碱裂解法

碱裂解法提取重组质粒DNA及PCR验证

Using Alkaline Lysis Method for Recombine Plasmid DNA Extraction and PCR Verification

目的对碱裂解法提取质粒DNA中主要试剂的作用进行研究。

Objective to explore the action of main reagent alkaline lysis method of extracting plasmid DNA .

一种适合芽孢杆菌质粒DNA提取的改良碱裂解法

An improved alkaline lysis adaptive to extracting plasmid DNA of Bacillus

碱裂解法提取质粒DNA的研究

The Compare Study on the Method of Extracting Plasmid DNA

改良的碱裂解法提取动物细胞附加体DNA

A modified alkaline lyses protocol for extracting episomal DNA from the mammalian cells

碱裂解法提取基因组DNA；PfHT1基因的PCR扩增、克隆；

Extraction of genomic DNA from FCC1 / HN using the alkali specific cleavage method ;

采用碱裂解法与DEAE-纤维柱层析法提取与纯化抑制素质粒DNA，用分光光度法测定其浓度和纯度。

The inhibin plasmid DNA was extracted and purified by alkaline lysis method and DEAE-fiber column chromatography and its concn .

用碱裂解法提取Ⅺ-11菌的质粒DNA，在0.7%的琼脂糖凝胶电泳上可检测到三条较大的质粒条带。

Three large plasmids were isolated and detected in strain XI-11 by using alkali lysis extraction and agarose gel electrophoresis .

方法：碱裂解法提取纯化质粒DNA，微量注射法直接将质粒DNA导入体内肿瘤；

Methods Alkaline lysis assay was used to extract and purify the plasmid . Plasmid DNA was injected into tumor by microinjection assay .

方法：快速碱裂解法提取线粒体DNA，长时程PCR扩增出5430bp线粒体DNA。

Methods : Mitochondrial DNA was isolated by alkaline extraction , the 5 430 bp fragment of mtDNA was amplified by a long PCR .

本试验采用碱裂解法提取模板DNA，这种方法比试剂盒提取方法操作简便，而且经济，快速。

This assay adopted alkaline analysis to extract DNA . The operation of this method is simple and convenient , and it is economic and fast .

实验运用改良的SDS碱裂解法对30株镰刀菌基因组DNA进行了抽提，并对rDNAITS区域进行了特异性扩增。

Thirty genomic DNA of Fusarium were extracted with improved SDS alkaline lysis method and the ITS district of rDNA was especially amplified .

以6个菊花品种为试材，利用碱裂解法、SDS法、CTAB法、改良SDS法提取DNA后，根据外观、琼脂糖凝胶电泳检测、A260/A280比值的测定、DNA产量等。

The total genomic DNA extraction from 6 chrysanthemum cultivars were reported based on base cleavage method 、 SDS method . CTAB method and improvement SDS method .

样品以80%酸化预冷甲醇提取，使用碱裂解法分析检测ABA结合态。

Samples were extracted with the 80 % precooling and acidified methanol and conjugated abscisic acid in extracts were treated by alkaline lysis method .

对表型阳性菌株，用碱裂解法提取质粒，聚合酶链反应（PCR）方法检测质粒的酶基因型别，检测较常见的SHV、PER及OXA2型，对阳性株进行测序。

The drug resistant gene of plasmids in positive strains were detected by PCR and the PCR products of SHV , PER , and OXA-2 type were sequenced subsequently .

结论碱裂解法提取HBV-DNA简单、快速，适用于HBV-DNA荧光定量测定。

Conclusion The alkaline lysis method is simple , rapid and efficient for extraction of serum HBV-DNA and suitable for HBV-DNA quantitative PCR detection .

方法使用替代试剂，建立改良的碱裂解法进行质粒DNA提取，并用琼脂糖电泳验证所得的质粒的质量，紫外测量估算产量及纯度。限制性酶切验证其用度。

[ Method ] Use alternative reagent to establish modified alkaline lysis method for extraction of plasmid DNA and use agarose electrophoresis to examine the quality of plasmid DNA , ultraviolet spectrophotometry to evaluate its production and purity , restricted enzyme cutting to examine its availability .

随机挑选单菌落小规模培养，碱裂解法提取质粒DNA，PstⅠ／BglⅡ双酶切后行变性聚丙烯酰胺凝胶电泳和银染分析，筛选含有不同等位基因的重组质粒后测序。

Use alkaline lysis method to extract the plasmid DNA . DNA constructs were identified by Pst I / Bgl II digestion , denaturing polyacrylamide gel electrophoresis and DNA sequence analysis .

本研究利用碱裂解法抽提RA临床分离株野生型质粒，通过酶切分析，引物延伸的方法获得质粒的全序列，并对其进行生物信息学分析，为质粒改造奠定基础。

Using the experiment of digestion single restriction endonuclease and the primer walking method , we obtained the complete sequence of the wild type plasmid . The bioinformatics analysis of the complete sequence laid a foundation for the reconstruction of the plasmid .

方法：采用纸片扩散法检测80株淋病奈瑟菌对7种抗生素的敏感性；碱裂解法分析产青霉素酶的淋病奈瑟菌（PPNG）株及耐四环素的淋病奈瑟菌（TRNG）株的质粒图谱；

Methods : Susceptibility to 7 antibiotic agents was tested by the disc diffusion method in 80 isolates , and plasmid DNA was obtained by alkaline lysis method in PPNG and TRNG strains .

碱裂解法反复抽提均未得到质粒，多次接合试验未成功。

No plasmid was extracted and conjugation was not successful .

方法应用碱裂解法对淋球菌的质粒进行抽提，获得淋球菌临床分离株的质粒谱。

Methods The plasmid profiles were obtained by using alkaline-lysis plasmid extraction method .

应用碱裂解法提取相应菌株的质粒，对质粒谱进行分型。

The plasmids were extracted by alkaline lysis technique .

方法：（1）采用碱裂解法提取淋病奈瑟菌质粒；

Methods : ( 1 ) The plasmids of N.

最后用碱裂解法裂解细胞提取质粒，并将提取物进行阴离子交换层析以进一步纯化质粒。

Finally , alkaline lysis and anion exchange chromatography were used to release and purify plasmids .