HLA-DRα基因mRNA表达。

Objective To establish a real-time quantitative RT-PCR method for detection of HLA-DR α mRNA expression .

方法：从人胎肝中分离总RNA，经反转录聚合酶链反应（RT-PCR）得到endostatin全基因。

Methods Human endostatin gene fragment was acquired from human fetal liver total RNA by means of RT PCR .

利用反转录聚合酶链反应（RT-PCR）技术在转录水平检测成骨细胞标记物骨桥蛋白（OPN）mRNA的表达。

The osteogenic marker osteopontin ( OPN ) mRNA were examined by reverse transcriptase polymerase chain reaction ( RT-PCR ) .

评价反转录多聚酶链反应检测大肠癌患者血清人端粒酶反转录酶mRNA表达的敏感性及特异性：对照组比较

Sensitivity and specificity of human telomerase reverse transcriptase mRNA expression in the serum of patients with colorectal carcinoma : a controlled comparison using reverse transcription polymerase chain reaction

目的：应用反转录聚合酶链反应（reversetranscriptasepolymerasechainreaction，RTPCR）法检测大肠癌的淋巴结微转移。

Objective : This study was undertaken to detect lymph node micrometastases in colorectal cancer patients by using reverse transcriptase polymerase chain reaction ( RT PCR ) .

半定量反转录聚合酶链反应和免疫印记法测定孕激素受体mRNA和蛋白质表达；

The expression of progestogen receptor ( PR ) mRNA and protein was measured by semi quantitive reverse transcription polymerase chain reaction ( RT PCR ) and Western blot .

于骨折后4,7,14,21d收集骨痂样本，反转录聚合酶链反应测定诱导型一氧化氮合酶的mRNA表达水平。

The specimens of bony calluses were collected at 4,7,14 , and 21 days after fracture to detect the level of iNOS mRNA expression by reverse transcription-polymerase chain reaction .

本研究建立了反转录聚合酶链反应（RTPCR）技术检测猪瘟病毒（HogCholeraVirus，HCV）方法。

In this study reverse transcription coupled with the polymerase chain reaction ( RT PCR ) has been established to detect hog cholera virus ( HCV ) .

反转录多聚酶链反应（RT-PCR）方法检测大鼠海马NPYmRNA的表达。

At the same time , The expression of neuropeptide Y ( NPY ) mRNA in hippocampus was determined by reverse transcription polymerase chain reaction ( RT-PCR ) .

对40份盲样采用琼脂扩散试验（AGP）、血凝抑制试验（HI）和反转录聚合酶链反应（RT-PCR）进行了检测。

40 blind samples were examined by agar gel precipitation ( AGP ), hemagglutination inhibitory ( HI ) test and reverse transcription-polymerase chain reaction ( RT-PCR ) .

方法采用荧光定量反转录聚合酶链反应（FQ-RT-PCR）方法检测40例患者鼻息肉组织中GR-αmRNA和GR-βmRNA的定量表达。

Methods The expression of GR - α / β mRNA in 40 samples of nasal polyp and 30 normal nasal mucosa was examined by using Fluorescent quantitative PCR .

结果如下：1.建立了适合提取玉米根和叶的总RNA的体系，并反转录成双链cDNA，完整性较好。

The results were as follow . 1 . Establishing the isolation technology system adaptable for total RNA in roots and leaves , and reverse transcription into double-stranded cDNA . Its integrality was fine . 2 .

用反转录聚合酶链反应方法（RT－PCR）及原位杂交方法（ISH）研究壁细胞生长抑素受体亚型的表达。

To identify SSR on parietal cells , positive SSR-specific primers by reverse transcription polymerase chain reaction ( RT-PCR ) and its probe by in the hybridization ( ISH ) were used .

在低氧和常氧培养后2,8,16,32h应用半定量反转录聚合酶链反应和蛋白质印迹技术检测其血管内皮生长因子基因mRNA和蛋白表达。

The expressions of vascular endothelial growth factor mRNA and protein were detected with semi-quantitative reverse transcription-polymerase chain reaction ( RT-PCR ) and Western blot at 2 , 8 , 16 and 32 hours after culture under hypoxia and normal oxygen respectively .

