无细胞系统

无细胞系统原位制备蛋白质芯片及其应用

In situ Fabrication and Application of Protein Microarray With Cell-free System

无细胞系统中染色质形成核状结构的研究

Study on Formation of Nuclear-like Structure around Chromatin in the Cell-free System

靛Ⅲ抑制无细胞系统DNA合成随剂量的增加而加强；

The inhibition of Dian ⅲ on DNA synthesis in free cell system increased with the increase of DNA polymerase concentration .

人胚肾培养细胞poly（A）~+RNA在兔网织红细胞无细胞系统中的转译

Translation activities of poly ( a ) ~ + rna from human embryo kidney cells in rabbit reticulocyte lysate system

检查睾丸细胞及其无细胞系统在NFZ作用后的DNA梯状电泳图谱以及与凋亡相关的Bcl-2、caspase-3等因子表达。

Examine the DNA electrophoretic profile of testicular cells after induction with NFZ as well as the expressions of Bcl-2 and caspase-3 , using cell-free system as control .

Vif基因体外表达载体构建及其在无细胞系统中的表达

Cloning and Expression of the HIV Protein Vif in Escherichia coli Cell - free System

微紫青霉CBHⅠ基因（Cbh1）在体外无细胞系统中的表达

In vitro Expression of CBH ⅰ Gene ( Cbh1 ) of Penicillium janthinellum in Coupled Transcription-Translation System

染色质在非洲爪蟾卵的无细胞系统中形成组装核的研究

The study on the chromatin forming assembled - nuclei in cell - free system of Xenopus eggs

最后在家蚕丝腺无细胞系统中探索了利用家蚕丝腺蛋白质启动子在体外合成蛋白质的可能性。

The native promoters of silk gland were studied for protein synthesis in this silk gland cell-free system .

爪蟾卵无细胞系统中核的自组装和组装核染色质纤维的研究

A Study of Nuclear Self-assembly and the Chromation Fibre of the Assembled Nuclei in the Cell-free System of Xenopus Eggs

近期有研究证明，吸入H2或口服富氢水在心、脑和肝缺血再灌注损伤后有保护作用，在体外无细胞化学系统中H2可以特异性的中和？OH及ONOO-，减少OS。

Recently some studies have shown that H2 inhalation or hydrogen water consumption exerted protective roles in heart , brain and liver after ischemia-reperfusion injury . In a cell-free chemical system in vitro , H2 selectively reacted with ? OH and ONOO - which eventually reduced the OS .

一种新的PrP无细胞构象转化系统的建立

Establishment of a New Cell Free Conversion System for Prion

用体外无细胞RNA合成系统对阿克拉霉素B（AcM&B）和阿霉素（ADM）抑制个同DNA模板的依赖DNA的RNA合成作了研究。

The inhibitory effect of aclacinomycin B ( AcM-B ) and adriamycin ( ADM ) on DNA-dependent RNA synthesis directed by different synthetic DNA templates has been studied using non-cell RNA synthesis system in vitro .

无细胞蛋白质合成系统是一种以外源mRNA或DNA为模板，通过在细胞抽提物的酶系中补充底物和能源物质来合成蛋白质的体外系统。

The cell free protein synthesis system enables the direct in vitro synthesis of protein from template DNA or mRNA by using the existing enzymes in the cell extract , which is supplemented with substrates and energy sources .

实验表明，可以利用原核系统取代真核系统表达PrP，并用生物素标记取代35S标记纯化蛋白，建立一个更加安全方便的无细胞构象转化系统，用于朊病毒的研究。

Our study supplies a new system , using more efficiently prokaryotic expressed PrP instead of that expressed in mammalian or insect cells and more easily handling and environmental protecting biotin instead of isotope in cell free conversion for prion study .

一种简单快速微量的无细胞蛋白合成系统

A Rapid and Simplified Microassay for Measurement of Protein Synthesis in Rabbit Reticulocyte Lysates

无细胞蛋白质合成系统

Cell - free Protein Synthesis System

该文介绍了无细胞蛋白质合成系统的技术进展及表达控制，并综述了这一系统的应用发展。

Here we report the most advanced technology for cell-free protein synthesis system and its application progress .

无细胞蛋白质合成系统相比传统的细胞表达系统有许多优点，包括表达周期短、反应条件容易控制等。

Cell-free protein synthesis has some advantages on protein expression and regulation , compared with traditional cell-based expression system .

本文以提高重组蛋白在大肠杆菌和无细胞蛋白合成系统中的可溶表达量为目的。

The purpose of the present work is to increase the expression level of soluble protein both in E. coli and in cell-free protein biosynthesis system .

无细胞蛋白质合成系统由于蛋白质合成能力强、操作快速简单、适于高通量表达各种蛋白质受到广泛关注。

Cell-Free protein synthesis system has attracted extensive attention due to its high ability of protein expression , simplicity of operation and the potential for high-throughput protein synthesis .

这是首次用克隆化DNA为模板，以植物RNA聚合酶B建立起的无细胞离体转录系统。

This is the first established cell-free system for in vitro transcription of cloned DNA with a plant RNA polymerase B.

本文以重组质粒pZmS51DNA为转录模板，在无细胞离体转录系统中研究了质粒DNA的构型变化与其模板活性的关系。

The relationship between template activity and structural variation of recombinant plasmid DNA was studied in a cell-free system for in vitro transcription which was developed with maize RNA polymerase B and recombinant plasmid DNA .