阅读框架

DNA芯片分析HBV阅读框架多点基因突变的研究

Gene chip analysis of mutation of HBV-DNA open reading frame

其cDNA开放阅读框架编码1765个氨基酸。

Its cDNA open reading frame encodes 1765 amino acids .

DNA序列测定显示所得克隆的开放阅读框架长843bp。

The detection of DNA sequence showed that the length of the obtained cloning open-reading frame was 843 bp .

以该文库DNA为模板，运用PCR方法扩增出360bp的NGF和616bp的CNTF阅读框架片段。

The cDNA of NGF and CNTF was amplified by the method of PCR .

测序结果表明，该毒株的HA基因全长为1745bp，含有完整的阅读框架，编码566个氨基酸；

The HA gene was 1 745 bp in length and coded for 566 amino acids .

经序列分析，此cDNA为一个567bp长的开放阅读框架，编码由188个氨基酸组成的病毒外壳蛋白。

The cDNA was 567 bp in length with open reading frame of 188 amino acids .

K562细胞未知基因KH的开放阅读框架在大肠杆菌中的表达

The expression of open reading frame of unknown KH gene from K562 cells in E.coli

基因测序结果表明，该cDNA具有独立的开放阅读框架，编码1个由344个氨基酸残基构成的可溶性蛋白分子，属于免疫球蛋白超家族成员。

DNA sequencing analysis showed that the cDNA had an open reading frame encoding 344 amino acid residues without transmembrane domain and belonged to immunoglobulin superfamily .

进一步DNA测序结果显示插入片段与tsbp编码序列一致，阅读框架完整，并且无移码。

Further DNA sequencing manifested that the insertion element was tsbp sequence with complete reading frame and no frame shift .

未知基因KH开放阅读框架真核表达载体的构建及其对K562细胞增殖的影响

Construction of eukaryotic expression vector of open reading frame of unknown KH gene and its effect on proliferation of K562 cell

其HN基因开放性阅读框架（ORF）为1716bp，编码571个氨基酸；

Its HN gene has an opening reading fragment ( ORF ) of 1,716 bp and it encodes 571 amino acids .

测序结果表明所扩增的cDNA含有完整的开放阅读框架（ORF），有1410个核苷酸，编码470个氨基酸。在www。

The sequence indicates that the cDNA amplified by RT-PCR has a complete Open Reading Frame ( ORF ) with 1410 nucleotide acids encoding 470 amino acids .

结果表明：2毒株HN基因开放性阅读框架（ORF）均为1716nt；

The results showed that : strain YN-C1 and YN-C2 all have 1 716 nt opening reading fragment ( ORF ) length ;

方法通过基因芯片技术，将HBVdna进行PCR扩增，掺入荧光分子标记，与点阵列的寡核苷酸杂交，通过计算机分析，检测HBVdna的基因序列，观察HBVdna阅读框架各位点的自然变异。

Methods By using gene chip technique , HBV-DNA was amplified by PCR , incorporated with fluorescence , hybridized with oligonucleotide to detect the gene sequence of DNA by computer analysis and observe the natural mutation of HBV-DNA ORF .

开放阅读框架（ORF）453bp，共编码150个氨基酸。

Bp 3 ' - untranslated region and 453 ? bp open reading frame ( ORF ), which encodes 150 amino acids .

所有克隆到的gp120基因都具有完整的阅读框架，无大的缺失和插入。

All the Cloned gp120 genes have the complete open reading frames , and no major deletion and insertion .

结合位置权重矩阵（PWM，positionweightmatrix）和开放阅读框架（ORF，Openreadingframe）的长度分布特征建立了简单的方法识别翻译起始位点，此方法能很好地区分上游AUG和TIS。

Based on the position weight matrix ( PWM ) and length distribution of open reading frame ( ORF ), a simple method for predicting translation initiation sites is presented . It can identify TIS from upstream AUGs easily .

