插入载体

结果：PCR及序列分析显示融合基因插入载体正确；

Results : The gene insertion was proved correct using PCR and DNA sequencing .

抗体基因插入载体的频率，高比例的丢失意味着筛选的低效率；

The frequency of gene fusing , high deletion of antibody gene meaning low efficiency ;

方法设计特异引物，用RT-PCR方法从乳腺癌组织扩增获得目的片段，利用T/A克隆将PCR产物插入pMD-18T载体；

Methods The interested segment was obtained by reverse transcription PCR with the designed specific primers , and inserted into pMD-18T vector by T / A match .

将其插入表达载体pET-32a，获得了重组表达载体pET-32a-CD4。

Then the segment was inserted into vector pET-32a and reconstructed the recombinant vector , named pET-32a-CD4 .

双酶切及测序结果表明：目标基因已成功插入表达载体pET-32a（+）。

Restriction endonuclease digestion analysis and sequencing data revealed that the target gene has been successfully inserted the expression vector pET-32 a ( + ) .

利用PCR技术从猪伪狂犬病毒基因组中克隆gE抗原表位基因，并将其插入到载体pET-22b（+）中，构建成原核表达质粒pET-22b（+）-gE，使其在E。

By PCR , a gE antigen epitope gene was obtained from the genome of swine pseudorabies virus . We inserted the gene into an expression vector to yield the recombinant plasmid pET-22b ( + ) - gE .

方法：用DNA重组技术构建EGF基因并插入表达载体pQE-40，与载体上的6×His标签融合，获得的表达质粒pQE-EGF转化E。

Methods : An artificial egf gene was constructed with recombinant DNA technology and inserted into pQE-40 to get a expression plasmid pQE-EGF , in which egf was fused with 6 × His tag . Plasmid pQE-EGF was transformed into E.

方法用PCR的方法由含survivin基因的真核表达载体扩增所需目的片段，然后将该片段插入逆转录病毒载体pMIG。

Methods Survivin fragments were amplified from eukaryotic expression vectors by PCR , then cloned into pMIG retroviral vectors .

以往技术的局限是对BAC的修饰需要多步的克隆把目的序列插入到穿梭载体上。

One of the original limits of BAC modification technique required multiple cloning steps to target foreign sequences into the shuttle vector .

插入PGEMT载体，转化至大肠杆菌；

TR cDNA was inserted into pGEM-T vector , and then be transformed into Escherichia coli .

结果1.经琼脂糖凝胶电泳鉴定证实Gax基因成功插入到目的载体上，获得重组腺病毒。

The Gax gene was successful connection with the adenovirus vectors , which was examined by the agarose gel electrophoresis . 2 .

将CP基因插入原核表达载体pBAD/HisC中，转化E。

The CP gene was inserted into pBAD / His C vector and expressed in E.

结果酶切鉴定证实人IκBα基因cDNA已插入原核表达载体pET32a（+）。

Results Endonucleases digestion confirmed that I κ B α gene cDNA has been inserted into the prokaryotic expression vector pET-32a ( + ) .

用PCR法扩增了牛疱疹病毒Ⅰ型BarthaNu/67株gB基因，将其插入原核表达载体pBAD/TOPO中构建重组质粒pBAD-gB。

The gB gene fragment of bovine herpesvirus-1 Bartha Nu / 67 strain was amplified by PCR and inserted into prokaryotic expressing vector pBAD / TOPO .

目的克隆编码mdr1基因的启动子并插入荧光素酶报告基因载体。

Objective To clone the promoter of multi-drug resistance 1 gene and insert it into a luciferase reporter gene vector .

将目的基因片段插入原核表达载体pET-15b来构建表达质粒pET-15b/RgpAcd。

The correctly fragment was linked with and cloned into a prokaryotic expression vector pET-15b .

用RT-PCR的方法钓取端粒酶RNA基因的cDNA，并将其反向插入到逆转录病毒载体pLNCX上，构建hTR基因的反义表达质粒。

The cDNA of hTR ( human telomerase RNA ) gene was cloned by the means of RT-PCR and inserted into the reverse transcription virus vector ( PLNCX ) to construct the antisense expression plasmid of hTR gene .

从银屑病皮损表皮中提取总RNA，反转录成cDNA，常规方法将目的基因插入原核表达载体pGEX-4T-1和真核表达载体pCR3.1，表达和纯化相应蛋白和表位肽段。

Total RNA was isolated from epidermis of psoriasis patients and mRNA was reversely transcribed into cDNA . The epitope genes were inserted into prokaryote ( pGEX-4T-1 ) and eukaryote expression vector ( pCR3.1 ) .

将其全基因序列分三段先后插入到PUC19/18质粒载体中。

The gene sequences divided into three successively inserted into a plasmid vector , PUC19 / 18 .

病毒和多种聚合物材料曾被用作将治疗性基因插入患病细胞的载体。

Viruses and various polymer materials have been used in efforts to insert therapeutic genes into diseased cells .

结果重组质粒通过双酶切产生了0.78kb目的插入片段及4.68kb载体片段；

Results The recombinant plasmid cut by incision enzyme EcoR I and Kpn I overnight generated a 0.78 kb fragment and a 4.68 kb fragment in 1 % agarose gel electrophoretogram .