麻疹病毒

2006年杭州市麻疹病毒分离株N和H序列分析

Analysis of nucleotide sequence of measles virus in Hangzhou in 2006

我国麻疹病毒流行株的H和N基因变异速率探讨

H and N Gene Variation Rate of Measles Virus Strain in China

麻疹病毒H基因的特异性逆转录-聚合酶链反应检测

Detection of Measles Virus Hemagglutinin Gene by RT PCR

麻疹病毒中国株与日本株的H蛋白及抗原性比较

Comparison of H Protein and Antigenicity of Chinese Measles Virus Strain with Japanese Strains

麻疹病毒H1和H2基因型快速鉴定方法的改进

Improvement of the Rapid Identification Method for H_1 and H_2 Genotype of Measles Virus

麻疹病毒血凝素基因H的点突变与血凝作用的转变

The Point Mutations in Hemagglutinin Gene of Measles Virus Are Responsible for Alteration in Hemadsorption

抑制麻疹病毒体外复制的效应性小干扰RNA的鉴定

Identification of Effective Small Interference RNA Inhibiting Measles Virus in vitro

麻疹病毒融合蛋白（F）和血凝素（HA）在痘苗病毒中的表达

Expression of fusion protein and hemagglutinin of measles virus in vaccinia virus

用RT-PCR方法直接检测麻疹病毒血凝糖蛋白（H）基因

Determination of measles virus hemagglutinin gene by RT-PCR

目的研究沪191（S191）麻疹病毒疫苗株的核蛋白（N）基因。

Objective This research is aimed to investigate the nucleoprotein ( N ) gene of S_191 measles vaccine strain .

减毒麻疹病毒在体内外对HBV的影响

Effects of attenuated measles virus on hepatitis B virus in vivo and in vitro

12所民工子弟小学麻疹病毒IgG抗体总阳性率为45.83%。

The total positive rate of antibody IgG was 45 . 83 % .

ELISA法检测麻疹病毒IgM抗体血清外对照的设置及意义

Value of extra serum control in ELISA for measles virus IgM antibody detection

不同性别及不同年龄猴麻疹病毒抗体阳性率没有显著性差异（P＞0.01）。

The difference of the positive rate of measles virus to antibody among the different age monkeys was not remarkable ( P > 0 . 01 ) .

麻疹病毒血凝素蛋白（hemagglutininprotein，H）是介导麻疹病毒吸附、侵入宿主细胞的关键分子。

Measles virus Hemagglutinin protein ( H ) is the key molecule mediating MV infection in host cells .

目的观察减毒麻疹病毒在体内外对乙型肝炎病毒（HBV）的影响。

Objective To observe the effects of attenuated measles virus on hepatitis B virus replication inside and outside the body .

大肠杆菌LPS和麻疹病毒特异性抗体定量测定法的建立和在免疫缺陷病患者中的初步应用

Development of a quantitative immunoassay for E.coli and measles antigens and its application in patients with immunodeficiency

麻疹病毒IgG抗体总阳性率为45.83%，麻疹的免疫屏障已不存在易造成校内麻疹暴发流行。

45 . 83 % of positive rate of measles antibody IgG suggests the potential of measles outbreak in elementary school .

8株麻疹病毒均为H1基因型。

All of the isolated viruses were H1 genotype of measles virus .

由于此HA蛋白是原核表达产物，回避了真核表达系统复杂的操作过程和昂贵的费用，所以，这个麻疹病毒血凝素基因工程抗原有望成为一种新型、便捷的麻疹病毒诊断试剂

The results showed that the MV hemagglutinin gene engineering antigen might provide a more efficient diagnostic reagent for detection of the MV infection

在培养2215细胞的上清液中加入麻疹病毒，HBsAg和HBeAg的分泌均受到抑制。

When adding the attenuated measles virus in suspension of 2.2.15 cell , the HBsAg and HBeAg decreased .

经分子生物学检测初步显示目前流行的麻疹病毒株为H1基因型。

Molecular biology demonstrated that the predominate strain was the H_1 Measles virus .

河北省CDC共分离到3株麻疹病毒，均为H1基因型。

The 3 strains of wild measles virus isolated from Hebei Province are H_1 gene type .

结论：FQ-PCR法为麻疹病毒核酸检测提供了一种可靠的方法。

Conclusion : This FQ-PCR provides a more efficient method for measles virus RNA detection .

结果9株6种基因型麻疹病毒RTPCR均呈现阳性条带，说明本研究采用的RTPCR方法敏感。

All the 6 genotypes ( 9 strains ) of measles virus showed DNA positive strand , it means this RT-PCR method is sensitive for measles viruses .

通过聚合酶链反应对一组24个使用过的床旁检测测试条上的唾液标本进行检测，分析麻疹病毒血凝素（）和核蛋白（N）基因是否可以直接检出。

A panel of24 oral fluids was used to investigate if measles virus haemagglutinin ( H ) and nucleocapsid ( N ) genes could be amplified by polymerase chain reaction directly from used POCT strips .

而非麻疹病毒RTPCR结果为阴性，均未见阳性条带，说明该RTPCR方法特异。

While the RT-PCR result of all the non-measles viruses showed negative DNA strand , it means the RT-PCR method is special for measles viruses , not others .

用RT-PCR方法，从2005年6省分离的48株麻疹病毒株中扩增出核蛋白（nucleoprotein，N）基因C末端676个核苷酸片段。

The 676 nucleotide sequence of the C terminus of nucleoprotein ( N ) gene of these isolates were amplified by RT-PCR , and the amplicons were directly sequenced .

此次分离到的麻疹病毒为H1基因型，和中国的本土优势流行株相符。

The isolated wild-type measles virus is H_1 , the same geno-type as Chinese native prevailing measles virus .

结果9株麻疹病毒分离阳性株的RTPCR-RFLP基因分型结果与核酸序列分析结果完全一致，均为H1基因型。

The genotyping results for measles wild viruses by RT-PCR-RFLP were the same with that of DNA sequence : H_1 genotype .