插入基因

并运用双酶切技术、SDS-PAGE电泳、westernblot（WB）及ELISA法分别对插入基因片段的正确性、表达产物的活性及特异性进行检测。

The accuracy of inserted gene and activity , specificity of HIV p24 proteins were detected by two enzymes digestion technology , SDS-PAGE , Western Blot ( WB ) and ELISA .

他们说存在“基因污染”的危险&插入基因片段的转基因作物通过授粉途径对类似的非目标作物产生影响。

They say there is risk of'gene pollution ' & where the inserted gene of GM plants affects non-target plants of similar kinds via pollination .

通过对突变株的基本特征以及PCR鉴定后，再进行插入基因定位。

Mutants were identified by basic characteristic of Salmonella pullorum , special PCR and gene location .

方法女性患者输血前后自身对照，采取静脉血样，用PCR技术进行TAGVHD插入基因片段及人Y染色体特异基因荧光检测。

Methods The insert gene region of TA-GVHD and specific gene region of the Y chromosome was detected using PCR technique .

coliTAP（106）后，获得pYX（40）对重组子的酶切图谱、原位杂交及DNA序列的分析表明，插入基因的方向、读框正确。

Coli TAP 106 . Analysis of restriction enzyme mapping , hybridizing and DNA sequencing shows that the gene direction and reading frame are correct .

结果：经限制性酶切分析，RT-PCR及PCR方法鉴定，插入基因大小、位置、方向正确。

RESULTS : According to the results of restriction digestion , reverse transcription-polymerase chain reaction ( RT-PCR ) and PCR , the length , position and orientation of inserted genes were correct .

结果26例患者中，有14例患者输血后出现Y染色体特异基因片段检测阴性而同时TAGVHD插入基因片段检测阳性的结果，发生率为53.8%。

Results Of 26 patients , positive insert gene region of TA-GVHD and negtive specific gene region of the Y chromosome were detected in 14 cases after transfusion . The incidence of TA-GVHD was 53.8 % .

对这109条旁邻序列的分析和定位结果表明：有26条序列插入基因的编码区，83条插入基因间的非编码区，有部分T-DNA的右边界序列丢失。

Analysis and location of the 109 flanking sequence showed that 26 of the flanking sequence insert the coding region of genes , 83 insert non-coding region between genes , and some of those lose the T-DNA right border .

Cre/loxP介导的伤寒杆菌染色体上插入基因的切除

Cre / loxP Mediated Excision of Inserted Gene Fragment from Salmonella typhi Genome

这一次，不是用近亲植物杂交法，而是插入基因。

This time , instead of breeding plants with their closest relatives , scientists are inserting genes .

结论获得的阳性克隆插入基因片段可能为潜在的日本血吸虫病疫苗分子，可能是多抗原、多表位在照射致弱尾蚴中起免疫保护作用。

Thus , insertion gene fragment of the positive clones obtained may be the potential candidate antigen for the development of vaccines for schistosomiasis in the future .

插入IL-2基因优化HBVdna疫苗的研究

Study of optimization HBV DNA vaccine by insertion of IL-2 gene

一些公司正使用基因剪刀技术来改变植物现在的DNA，而不是插入外来基因。

Some companies are using gene-editing techniques to change the plant 's existing DNA rather than insert foreign genes .

测序结果显示，提取的重组质粒中插入的基因片段全长及核苷酸序列与CD基因完全相同。

The full length and sequence of nucleotide acid of the inserted gene in extracted recombinant plasmid was completely identical to the CD gene .

将LacZ基因表达盒插入TK基因缺失转移载体，筛选TK基因缺失的GTPV-TY突变株。

LacZ gene was inserted into TK gene deleted transfer vector , the TK / LacZ + / GTPV-TY mutant was screened .

如果在这种复制子中插入外源基因，由于复制子RNA在细胞胞浆内大量扩增，就使得外源基因得到有效表达，因此，复制子RNA可作为表达外源基因的良好载体。

When the heterologous genes are inserted into the replicon , amplification of replicon RNA in the cytoplasm of cells makes the heterologous genes express effectively , as a consequence , the replicon RNA can be made the excellent vector for expression of heterologous genes .

科学家们所使用的“Sunup”木瓜含有随机插入的基因，使其能够对木瓜环斑病毒具有免疫作用，这种病毒降低了木瓜的产量和水果质量。

The'SunUp'papaya used by the scientists contains randomly inserted genes to give it immunity to the papaya ringspot virus , which reduces papaya yields and fruit quality .

CAV-2有四个早期转录区，其中E3区是病毒复制的非必需区，缺失或插入外源基因不会影响病毒的复制。

CAV-2 has four early replication area , among them E3 district is the non - essential district to replicate , it will not influence the replication of virus to lack or insert the foreign gene of other species .

将传染性胃肠炎病毒的基因组人工改造成为感染性cDNA的表达载体，此载体可在ORF3处插入外源基因，异源基因绿荧光蛋白可稳定、高效地表达。

The genome of the TGEV has been engineered as an expression vector of an infectious cDNA , The vector led to the efficient and stable expression of a heterologous gene , inserted in the site previously occupied by the nonessential ORFs 3a and 3b .

马传染性贫血病毒受体插入型基因的原核表达

Procaryotic Expression of Inserted Receptor Gene in Equine infectious anemia virus

对扩增产物测序确定插入位点基因。

The amplified production was sequenced and determined the gene of insertion site .

基因操作涉及插入外源基因或者改变原有基因的活性。

Genetic manipulation involves inserting foreign genes or modifying the activity of existing genes .

关于插入新基因对于土豆过敏原的效应的信息几乎没有。

Little information is available on the effects of inserting the new gene on potato allergen content .

插入猴子基因并不比插入果蝇基因更困难。

It is no harder to insert monkey genes than it is to insert fruit fly genes .

载体是供插入目的基因并将其导入宿主细胞内表达或复制的运载工具。

Vector is a transfer tool inserting interest gene to host cell in order to duplicate or expression .

插入的基因必须含有能让其在植物体中表达的启动子。

The gene inserted must contain a promoter which will allow it to be expressed in the plant .

结果：获得沙蚕纤溶活性蛋白的编码序列以及插入此基因序列的重组菌。

Results The encoding gene of fibrinolytic activity protein was obtained as well as the recombined Escherichia Coli containing the gene sequence .

插入外源基因和植物组织培养技术相结合可再生出具有新特征的纯一的植物群体。

Methods to insert foreign genes are coupled with the methods of plant tissue culture to regenerate identical populations of plants with novel characteristics .

首先用生物信息学方法对照已被测序的拟南芥基因组预测有转座子插入的基因，共发现2639个转座子和基因的结合体。

Firstly , we predicted the genes associated with TEs using the available sequenced Arabidopsis thaliana genomes by bioinformatics method and found 2639 gene - TE associations .

核对各载体的阅读框在插入目的基因序列后均无移码，说明原核表达载体构建成功。

It suggested that prokaryotic expression plasmids constructed successfully when we checked the reading frame of the plasmids without any frameshift after purpose gene order insertion . 6 .