反转录聚合酶链反应（RT-PCR）检测P21cip1/WAF1mRNA、SURVIVINMRNA的表达。

The expression of p21 cip1 / WAF1 mRNA and survivin mRNA were analysed by reverse transcription polymerase chain reaction ( RT-PCR ) . Results The cell growth was inhibited significantly , with a time and dose dependent fashion .

连续给药8周后，用放射免疫法测定各组大鼠非梗死心肌血管紧张素Ⅱ及醛固酮表达水平，用反转录聚合酶链反应方法检测血管紧张素Ⅱ受体mRNA的表达。

After 8 weeks of continuous administration , the levels of angiotensin ⅱ and expression of aldosterone in non-infarction myocardium of rats in each group were measured with immunohistochemical method , and RT-PCR was adopted to detect the mRNA expression of angiotensin ⅱ receptor .

结果提示，反转录聚合酶链反应是测定CD36抗原基因表达的理想方法，可以用于动脉粥样硬化的研究。

Conclusion RT PCR is an ideal method to determine expression of CD36 antigen gene , which might be used in the study of atherosclerosis .

方法采用巢式反转录聚合酶链反应（RT-PCR）技术特异引物扩增细胞角蛋白19（CK19）mRNA，检测76例肺癌患者外周血及淋巴结中CK19mRNA的表达情况。

Methods Nest reverse transcription polymerase chain reaction ( RT-PCR ) was used to detect cytokeratin 19 mRNA ( CK19mRNA ) expression in peripheral blood and lymph nodes in patients with lung cancer .

利用反转录聚合酶链（RTPCR）技术，扩增出禽呼肠孤病毒（ARV）S1133、1733长为540bp的S1基因片段。

Using a reverse transcription polymerase chain reaction ( RT PCR ) the 540 bp long S1 gene of two strains ( S1133 、 1733 ) of avian Reovirus ( ARV ) were amplified successfully .

方法建立自体静脉移植模型，采用反转录聚合酶链反应（RTPCR）和免疫组织化学方法，对ECE、ET1和增殖细胞核抗原（proliferatingcellnuclearantigen，PCNA）在移植静脉的表达情况进行分析。

Methods A model of autogenous vein graft was established by interposition of the jugular vein into abdominal aorta in 80 Wistar rats . RT-PCR and immunohistochemistry were employed to test mRNA and protein level of ECE , ET-1 and proliferating cell nuclear antigen ( PCNA ) .

两种反转录聚合酶链反应检测甲型肝炎病毒

Detection of Hepatitis A Virus by Two Reverse Transcription PCR Approaches

反转录聚合酶链反应方法检测溶血磷脂酸3种受体亚型基因表达谱。

The mRNA expression of LPA receptor subtypes was determined by RT-PCR .

竞争性反转录聚合酶链反应对髌韧带中的α1-Ⅰ型胶原蛋白基因表达的定量检测

Detection of α 1-Type-I Collagen Gene Expression in Patellar Tendon Using Competitive Reverse Transcribed Polymerase Chain Reaction

分析发现甲基化位点处于中间载体部分，目标片断序列未出现甲基化，因此，该载体仍能在受体细胞中起到正常转录双链RNA.的作用。

It analysis showed that the target fragment sequence does not methylated , and methylation sites is media vector , therefore , the vector still in normal transcription of receptor cells play double-stranded RNA role . 3 .

未研究采用反转录多聚酶链反应技术观察了低氧对培养的大鼠大脑皮层微血管内皮细胞中诱导型一氧化氮合成酶基因表达的影响。

Present study observed the direct effects of hypoxia on expression of inducible nitric oxide synthetase ( iNOS ) in endothelial cells ( CMEC ) which isolated from rats'cerebral cortical microvessels , using reverse transcription polymerase chain reaction ( RTPCR ) technique .

套式反转录-聚合酶链反应检测恙螨及鼠肺内肾综合征出血热病毒RNA

Nested Reverse Transcription Polymerase Chain Reaction for Detection of Hemorrhagic Fever with Renal Syndrome Virus RNA

用反转录-聚合酶链反应检测B组轮状病毒应用反转录聚合酶链反应（RT-PCR）法。

Detection of Group B Rotavirus by RT PCR Methods : To employ reverse transcription-polymerase chain reaction ( RT-PCR ) .