胎牛血清

2.30%胎牛血清浓度培养基的应用明显提高人脐血MSCs培养的成功率。

30 % fetal calf serum concentration medium markedly improved the application of human umbilical cord blood MSCs culture success rate .

第二试验组的培养液是CMRL-1066加上胎牛血清（FCS）；

The medium of the second group was CMRL-1066 with fetal calf serum ( FCS ) .

在含12%以上胎牛血清的DMEM中细胞生长良好；

Cells grow well in DMEM that contain 10 % fetal cattle serum .

本实验以兔为模型，初步探索了利用异体血清替代胎牛血清培养MSC的方法和效果。

In this paper , we have established a rabbit model to use allogeneic serum for the culture of MSCs .

结果：原代软骨细胞在0.4%胎牛血清培养基培养条件下，不同浓度的bFGF和胰岛素对软骨细胞的增殖有剂量相关性。

RESULTS : bFGF and insulin exerted their related action on primary cartilage cells in 0.4 % fatal bovine serum at different concentrations .

我们发现在自体血浆培养条件下与在胎牛血清和人AB血清条件下培养得到的DC表型和功能相比，并无显著性差别。

We have found that there was no significant difference between autologous plasma and FBS or human AB serum culture condition on DC phenotype and function . 3 .

用胎牛血清诱导分化后，荧光显微镜下观察分化细胞的形态和GFP表达情况。

With fetal bovine serum induced differentiation , the differentiated cells were observed under fluorescence microscope morphology and GFP expression .

Beagle犬血清与胎牛血清生化特性及其对体外培养的人肺癌细胞生长的影响

Biochemical Analysis of Beagle Serum and Fetal Bovine Serum and the Biological Effect on Lung Carcinoma Cell Line in Vitro

培养基由DMEM、10%胎牛血清、1%双联抗生素配制。

The culture medium was compounded by DMEM , 10 % fetal bovine serum and1 % double antibiotics .

方法：在体外干细胞培养中，A组加入10%胎牛血清，B组加入10%小牛血清，C组加入10%马血清。

Method : During process of cell culture in vitro , 10 % fetal bovine serum was mixed into culture medium of A group , 10 % calf serum into B group , 10 % horse serum into C group .

方法取3-4dSD乳鼠坐骨神经，纯化培养许旺细胞，A组加入含10%胎牛血清的DMEM/F12培养基，B组加入自体神经匀浆激活的巨噬细胞条件培养基；

Method To take sciatic nerves in 3-4 days SD rats and purify Schwann cells . A group : DMEM / F12 contained 10 % calf blood serum . B group : conditioned medium of macrophages activated by self-neural homogenate .

本研究采用Fos蛋白免疫组化染色方法观察了胎牛血清对原代培养鸭肾上皮细胞c&fos表达的刺激作用及褪黑素的阻断作用。

In the present study the influence of melatonin on the expression c-fos in primarily cul-tured kidney epithelial cells of duck was studied by immunocytochemistry .

所获骨髓种植于细胞培养板内。培养液为含20%胎牛血清DMEM液。

All received are planted in the bone marrow cell culture plates , Mediums are DMEM containing 20 % fetal bovine serum solution .

胎牛血清诱导INPC/EGFP分化，观察分化后细胞的形态及EGFP表达。

After INPC / EGFP was induced by fetal bovine serum , the cell morphology and the expression of EGFP were observed in the differentiated cells .

实验首先用原位灌注法获得小鼠肝细胞、树鼩肝细胞，继而在含10%的胎牛血清的DMEM细胞培养基中建立相关细胞培养体系，细胞贴壁效率高，生长状况良好。

Then cells were cultured in DMEM medium with 10 % fetal bovine serum , with the result of high plating efficiency and excellent function maintenance .

在培养条件方面选取DMEM／F12培养液+20%胎牛血清培养猪耳皮肤成纤维细胞，效果较好。

The culture results of the porcine ear skin fibroblasts was good by using DMEM / F12 containing 20 % fetal bovine serum .

从3.5-4天的鸡胚中分离PGC，在添加5%胎牛血清和10ng/ml的LIF的DMEM培养液中进行原代培养24小时。

PGCs were isolated from the genital ridge of 3.5-4 day embryos and cultured in DMEM supplemented with 5 % FCS and 10 ng / ml LIF .

