连接反应

科学研究者一直认为烷基硫酯是自然化学连接反应发生的不可缺少的一部分。

Scientific research workers think that the alkyl thioester is an indispensable part in native chemical ligation .

本研究构建了大菱鲆扩增片段长度多态性（AFLP）分析体系，对DNA提取、双酶切反应、连接反应、预扩增反应、选择性扩增反应和银染等步骤进行了分析。

AFLP ( Amplified Fragment Length Polymorphism ) analysis system for turbot was established in this study , with the relative processes being presented , including DNA extraction , double enzymes digestion reaction , adapter ligation reaction , pre-amplification and selective amplification reactions , and argent dyeing .

用Ti3SiC2粉料连接反应烧结SiC陶瓷

Joining of reaction bonded SiC ceramic using Ti_3SiC_2 powder as filler

酶与活化纤维连接反应溶液的最适pH值为65～70；

Optimal value of pH in the reaction solution linking PGA on an activated fiber was 6.5 ~ 7.0 ;

结论：利用PCR、酶切、连接反应等基因克隆手段，可以构建pEBFP/FP6双表达质粒。

Conclusion : pEBFP / FP6 coexpression plasmid can be constructed by gene cloning methods , such as PCR , enzymolysis and coupled reaction .

在多肽固相合成中，分别采用酰氯法和活化酯法，研究了20种Fmoc-氨基酸与Wang树脂的连接反应。

The reaction of twenty kinds of Fmoc-AA-OH linked to Wang resin using methods of acyl chloride or active ester was studied in solid-phase peptide synthesis .

连接反应介导的等位基因特异性扩增-微流控芯片电泳法同时检测多个SNP位点

Simultaneous Detection of Multiplex SNPs by Adapter-ligation Mediated Allele-specific Amplification ( ALM-ASA ) on CE-chip Electrophoresis Device

TTHA与IgG连接反应的研究

Study of conjugation reaction of TTHA to IgG

通过连接反应介导的PCR（LM-PCR）方法，评价iNOS和NO水平变化对DNA双链断裂（DSB）的影响。

The influences of iNOS and NO changes on the double-strand DNA breaks ( DSB ) were identified using ligation-mediated PCR .

用T7噬菌体包装提取物包装连接反应产物，铺板测定包装效率和scFv基因插入率。

The ligation product was packaged with T7 packaging extract . Packaging efficiency and scFv gene inserting rate were detected after plaque assay .

保护氨基酸与羟基树脂的连接反应

Reaction of the protected amino acid linked to the hydroxy resin

无痕施陶丁格连接反应机理的理论研究

Theoretical Study on Reaction Mechanism of Traceless Staudinger Ligation

目的：探讨质粒重组体构建中，重组体连接反应的时间对转化与基因克隆结果的影响。

Objective : To study the influence of time on ligation in construction of recombinant plasmid .

否则，即使只有一个等位错配的基因，连接反应也不会发生。

Otherwise even when there is an allele mismatch between them , no ligation would take place .

叠氮化合物和膦硫酯的无痕施陶丁格连接反应是一种有效生成酰胺键的方法。

The traceless Staudinger ligation of azides and phosphines is an effective method to produce an amide bond .

蛋白质剪接是蛋白质内含肽介导的，一种在蛋白质水平上翻译后的加工过程，它由一系列分子内的剪切-连接反应组成。

Protein splicing , which is an intein mediated posttranslational processing , involves a series of intramolecular cleavage - ligation reactions .

这不是相信与否，需要在这里没有信仰，这是一个硬连接反应。

It is not something to believe in or not , no faith required here , it is a hard wired response .

其机理研究有助于进一步拓展核酸连接反应在疾病检测、基因载体及核酸修饰与分析等方面的应用。

The research of ligation mechanism will promote the application of ligase in disease detection , gene carrier , modification and analysis of nucleic acids .

自然化学连接反应在蛋白质化学全合成中得到了广泛地应用。该方法能够在温和的水溶液中高效地实现肽片段的连接，从而生成天然或者非天然的蛋白质。

Currently the most important method in the field of chemical synthesis of protein is native chemical ligation , which can efficiently condense peptide segments in aqueous solutions under mild conditions to generate natural as well as non-natural proteins .

反应层1与SiC形成了紧密的连接，反应层1/反应层2界面处各相相互咬合在一起。

The two reaction layers both bonded to each other and to SiC tightly .

众所周知，RIP协议对连接失败反应迟钝，这一点在这里得到了明确的论证。

RIP has generally been known to be slow to responding to connection failures , and this behavior is clearly demonstrated here .

由于PEG具有柔韧的长链而不会对链端连接的反应中心的活性产生影响，因此该反应在均相体系中进行，不仅反应条件温和，产率良好，而且反应还具有良好的选择性。

Due to the long and flexible chain of PEG , PEG supported substrates could maintain high reactivity so that the reactions involved could be carried out under mild conditions in good yields .

连接最适反应时间为10h；

At least 10 hours for ligation .

分析了连接界面化合物反应层的形成和长大机制。

The forming and growth mechanism of brazing interface is outlined .

这将确保数据源连接正确地反应分析内容。

This will ensure that the data source connections are correctly aligned with the analytic content .

与全局编录服务器的所有连接都没有反应，或连接数低于运行连接池所需的值。

All connections to the Global Catalog servers are unresponsive or there are fewer connections than are required to run the connection pool .

应用依赖随机化末端连接物聚合酶链反应技术研究重铬酸钾对大鼠肺p53基因DNA损伤

Applying randomized terminal linker-dependent PCR to detect the DNA damage of p53 gene in rat

SiC陶瓷连接工艺及焊料反应产物研究

Study on the Joining Process of SiC and the Reaction Products of Filler

Al2O3/Ti/Cu瞬间液相连接中的界面反应

Interfacial Reaction in Transient Liquid-phase Bonding of Al_2O_3 to Cu with Interlayer of Ti Foil

Ag-Cu-Ti连接SiC/SiC接头界面反应和界面结构

Interface Microstructure and Reaction of SiC / SiC Joints Brazed by Ag-Cu-Ti