限制性酶切位点

利用PCR方法，从含有3G1scFv基因的重组质粒中扩增出抗体基因，并使基因两端携带合适的限制性酶切位点。

3Gl scFv gene fragment was amplified and proper restriction enzyme sites were added at each end by PCR .

方法82例汉族女性雄激素过多症患者及14名健康女性进行ACTH兴奋试验，并应用PCR扩增产生限制性酶切位点方法检测已知的9个21-OHD常见突变位点。

Methods Eighty-two androgen excess cases and 14 healthy women underwent ACTH stimulating test during the follicular phase .

牛疱疹病毒Ⅳ型（DN-599株）DNA的限制性酶切位点图及重复序列分析

Physical mapping of bovine herpesvirus type 4 ( dn-599 strain ) DNA and analysis of its repetitive sequences

通过引物设计，在抗脯氨酸反馈抑制耐盐突变菌株9315114的proBA基因重叠区引入一个限制性酶切位点，分别扩增出proB和proA基因，并构建融合的proBA基因。

A restriction enzyme site was inserted in the overlapping region of proB and proA genes from a salt-tolerant mutant of B. subtilis 93151 , and a fusion gene was constructed by cloning proB and proA genes respectively .

并在15个限制性酶切位点上也存在差异；

And there were differences in15 restrict enzyme sites within whole ITS1 sequence .

中国钩端螺旋体rRNA基因限制性内切酶酶切位点多态性分类研究

Identification of Leptospira in China by mapped restriction sites polymorphisms of rRNA gene

PCR-RFLP（？）电泳结果显示由于突变的存在，使突变基因产生了新的限制性内切酶酶切位点，突变组基因被切为大小不同的两个片段，凝胶电泳图上出现新的条带。

The results of PCR-RFLP electrophoresis showed that the mutant gene produces a new restriction enzyme cutting sites because of the presence of mutations , so mutation gene was cut into two fragments of different size , new bands appeared on gel electrophoresis map .

方法提取40例哮喘和36名健康人外周血DNA，PCR扩增免疫球蛋白E高亲和受体β链基因，分析第二个内含子内限制性内切酶Rsa1酶切位点的多态性。

Methods Blood DNA samples were extracted from 40 asthmatic patients and 36 health subjects . After gene amplification by PCR , polymorphism of intron 2 Rsa 1 site in the gene of high affinity receptor β chain for IgE was analyzed .