结果以选用4～24h贴壁的有核细胞，加入5%～10%胎牛血清、种植密度（4～8）×104个/ml的培养条件为最适宜细胞生长；

Results The best culture condition in vitro for growth was 4-24 hours adherent time , 5 % - 10 % fetal bovine serum , ( 4-8 )× 10 4 / ml cell density .

方法于含10%胎牛血清的DMEM培养基、37℃、含5%CO2的细胞培养箱中密闭培养口腔鳞状细胞癌细胞系SAS。

Methods The high metastasis adenoid cystic carcinoma cell lines SAS were contained in DMEM medium containing 10 % fetal bovine serum , 37 ℃, 5 % CO2 incubator in the closed-culture .

采用含30%胎牛血清的DMEM培养液以及使用95%O2和5%CO2的气体环境进行动脉血管的器官培养。

The artery organ culture was carried out in DMEM culture solution contain 30 % fetal bovine serum and gaseous environment of 95 % O2 and 5 % CO2 .

研究方法：（1）分离新西兰幼兔骨髓基质细胞，在含有10%胎牛血清、维生素C、β&磷酸甘油和地塞米松的DMEM培养液中，体外培养及诱导形成成骨细胞。

Methods : 1 , Bone marrow cells obtained from New Zealand rabbits , were cultured and differentiated into osteoblasts in medium ( DMEM ) supplemented with 10 % fetal bovine serum , ascorbic acid , beta-glycerophosphate , and dexamethasone .

Milli-Q水经亚沸处理能显著提高ES细胞保持未分化状态的能力，优质胎牛血清能提高ES的AKP阳性度和克隆形成率。

Purification of Milli-Q water by sub-boiling distilled and employment of qualified FBS could increase the AKP staining positive level and total clone formation rate of mouse ES cells .

取第3代骨髓基质细胞，以含胎牛血清的DMEM培养液培养消化后，计数细胞量，描绘细胞增殖曲线。

After 3 passages were obtained , and then digested by DMEM medium containing fetal calf serum , cell amount was counted and the proliferation curve of MSCs was depicted .

方法：取材于出生2~3d小鼠颅骨，以含20%胎牛血清的DMEM培养液进行培养，采用胶原酶消化法分离成骨细胞，并进行细胞接种与传代。

Methods : The osteoblasts were harvested by collagenase from the calvaria of 2 ~ 3 day fetal mice , and cultured in DMEM containing 20 % fetal calf serum .

含10%胎牛血清的高糖DMEM作为普通完全培养基组，分别加入血小板血浆和血小板裂解液至终浓度为1%和3%作为条件培养基组培养BMSCs。

10 % FBS high glucose DMEM complete medium as an ordinary group , platelet lysate were added to a final concentration of 1 % , 3 % conditioned medium group training BMSCs .

方法：肠上皮细胞系Caco-2和T-84在37℃下用加10%胎牛血清DMEM培养基培养于12孔板中直至融合。

Methods : Intestinal epithelial cells Caco-2 and T-84 were cultured in DMEM medium with 10 % FBS at 37 ℃ until confluent in 12-well plate .

试剂和药品：DMEM培养基，牛胰岛素，庆大霉素，胎牛血清，DPBS均为GIBCO公司产品；

Reagents and drugs : DMEM medium , bovine insulin , gentamicin , fetal bovine serum ( FBS ) and DPBS were purchased from GIBCO Ltd.

浸提液的制作：按体积分数为0.1的胎牛血清DMEM液，与壳多糖-胶原凝胶表面积之比为10mL/cm2加入培养液，于4℃下静置24h。

Preparation of leaching liquor : According to 0.1 volume fraction DMEM liquor of fetal calf serum was moved into culture solution based on 10 mL / cm2 of chitosan-collagen gel at 4 ℃ for 24 hours .

用Ficoll-Hapaque分离法分离动员的外周血（MPB）单个核细胞，免疫磁珠法分离纯化CD34+细胞，并将其在含胎牛血清的液体培养体系中、各组细胞因子诱导下培养15天。

Mononuclear cells were isolated from mobilized peripheral blood ( MPB ) by density gradient centrifugation over Ficoll . CD34 + cells were purified by using an immunomagnetic bead separation system .

正常对照组和模型对照组在加入去除生长因子的血清DMEM/F12（含体积分数为0.2的胎牛血清）中培养7d进行诱导分化。

Samples in the normal control group and the model control group were cultured in serum DMEM / F12 ( containing 0.2 fetal calf serum ) without growth factor for 7 days to induce the differentiation .