碱提酸沉法

采用碱提酸沉法研究了制取花生分离蛋白的优化条件。

Alkaline extraction and acid precipitation were employed to produce peanut protein isolate .

结果表明：①超声波提取法>索氏提取法>碱提酸沉法；

Whose results indicated that : ① The extraction rate by supersonic > traditional Soxhletextraction > alkali extraction and acid precipitation .

与传统的碱提酸沉法相比，红松仁分离蛋白的提取率可明显增加。

With the traditional " acid alkaline extraction " method , Korean pine nut protein isolate can significantly increase the extraction rate . 2 .

对两种方法进行比较，结果表明：两种方法提取大豆低聚糖的提取率基本相同，但醇法浸提大豆低聚糖的浓度明显高于碱提酸沉法。

The two methods are compared . The results show that the oligosaccharides extract-ratio of the two methods are almost the same , but the concentration of oligosaccharides extracted by the ethanol method is higher than the other one .

5种碱水（碱提酸沉法）提取比较，001%碱水提取的内酯含量（2151%）最高，所用成本最低，含水量（075%）最低，平均回收率为9688%。

In the five sodium carbonate solutions ( ie Sodium extraction and acid precipitation ), 0.01 % sodium carbonate solution had the highest lactone content ( 21.51 % ) with the lowest cost . The moisture content was the lowest . Its average recovery rate was 96.88 % .

结果表明，碱提酸沉膜分离法的分离效果明显优于传统的方法，所得的7S和11S大豆球蛋白的纯度可以分别达到75.5%和84.7%。

Results showed that the new method is better than traditional methods and the purity of7S and11S is75.5 % and84.7 % respectively .

本研究选择低温脱脂大豆粕为原料，提出并采用碱提酸沉膜分离法来分离7S和11S大豆球蛋白，通过SDS-PAGE电泳鉴定分离纯度，并与传统的几种方法比较分离效果。

This paper studied on the separation of 7S and 11S globulin from the defatted soy flake by a new method , which used the alkaline extraction , acidic precipitation and membrane technology . The purity of separation were determined and compared with some traditional methods by SDS-PAGE gradient electrophoresis .