组织培养法

利用组织培养法保存野生苎麻资源的研究

Study on the Preservation of Wild Species of Boehmeria by Tissue Culture

微量沙眼衣原体组织培养法的建立及临床应用

Establishment of method of micro tissue culture of Chlamydia trachomatis and clinical application

方法采用界面组织培养法建立SD乳鼠的中脑脑片长期培养体系。

Methods A long-term midbrain slice culture system of SD pup was established according to the interface tissue culture method .

组织培养法速繁AloeVariegata技术

Quick Propagation of Aloe Variegata by tissue Culture

结论此法与常规RTPCR法相比具有更简便，判断标准更客观的优点，可代替组织培养法检测甲肝减毒活疫苗滴度。

Conclusions The above method is more convenient and objective compared with routine RT-PCR , which can be used to measure the titer of HAV live attenuated vaccine .

将灭活HSV-2接种于小鼠阴道后，取感染急性期阴道拭子和潜伏期腰骶神经节以组织培养法分离病毒。

After intravaginal inoculation with inactivated HSV-2 , the vaginal swabs in the acute infection phase and lumbosacral spinal ganglia in the latent phase were taken from the animals for isolating virus .

方法：采用组织培养法，以原代大鼠大脑皮层神经细胞为材料，制备咖啡因、KCI及NMDA诱导的钙超载损伤模型。

Methods : Three injured models induced by caffeine , KCI , and NMDA , respectively , were used to assay the action of tanshinone in cultured primary cortex neurone of baby rats .

试验结果如下：（1）利用氨基酸平衡指数调整三种氨基酸（Met、Lys、Leu）比例设计不同氨基酸指数的试验组（共6个组），采用组织培养法体外培养乳腺组织15天。

Results were shown below : ( 1 ) The amino acids ratio were adjusted ( Met , Lys , Leu ) with Amino Acid Balance Index ( AABI ), to design the different groups . The mammary gland tissue were cultured 15 days in vitro .

用组织培养法保存软骨活性

Study on vital cartilage preservation using tissue culture method

利用组织培养法低温保存马铃薯材料

Cold storage of Potato Germplasm by tissue culture method

组织培养法繁殖银杏苗木试验

Breeding seedling of maidenhair tree using tissue culture method

组织培养法快速繁殖川乌头种苗

Rapid Propagation of Aconitum carmichaeli Seedling by Tissue Culture

氯霉素眼药水对角膜上皮细胞的毒性&组织培养法研究

The Toxicity of Chloramphenicol Eye Drops to Corneal

从天然物提取法、生物组织培养法和化学合成法三个方面综述了紫草色素的制备方法。

The preparation of the Radix Arnebiae pigment is reviewed from extraction of natural plant , biological organization culture and chemical synthesis .

用平板稀释法和组织培养法获得11株生物菌株，其中4种抗性菌株对该菌有很好的抑制作用。

It is evident that 4 resistant strains can better inhibit the mycelium growth from 11 ones got by the plate dilute method and the tissue culture method .

利用莴苣为受体材料，通过组织培养法，研究了各供体材料对莴苣化感作用的大小顺序为黄瓜>西瓜>辣椒>番茄>白菜。

The sequence of donor material allelopathy on lettuce was cucumber > watermelon > hot pepper > tomato > Chinese cabbage , which was studied by tissue culture method .

结果：倒置显微镜观察，悬浮细胞培养法A组和组织块培养法B组扩增的角膜缘上皮细胞均能在羊膜上单层生长。

Results : A monolayer of cultured corneal limbal epithelial cells was observed by inverted microscope in both A group and B group .

[结论]此法具有简便、灵敏、省时的优点，可代替常规组织培养ELISA法应用于甲肝减毒活疫苗感染性滴度检测中。

[ Conclusions ] This method is simple , sensitive and time - saving and may replace the cell culture ELISA for testing of liver attenuated HAV vaccine .

方法：制备AA大鼠模型，用组织块培养法培养AA大鼠滑膜细胞，并通过形态学和流式细胞仪（FCM）进行鉴定。

Methods : The synovial cells from AA rats were cultured with synovial tissue and identified by morphology and flow cytometer ( FCM ) .

方法NB患儿新鲜手术标本，采用组织块培养法、酶消化培养法建立原代细胞系；

Methods The neuroblastoma specimen was obtained from surgery and the primary cell lines were established by tissue mass culture and enzyme digestion culture respectively .

采用酶消化联合组织块培养法分离的胎鼠头盖骨成骨细胞体外连续培养最多达到13d，AKP和茜素红S染色阳性均表现阳性结果。

Osteoblast were isolated from fetal mouse cranial bone with method which based on combination of tissue block and mixed enzyme digestion .

愈伤组织悬浮培养法研究长喙田菁（Sesbaniarostrata）根、茎、叶的重金属抗性

An Investigation for Heavy Metal Tolerances of Root , Stem and Leaf of Sesbania rostrata by Using Callus Suspension Culture Method

方法：体外试验，采用组织细胞培养法，观察药物对柯萨奇（CVB3）、疱疹病毒（HSV-Ⅱ）的抑制作用。

Methods : In vitro test : the inhibition on CVB_3 and HSV - ⅱ was observed through cell cultivation method .

方法：应用组织块培养法培养成纤维细胞，用原位杂交检测胶原酶和TIMP-1的表达。

METHODS : Fibroblasts were cultured by tissue block culture method and the expression of MMP 1 and TIMP 1 was detected by in situ hybridization and quantitative analysis were used in this study .

结果：采用改良的DMEM培养基，用组织块培养法和胰酶消化法纯化培养的细胞，大肠癌细胞原代培养的成功率较高。

ResultsOther conditions were the same , improved DMEM medium , explant method and trypsinization purification approach were better than others , primary cell culture had a high achievement ratio .

方法取2例雌激素受体阳性的皮肤增生期草莓状血管瘤标本，采用组织块培养法培养HVEC。

Methods Two strawberry hemangiomas with positive estrogen receptor staining from 2 infants ( case 1 and 2 ) were cultured ( HVEC ) .

结果采用组织块培养法，2周后成肌细胞的密度可达107/ml，免疫细胞化学分析显示90%以上的细胞呈骨骼肌特异的肌细胞生成素（myogenin）抗体染色阳性。

Results After 2 weeks , the number of skeletal myoblasts reached 10 7 per millilitre . Immunocytochemical analysis showed that over 90 % cells were stained positive for myogenin , a factor specific for skeletal myocytes .

为了进一步研究雪里蕻和白菜SGR基因的功能，本研究构建了雪里蕻和白菜SGR基因的RNAi沉默载体，并采用了农杆菌介导的花序浸染法和组织培养转化法，进行了雪里蕻的遗传转化。

To further study the function of potherb mustard and cabbage SGR gene , We have constructed SGR gene silencing RNAi vector of potherb mustard and cabbage , and have carried out genetic transformation of Brassica juncea with floral-dip and tissue culture transformation by Agrobacterium-mediated .

方法用消化组织块培养法体外培养猪耳廓软骨细胞。

Methods Pig auricle chondrocyte was cultured in vitro by digestion .

胶原酶消化法比组织块培养法的效率更高。

Two enzyme method was better then tissue mass method .