克隆实验

结果用不同刺激细胞诱导产生的效应细胞能有效杀伤刺激细胞来源的靶细胞，进一步克隆实验证实这种杀伤效应是由CD8+细胞毒性T淋巴细胞（CTL）所致。

Results The effective cells induced by different stimulator cells could effectively lyse the target cells from the stimulators . Further cell clone study proved that the cytotoxic activity of the effective cells was caused by CD8 + cytotoxic T lymphocytes ( CTL ) .

成克隆实验测定细胞存活分数。

Clonogenic assay was performed to determine the survival fraction .

细胞克隆实验证实细胞有自我更新能力。

Clonal and population analyses showed their extensive self-renew capacity .

克隆实验开始于20世纪50年代，对象为青蛙和蟾蜍。

The first cloning experiments in the 1950s involved frogs and toads .

其他常用试剂均参照《分子克隆实验指南》配制。

Other agents were prepared according to Molecular Cloning .

请设计一个硅晶基因克隆实验，从其他植物（如玉米，水稻）找出它的同种异体基因。

Please design a gene cloning in silico process for its homologous genes from other plants ( such as maize , rice ) .

在单细胞克隆实验中，将培养板分为两组：第1组采用纯新鲜培养液，第2组为新鲜与原代培养液各半的混合液。

In the single cell clone experiments , the culture plates were divided into two groups : pure fresh serum-free medium was added into each well of the first group ;

本文以体外培养的宫颈癌Hela细胞系为模型，以细胞克隆形成实验及细胞周期相分布测定为指标，观察了高温、顺铂及放射线对细胞的杀伤效应。

In this paper the killing effects of thermo-chemo-radiotherapy were observed in Hela cells .

应用细胞克隆形成实验观察氨磷汀对宫颈癌Hela细胞放射敏感性的影响。

Using cell colony-forming experiment to observe the radiation protective influence of amifostine to cervical cancer Hela cells .

细胞常规培养，平板克隆形成实验和MTT法检测各组细胞的增殖活性。

Each group cells were cultured under normoxia . The cell proliferation was detected by colony formation assay and MTT assay . 4 .

方法应用锥虫蓝排染法、生长曲线测定法、克隆形成实验检测药物对HL-60细胞生长的影响；

Methods Cell inhibitory effect were determined by trypan blue dye exclusion test , growth curve method and colony formation .

克隆形成实验表明沉默Bmi-1基因的表达明显抑制了A549细胞的单细胞增殖能力。

Colony forming test demonstrated the single-cell proliferative capability of A549-siRNA-Bmi-1 cell was significantly inhibited .

克隆形成实验、MTT法和裸鼠皮下瘤形成实验分别检测细胞克隆形成率及细胞在体外和体内的增殖情况。

The proliferation of HeLa cells in vitro and in vivo was determined by clone formation assay , MTT assay , and subcutaneous tumor formation in nude mice .

软琼脂克隆形成实验及划痕法检测BGC-823克隆形成能力和迁移能力的变化。

Test clone forming and migration ability of BGC-823 by Soft-agar colony forming assay and wound-healing assay , respectively .

软琼脂克隆形成实验检测长期培养的药物存活细胞的克隆形成能力，MTT实验检测细胞对阿霉素的耐受能力。

Moreover , the colony forming ability of long term cultured drug survival cells was estimated by soft agar assay , while the doxorubicin tolerance was estimated by MTT assay .

用克隆形成实验检测抑制Annexina2基因沉默对MDA-MB-231细胞克隆形成能力的影响。

Colony formation assay was used to observe the influence of Annexin a2 on colony formation rate of MDA-MB-231 cells . 4 .

克隆形成实验分别检测DDP、TSA+DDP处理C13的克隆形成率；

The clonogenic rate of C13 cells after DDP or TSA + DDP treatment was determined by clonogenic test .

方法：1.MTT法和软琼脂克隆形成实验，检测宫颈癌Hela细胞的细胞体外增殖能力和细胞集落形成能力。

To investigate the cell vitro proliferative capacity and colony formation efficiency of cervical cancer Hela cells by MTT assay and soft agar colony formation assay . 2 .

