穿梭质粒

大肠杆菌-酵母穿梭质粒的组装

Construction of E. coli - Yeast Shuttle Plasmid

信号标签诱变突变体文库穿梭质粒构建

Shuttle plasmid construction of Signature-Tagged Mutagenesis technology

为进一步筛选与登革病毒C蛋白可能存在相互作用的宿主蛋白，本研究分别以表达登革病毒C蛋白的酵母穿梭质粒及人脐带血内皮细胞cDNA文库作为诱饵载体和筛选文库。

Host proteins that interact with dengue virus C protein were screened from human umbilical cord blood endothelial cells cDNA library .

结论：构建的3种腺病毒穿梭质粒分别可以表达HCV不同段的结构基因，为包装表达HCV不同结构基因的腺病毒载体奠定了基础。

This should be useful to pack adenovirus expression vector which can express HCV structure genes .

利用穿梭质粒快速构建含抗HAVFab基因的重组杆状病毒

Rapid construction of recombinant baculovirus carrying anti-HAV Fab gene using a baculovirus shuttle vector

结核分枝杆菌抗原蛋白ESAT-6基因重组穿梭质粒的构建与鉴定

Construction and Identification of Recombinant Shuttle-plasmid with ESAT-6 from Mycobacterium tuberculosis

建立pESnx／FL穿梭质粒致突变测试系统的实验研究

Experimental Studies on the Development of pESnx / FL Shuttle Vector Plasmid Mutation Test System

用微量热法研究了尿嘧啶缺陷型酿酒酵母AY，和该菌株分别经穿梭质粒pYLZ-2、重组质粒pYLZ-2/f27、pYLZ-2/622转化的3种菌株的生长代谢热动力学。

By using microcalorimetry method , we have studied ura auxotrophic mutant of saccharomyces cerevisiae AY , and 3 strains transformed from saccharomyces cerevisiae AY by plasmids .

分枝杆菌融合表达ICL-GFP穿梭质粒的构建及鉴定

Construction and identification of shuttle plasmid expressing ICL-GFP fusion protein in Mycobacterium

但是，仅pESnx／FL克隆可获得每皿50个以上的转化子和低于10－4的自发突变率，符合穿梭质粒测试系统的要求。

The spontaneous mutation frequency was less than 10-4 in pESnx / FL. Therefore , pESnx / FL accord with the requirements of shuttle vector plasmid mutation test system .

将重组的穿梭质粒转化大肠杆菌并从大肠杆菌提取重组穿梭质粒转化卡介苗。

The BCG cells were transformed with the recombinant shuttle plasmid .

链霉菌和大肠杆菌穿梭质粒载体的构建

The Construction of Shuttle Plasmid Vector of Streptomyces and E. colt

运动发酵单胞菌和大肠杆菌间穿梭质粒的构建

Construction of Shuttle Plasmids between Zymomonas mobilis and Escherichia coli

携带葡糖基转移酶基因链球菌－大肠杆菌穿梭质粒的构建

The construction of Streptococcus-Escherichia coli shutter vector carrying glucosyltransferase gene

结果经酶切鉴定及基因测序证实重组三突变型穿梭质粒构建成功。

Result The recombinant was correctly constructed and restriction endonuclease analysis and PCR amplification .

携带融合基因穿梭质粒的构建及在人肝癌细胞中的表达

Construction of the shuttle plasmid vector for fusion protein gene and expression in Hepatocellular Carcinoma cells

3种丙型肝炎病毒结构基因腺病毒载体穿梭质粒的构建鉴定及表达

Construction , identification and expression of three kinds of shuttle plasmids of adenovirus expression vector of hepatitis C virus structure gene

用结核分枝杆菌HSP70启动子构建分枝杆菌穿梭表达质粒的研究

Construction of the Mycobacterium shuttle expression plasmid with Mycobacterium tuberculosis heat shock protein 70 promoter

在突变株的构建过程中，我们发现由于S.aureus细胞壁厚而致密而且穿梭重组质粒较大，使用目前的常规转化方法无法导入外源性DNA，限制了遗传操作的进行。

In the process of mutant construction , we found it difficult to transfer exogenous DNA into S. aureus cells during transformation because of the thick compact cell wall of S. aureus and the large shuttle vector we used .

卡介苗穿梭表达质粒pMSIL-2的构建及鉴定

Construction and identification of recombinant shuttle - plasmid with secretory interleukin-2

大肠杆菌-分枝杆菌穿梭表达质粒pY-α的构建

Construction of Escherichia coli-mycobacterium Shuttle Expression Plasmid pY - α

分枝杆菌穿梭表达质粒的构建及外源基因在分枝杆菌中的稳定表达

Construction of E.coli-Mycobacteria Shuttle Expression Vector Plasmid and Stable Expression of Foreign Gene in Mycobacteria

人颗粒溶素活性肽和小鼠白细胞介素-12基因真核穿梭共表达质粒的构建与真核表达

Eukaryotic expression and identification of recombinant shuttle plasmid for co-expression of human granulysin active peptide and murine IL-12