目的：构建K562细胞未知基因KH的开放阅读框架（openreadingflame，ORF）的原核表达体系。

Objective : To construct the prokaryotic expression system of open reading frame of KH gene ( KH-ORF ), an unknown gene in K562 cells .

它在进化上具有高度保守性，是一组不编码蛋白质，不具有开放阅读框架（ORF）的短序列RNA。

It is highly conserved in evolution , which is a set of encoded protein that does not have a short sequence of RNA open reading frame ( ORF ) .

提出了一个预测DNA序列中无内含子的开阅读框架（ORF）的理论方法&终止密码预测法；

A method for predicting first kind of open reading frame ( ORF ) is given , which is called Terminal Codon Method . The first kind of ORFs is the ORF that has not intron in it .

目的构建未知基因KH的开放阅读框架（KHORF）的真核表达体系，初步探讨未知基因KH对细胞增殖的影响。

Objective To construct an eukaryotic expression vector of open reading frame of unknown KH gene ( KH ORF ), and investigate its effect on cell proliferation .

比较2种阅读框架，以保守序列作为模板扩增得到完整的编码EIAV反式激活蛋白的基因。

The whole sequence of tat gene has been obtained by further polymerase chain reaction during which more conservative sequence was used as template .

结果限制性核酸酶切及序列分析表明重组表达质粒包含Pokemon基因编码区，阅读框架无移位；

Results Restriction enzyme digestion and DNA sequencing suggested that the recombinant expression plasmid contained correct encoding region of Pokemon , without reading frame shift .

序列分析的结果表明，此3.1kb外源片段中存在一长度为1347bp的完整的开放阅读框架（ORF），起始密码子为ATG，终止密码子为TGA，共编码448个氨基酸。

Sequence analysis revealed that the open reading frame of mcr gene was 1 347 bp in size , encoding 448 amino acids with ATG as initiation codon and TGA as termination codon .

真核基因起始与终止密码子旁侧序列的特征对于确定cDNA开放阅读框架（ORF）和预测基因组序列中的编码区（CDS）非常重要。

The characters of sequence flanking the start codon and stop codon in eukaryotic genes play an important role in defining the open reading frame ( ORF ) in cDNA sequences and coding region ( CDS ) in genomic DNA sequences .

ERIC（IRU）局限于基因组可转录区，即多顺反子操纵子基因间区域，或开放阅读框架上、下游非翻译区。

ERIC ( IRU ) is restricted to transcribed regions of the genome , either in intergenic regions of polycistronic operons or in untranslated regions upstream or downstream of open reading frames ( ORF ) .

小鹅瘟病毒GPV-YG株主要开放性阅读框架核苷酸序列分析

Analysis of the Major Open Reading Frames ' Nucleotide Sequence of Goose Parvovirus GPV-YG Strain Isolated in China

PTI基因翻译起点上游具有‘TATA’和‘CCAAT’等转录控制元件，其包含的最大阅读框架能编码一个213aa的多肽。

The longest open reading frame in the PTI gene , which contains conventional ' TATA ' and ' CCAAT ' transcription control elements , potentially encode a peptide of 213 amino acids .

测序结果表明该680bp片段含一个开放阅读框架，编码204个氨基酸，其中N端17个氨基酸为信号肽，中间43个氨基酸为Hevein成熟肽，C端144氨基酸为未知功能多肽。

The sequence analysis indicated that the fragment had a complete open reading frame that encoded a polypeptide of 204 amino acids with the N-terminal region containing 17 amino acids signal peptide , the mature hevein containing 43 amino acids and the C-terminal region containing 144 amino acids polypeptide .

利用载体pET-22b（+），构建chiB表达载体pET-chiB，通过验证分析表明，所克隆的基因chiB已置于表达载体的正确阅读框架下；

Utilizing vector pET-22b ( + ) to construct expression vector pET - chiB , through validating and analyzing , the result indicated that the cloned chiB gene had been inserted the correct reading frame of expression vector ;