插入片段

转化效率和AD质粒的插入片段的大小表明酵母文库的质量较理想。

The efficiency of the transformation and the size of the insert fragment satisfied the quality of the yeast library .

通过PCR鉴定阳性克隆子中cDNA插入片段的大小和酶切验证后测序。

Positive clones were identified by PCR in the cDNA insert size and restriction enzyme digestion verified and was sent to sequencing .

经酶切和测序对插入片段进行分析、鉴定。经限制性内切酶和DNA测序分析两个基因是否已经正确连接到真核表达载体中。

The sequence of fusion gene was examined by enzyme incision and DNA sequencing .

用定量PCR从杂交阳性融合噬菌斑中分离候选插入片段

Isolating candidate inserted fragment from positive fused phage clones using quantitative PCR

结果文库扩增后得到28个阳性克隆，进行菌落PCR分析，均得到200～1000bp插入片段。

Results The amplified library contained 28 positive clones .

随机挑选12个克隆，其中11个克隆PCR扩增出插入片段，插入效率约为91%。

The insertion was amplified in 11 of the 12 randomly selected clones by PCR .

PCR扩增阳性克隆插入片段。

The inserts were amplified by PCR .

并对cDNA插入片段进行序列测定。

The cDNA inserts in the positive clones were PCR amplified and sequenced .

结果共筛选出13个阳性克隆，PCR扩增出特异的插入片段。

Results Thirteen positive clones were obtained after three rounds of immunoscreening , and all amplified by PCR .

PCR结果表明，这些重组腺病毒DNA的插入片段大小是正确的。

DNA , respectively . The PCR results showed that the insertions of the recombinant adenoviruses were of the right size .

菌落PCR分析结果表明，95%左右的阳性克隆含有大小200～700bp的插入片段。

Colony PCR analysis showed that 95 % of the positive clones containing the size of 200 ~ 700 bp of the inserts .

结果随机挑取50个直径>1mm的白色克隆进行PCR扩增分析，其中47个克隆有插入片段，克隆阳性率为94%。

Results Random analysis of 50 white clones with PCR amplification showed that 47 clones contained inserted fragments . The positive rate was 94 % .

文库的滴度、重组率及插入片段的大小是鉴定cDNA文库质量的重要指标。

Titre , recombination rate and the size of the inserting fragment are important index for the quality of the cDNA library .

结果PCR产物琼脂糖电泳结果显示：在预期位置有阳性条带；序列分析和酶切结果证实插入片段序列正确。

Result A positive band was formed when product of PCR electrophresed on agar gel and inserted fragment was confirmed correctly by sequencing and enzyme digestion analysis .

抗冻肽cDNA插入片段经杂交证实后，对其进行了酶谱分析，核酸序列测定。

After confirmation of the insert segment of the cDNA of antifreeze protein gene , the zymogram was detected and the sequence of the nucleotides was determined .

重组子经EcoRⅠ酶切验证显示：重组质粒均含有外源DNA插入片段。

The results after digested by restriction enzyme EcoR ⅰ revealed that the recombinants which had been picked out contained foreign DNA fragments .

结果文库容量为1.9×106个重组子，文库中cDNA插入片段大小介于0.4×103～6.5×103bp。

Results There are 1.9 × 10 6 recombinants in this library . The cDNA fragments ranged between 0.4 × 10 3 bp and 6.5 × 10 3 bp .

进一步DNA测序结果显示插入片段与tsbp编码序列一致，阅读框架完整，并且无移码。

Further DNA sequencing manifested that the insertion element was tsbp sequence with complete reading frame and no frame shift .

其中减数分裂时期正向消减文库共获得276个重组菌落，经菌液PCR鉴定获得了229个阳性插入片段，片段大小均在100～1000bp之间。

276 recombinant clones were obtained from forward subtractive cDNA library of meiosis period , 229 of which were positive insertional fragments with the average sizes of 100 ~ 1000 bp by PCR identification .

对重组质粒的分析表明，插入片段的序列与发表的VDR基因编码序列相同。

The sequence of the insert in the plasmid was identical to the published coding-region sequence of VDR gene .

结果表明，以λ噬菌体DNA为模板直接测序比PCR产物经回收后为模板进行测序，其目标克隆的平均插入片段长度要长，但反应成功率低。

The results showed that the insert fragment length of target clones as λ phage DNA template directly sequencing was longer than that of PCR products after recycling , but a lower succeeding ratio for sequencing reaction .

联合蓝白斑和菌落PCR方法筛选含插入片段的阳性克隆，进行测序和同源性分析，并经Northern杂交技术检测基因的表达。

Clones with inserts were selected with combination of α - complementary phenomenon and colony PCR method . The sequencing and homology analysis were then performed and the mRNA expression levels were measured by Northern blotting .

随机选取50个克隆，用位于pRAC质粒多克隆位点两侧的测序引物进行菌落PCR扩增，检测文库插入片段的分布情况。

50 clones were selected randomly and the colony PCR amplification was used to detect the distribution of library inserts with pRAC sequencing primers .

方法：以弓形虫RH株速殖子感染大鼠血清作为探针筛选弓形虫cDNA文库，对阳性克隆的插入片段进行PCR扩增及DNA序列测定。

Methods Rats were infected with Toxoplasma gondii RH strain and their serum was used as a probe to screen Toxoplasma gondii tachyzoite cDNA expression libraries . The positive clones were analyzed by PCR amplification and DNA sequencing .

插入片段中存在一个RNA聚合酶Ⅲ启动子及AluⅠ内切酶识别位点，所以该片段可能为Alu成分。

According to the RNA polymerase ⅲ promoter structure and Alu ⅰ restriction enzyme site in the inserted fragment , it should be regarded as an Alu element .

对该文库特性研究表明，该文库包含了2.15×106个单个克隆，背景低于100pfu／μgDNA，插入片段平均大小约为17kb，证明是一个较为完整的脐橙基因组DNA文库。

A DNA library with 2 . 15 × 106 individual clones and a low background at 100 pfu / μ g DNA was obtained . The average length of inserts is about 17 kb . The library was proved to be standard and representative .

结果：构建了2×109大容量天然人源Fab抗体库，经核苷酸序列分析，证实插入片段为Fab基因片断。

Results : A large naive human Fab phage-display library consisting of 2 × 109 clones was successfully constructed . The sequencing result demonstrated that it was a human Fab gene .

质粒经NotI酶切鉴定插入片段大小。

Inserts size were identified by Plasmid NotI digestion .

在根据插入片段酶切指纹图谱对SSH文库中的414个阳性克隆进行分类的基础上，从12个出现频率较高的类型中各选一个克隆测序。

Based on the restriction enzyme-digested fingerprinting patterns , 414 positive SSH clones randomly picked from the library were classified into 73 groups , one clone from each of the 12 groups with the higher frequencies was sequenced .

对经EcoRⅠ酶切鉴定为阳性的重组表达质粒测序，结果显示插入片段大小、方向、碱基匹配与预期一致。

After sequencing the positive recombinant plasmid which was identified by EcoR ⅰ, results showed that the inserted fragment was right in length , direction and base matching .