质粒抽提

用去内毒素的质粒抽提试剂盒提取重组质粒pXL/EGⅣ/GFP/Neo后，转染家蚕BmN细胞，确认GFP和Neo的表达正常。

The recombinant plasmid pXL / EG ⅳ / GFP / Neo , which is extracted using a endotoxin-free plasmid extracting kit , was used to transfect silkworm BmN cells , and the result showed that the GFP and Neo were expressed successfully .

丙烯酰胺降解细菌质粒抽提方法的比较

A Comparison of the Extraction Methods of Plasmid from Acrylamide Degrading Bacteria

碱变性加溶菌酶法对标本进行质粒抽提。

Plasmids were extracted by alkali with lysozyme .

几种细菌质粒抽提方法的研究

Bacterial Plasmid Extraction Methods for Several Bacteria

鸡蛋经二氯甲烷提取，用固相萃取柱净化处理后上机检测。碱变性加溶菌酶法对标本进行质粒抽提。

The egg samples were extracted with dichloromethane . Plasmids were extracted by alkali with lysozyme .

方法应用碱裂解法对淋球菌的质粒进行抽提，获得淋球菌临床分离株的质粒谱。

Methods The plasmid profiles were obtained by using alkaline-lysis plasmid extraction method .

金黄色葡萄球菌抗生素耐药质粒DNA的抽提及检测

Extraction and detection of antibiotic resistant plasmid DNA in Staphylococus aureus

通过转化感受态大肠杆菌，挑选阳性克隆，提取重组质粒，并对抽提的质粒进行限制性酶切反应，DNA测序鉴定。

Recombinant vectors were then transformed to E-coli , The positive colony were selected and recombinant plasmids were extracted . After restricted enzyme digestion and sequencing , the plasmids were identified correctly .

质粒的转化、抽提、纯化。

Routine methods were used in the transformation , extraction and purification of the plasmid pIRES-EGFP .

结果：①质粒鉴定结果：质粒抽提纯化后电泳可见3条DNA条带，符合质粒的电泳图谱；

RESULTS : ① Identified results of plasmid : After the purification , the electrophoresis of plasmid showed that there were 3 DNA straps , which was accorded with the electrophoretogram of plasmid .