通过平板克隆形成实验检测不同浓度芹菜素对Huh-7细胞克隆形成能力的影响。

Detected the ability of cell clone by flat plate clone formation test . 3 .

利用内盐法（MTS）、软琼脂克隆形成实验、细胞划痕愈合实验等分别分析了Rack1的稳定敲低对A549细胞增殖能力、克隆形成能力、迁移能力的影响。

The cellular proliferation , colony formation ability , migration capacity were detected by MTS assay , soft agar colony formation assay , and wound healing assay , respectively . 4 .

采用MTT比色法、平板克隆形成实验、流式细胞术和MTS比色法分析SLP鄄2对TE12细胞的影响。

MTT assay , plate clone formation assay , flow cytometry , and MTS assay were performed to measure the effect of SLP-2 on TE12 cells .

收集含药血清，通过倒置相差显微镜观察、四噻唑氮蓝比色分析法、平板克隆形成实验、细胞黏附分析实验和细胞侵袭分析实验研究白术提取物含药血清对PG细胞增殖、转移能力的影响；

To investigate the effect of extract of Largehead Atractylodes Rhizome on the proliferation and metastasis , through inverted phase contrast microscope 、 MTT colorimetric analysis antigenic 、 cloning experiment 、 adhensiveness experiment and invasiveness experiment ;

平板克隆形成实验提示：转染了C2基因和空载体的两种SGC7901细胞的克隆形成率分别为43.8%和76.9%（P<0.05）。

The average clone formation rate of C2 gene transducted cells is 43.8 % , the rate which is lower than that of vector transducted cells ( 76.9 % ) .

对于体外，通过CCK-8和克隆形成实验检测了AKBA对癌细胞的生长抑制情况。

In vitro assay , we performed the experiments of CCK-R and clonogenic formation to determine the inhibitory effect of AKBA on cancer growth .

采用光镜、电镜观察形态学变化，双层软琼脂克隆形成实验、MTT细胞生长曲线实验及裸鼠皮下接种实验观察转染后细胞体外生长情况。

Morphological changes of cells were observed with optic and electron microscopes . In vitro growth of the 7721 IGF R AS cells was observed with soft agar test , MTT test and with nude mice inoculation test in vivo .

软琼脂克隆形成实验和裸鼠成瘤实验也表明miR-365抑制胃癌细胞的成瘤能力。

Soft agar cloning assay and xenograft results revealed that miR-365 could inhibit tumorigenesis of gastric cancer cells .

方法：以5-FU作用Tb细胞24h后，应用MTT比色法与平板克隆形成实验观察Tb细胞的增殖活性，同时以全细胞膜片钳技术检测Tb细胞膜电位与跨膜钾电流的改变。

METHODS : After treatment with 5-FU for 24 h , the proliferation of Tb cells was determined by MTT and clone formation assay , the membrane potential and potassium channel current of Tb cells were recorded by whole-cell patch clamp technique .

克隆形成实验表明纳米金对人肺腺癌细胞株A549具有放射增敏作用，初步探讨其机制可能为抑制细胞亚致死损伤的修复阻滞细胞于G2/M期，并诱导细胞凋亡。

Through clonal survival , there was radiotherapy enhancement for Glu-GNPs on lung adenocarcinoma cell line A549 , and the mechanism may are restrain of repair of sublethal damage , blocking cells at G2 / M and inducing apoptosis .

克隆形成实验分析不同浓度多西紫杉醇对Hela细胞生长抑制的影响，多靶单击拟合模型方程测定的各组细胞放射增敏比，评价增敏效果。

Inhibitory effect of different concentration of docetaxel on Hela cell line growth was analyzed using clony formation test , and radiosensitizing effect through sensitizing enhancement ratio ( SER ) of different group cell line was measured with multi-target single-hit model .

目的：探讨洋地黄毒甙体外抗肿瘤作用。方法：以台盼蓝排染法、中性红活染法和克隆形成实验测定洋地黄毒甙（DT）对多种人癌细胞株的细胞毒作用。

Objective : To investigate antitumor effect of digitoxin in vitro , Objective : The cytotoxic effects of digitoxin on various human tumor cell lines were determined by trypan blue dye exclusion test , neutral-red vital staining method and colony-forming assay .