克隆载体

首先，构建克隆载体：将改造的人胰岛素样生长因子（huIGF-I）基因直接克隆到T-载体，并进行DNA序列测定；

First , cloning vector wais constructed : The gene encoding human insulin-like growth factor-I was modified .

图1.3列出了引出一段外源DNA进入克隆载体的大概步骤。

An outline of the procedure for introducing a piece of foreign DNA into a cloning vector is shown in Figure 1.3 .

以pBS+为克隆载体，构建圈养大熊猫基因组DNA的小插入文库。

The small - insert genomic DNA library was constructed from giant panda genome .

大鼠钠尿肽B受体cDNA探针克隆载体的构建

Construction of cDNA probe clone vector for natriuretic peptide B receptor

结果：构建成TA克隆载体。

Results : The recycled TA clone vector was demonstrated efficiently .

细菌人工染色体（bacterialartificialchromosome，BAC）是第二代大片段DNA的克隆载体系统。

Bacterial artificial chromosome ( BAC ) vectors are the secondary system for large DNA fragment cloning .

目的：再生TA克隆载体。

? Objective : To recycle TA clone vector .

因此，我们构建了新的克隆载体即T载体，专门用于克隆未经任何修饰的PCR产物；

Therefore , a new cloning vector ( T-vector ) was constructed , and specifically used to clone unmodified PCR products .

结论：①成功地构建了含大鼠TIMP1反义基因片段的TA克隆载体；

Conclusion : ① Rat antisense TIMP-1 gene TA cloning vector was constructed successfully .

通过RAPD数据分析和克隆载体构建技术共获得了4条特异性探针的克隆载体，为后续试验奠定了基础。

We got 4 clone of specificity probe by RAPD data analysis and cloning technology . 4 .

结论成功地构建了HLA-DRα基因片段TA克隆载体。

Conclusion : The HLA-DR α gene TA cloning vector was contracted successfully .

TA克隆载体的再生

Recycling of TA clone vector

方法：根据CD和TK基因DNA的序列分别设计、合成引物，构建克隆载体pMD18-T-CD和pMD18-T-TK，并进行DNA序列分析；

Methods : The primers of CD and TK were designed and composed at the base of the CD and TK ′ s DNA sequence .

结果：①目的片段克隆载体与p21基因启动子cDNA序列高度同源。

RESULTS : ① The cloning vector of target fragment was highly homologous with p21 gene promoter in cDNA sequence .

我们用常见的克隆载体pBR322作探针，用核酸点杂交和Southern吸印方法，检测了来自173人的213例样品。

We detected 213 specimens from 173 people using probe of pBR-322 by Dot and Southern blot hybridization .

目的构建含大鼠金属蛋白酶组织抑制物1（tissueinhibitorofmetalloproteinase1，TIMP1）反义基因片段TA克隆载体，为进一步研究TIMP1在肾组织纤维化中的作用奠定基础。

Objective To study the role of TIMP 1 in renal fibrosis , we constructed rat antisense tissue inhibitor of metalloproteinase 1 ( TIMP 1 ) gene TA vector .

然后通过各种渠道收集含有这些转基因元件的克隆载体或表达载体，并设计了相应PCR引物扩增保守区段，将其制备为探针。

Then we tired to collect the clone vectors or expression vectors that contain these transgenic elements via all kinds ways . Thus the conservative fragments were amplified and purified as probes .

可转化人工染色体（TAC）克隆载体及其在果树基因工程研究中的应用前景

Transformation - competent Artificial Chromosome ( TAC ) Cloning Vector and its Application Prospects in Fruit Genetic Engineering

细菌人工染色体是1992年以来发展起来的一种新型克隆载体，用于构建人、动物、植物核基因组DNA大片段插入文库。

Bacterial artificial chromosome ( BAC ) as a kind of new vector has been developed since 1992 . They have been used to construct large fragment insert libraries of genome DNA in human , animals and plants .

采用RT-PCR法从活化的人T细胞中扩增了ICOS全长基因，装入T-vector克隆载体。

Full-length of ICOS was amplified through RT-PCR from activated human T cells and the cDNA fragment was cloned into T-vector plasmid .

目的建立风疹病毒包膜糖蛋白E1的克隆载体；研究E1基因变异情况，并对其序列进行系统发生树分析。

Objectives To construct the cloning vector of glycoprotein E1 gene of rubella virus , to study the mutation of glycoprotein E1 gene , and to analyze the sequence of E1 gene by the phylogenetic tree .

跨膜蛋白基因克隆载体pMBL的构建及验证

Construction and Verification of the Effectiveness of pMBL : A Cloning Vector of Exported Proteins Encoding Genes

目的：构建马动脉炎病毒读码框ORF5、ORF6、ORF7主要结构蛋白基因全长片段的克隆载体及汉逊酵母穿梭载体，并获得重组工程菌，为下一步酵母表达重组目的蛋白奠定基础。

Objective : To construction the cloning and yeast expression vectors containing ORF5 、 ORF6 、 ORF7 structural protein gene of equine arteritis virus and lay foundation for expressing these recombinant protein .

戊型肝炎病毒基因片段开读框架3（369bp）的克隆载体及真核表达载体的构建

Construction of clone and expression vector of HEV ( ORF3 ) gene

一种新的质粒克隆载体的稳定性和拷贝数的测定

Stability and copy number in host bacteria of a new plasmid cloning vector

大片段克隆载体研究进展

Progress in Development of Large DNA Fragment Cloning Vectors

构建Neuregulin1克隆载体的实验

Construction of a neuregulin 1 cloning vector

利用增强型绿色荧光蛋白基因作筛选标记克隆载体的构建及鉴定

Construction and identification of a screening vector using enhanced green fluorescent protein ( EGFP ) as an indicator

然后以重组克隆载体质粒为模板，扩增β-防御素5基因成熟肽片段。

Then , the β - defensin 5 gene mature peptide fragment was amplified according the recombinant clone vector plasmid .

结果酶切证实目的DNA定向克隆至载体上，测序分析结果与目的序列相同。

Results The target DNA was directly cloned to vector and the result was correct by sequence analysis .