克隆技术

DNA克隆技术是分子生物学研究中一项重要的技术手段。

DNA cloning technology is the basic terrace for molecular biology .

使复制完全相同dna成为可能的技术，就是我们所说的克隆技术。

The technology that makes it possible to reproduce the exact same DNA is what we would call " Cloning Technology " .

Windows下的批量遥控克隆技术

Technique for Clone Grouped Clients by Remote Control under Windows

通过分子克隆技术，MinD融合在该荧光蛋白的N端。

MinD is fused in the N-terminus of the fluorescent protein by molecular cloning techniques .

基于分子杂交和单分子PCR的同源基因克隆技术

Homology Cloning Based on Molecular Hybridization and Single Molecular PCR

利用Gateway克隆技术大规模克隆拟南芥转录因子

High Throughput Cloning of Arabidopsis Transcription Factors Using Gateway Cloning Technology

果树核DNA提取、目的基因分离与克隆技术研究进展

Advances in the techniques for extraction of nuclear DNA and isolation and cloning of target genes in fruit trees

基于PCR的cDNA基因克隆技术研究进展

Research progress on PCR-based techniques of cDNA gene cloning

涉及基因工程的dna研究还包括克隆技术，即对生物组织进行基因复制。

Many of the possibilities for D-N-A research involve genetic engineering , including the creation of clones genetic copies of organisms .

Ghost克隆技术在图书馆系统维护中的应用

Ghost cloning technology and its application in library system maintenance

本文报道了应用DNA重组和分子克隆技术研究雄激素与大鼠储精囊分泌蛋白基因的相互作用。

Androgen regulation of the secreting protein synthesis in Rat Seminal Vesicle was studied by means of DNA recombinant and Molecular cloning technique .

一组基于RT-PCR的未知产物基因克隆技术及其在植物方面的应用

A set of RT-PCR-based techniques for PRODUCT-UNKNOWN gene cloning and their applications in plants

目的通过基因克隆技术获得饰胶蛋白基因cDNA克隆并表达。

Objective To study Decorin biological effects , Decorin gene cDNA was cloned and expressed in E.

目的应用重组和克隆技术在大肠杆菌中表达葡萄球菌肠毒素B（SEB）突变体。

Purpose To clone and express staphylococcal enterotoxin B ( SEB ) mutants in E. coli .

为了打破这一瓶颈，panda等在2000年引入了感染性克隆技术。

To study it deeply , panda etc inducted the infection clone technique in 2000 .

采用一种简单高效的互补脱氧核糖核酸（cDNA）克隆技术制备人胰岛细胞瘤的cDNA文库。

A cDNA library of human insulinoma was constructed by using a highly efficient and simplified cDNA cloning method .

优化AFLP阳性带回收克隆技术

Optimizing recovery and cloning of positive bands in AFLP analysis

利用DNA合成技术、DNA克隆技术、PCR扩增技术以及DNA芯片技术，结合密码学的计算复杂度理论，提出了一种基于DNA技术的加密方法。

Using the technologies of DNA synthesis , DNA clone , PCR and DNA chip and the theory of computational complexity , an encryption scheme is formed .

为了避免上述问题，本文改造了突变株及回补株构建过程中使用的质粒，使其可以利用Gateway克隆技术进行克隆构建工作。

To avoid the above problems , the plasmids utilized for mutant and complemented strain construction were modified to be Gateway-compatible .

方法：主要为PCR和TOPO-TA克隆技术。

Methods : Mainly by means of PCR and TOPO-TA cloning .

方法利用分子克隆技术将14个不同亚型的中国旱獭IFNα家族基因亚克隆到真核表达载体，将中国旱獭IFNα5亚型基因亚克隆到原核表达载体。

Methods Eukaryotic and prokaryotic expression plasmids harboring Chinese marmot interferon alpha gene with different genotypes were generated using molecular cloning technology .

较详细地综述了DNA水平上森林植物分子生态学的研究方法：DNA分子标记、DNA测序、基因克隆技术、DNA芯片技术的的原理和方法；

This article describes the researching approaches for forestry plant molecular ecology on DNA level , namely , DNA molecular markers , DNA sequencing , and techniques in gene cloning and gene chip .

近年通过克隆技术鉴定了组胺H3和H4亚型受体。

A third histamine receptor subtype was characterized in recent years .

科学家们本来希望应用治疗克隆技术来制备神经元和其他的成熟细胞。该技术可将患者的DNA插入到捐献的卵子中，以得到胚胎干细胞。

Scientists had originally hoped to create neurons and other adult cells using " therapeutic cloning ," in which DNA from a patient is inserted into a donated egg to create embryonic stem cells .

染色体微切割与微克隆技术首先在动物和人类上得到应用，随着PCR技术的发展，该技术在植物遗传与进化研究等方面也得到了较为广泛应用。

Chromosome microdissection and microcloning technology was first used in animal and human . With the development of PCR , they was applied in plant genetic and evolutional researches widely .

结论应用噬菌体表面呈现技术和基因克隆技术构建的特异性抗HCC噬菌体ScFv具有抑制HCC细胞生长、促进HCC细胞凋亡的功能。

Conclusion The phage display ScFv that against to HCC could inhibit HCC cells ' growth and prompt HCC cells ' apoptosis . $

结论：用抑制性差减杂交及T/A克隆技术成功构建了高脂血症敏感兔差异表达基因差减cDNA文库。

Conclusion : A subtracted cDNA library of differentially expressed genes in hyperlipidemia sensitive rabbit liver was constructed successfully by SSH and T / A cloning techniques .

在此基础上利用电子克隆技术克隆出3个黄瓜雌性表达相关基因的cDNA全长，并分别对其组织特异性表达进行了研究。

On this basis , cloned full-length cDNA of the three genes expressed related cucumber female expression , and its tissue specific expression , respectively , were studied .

方法采用TA克隆技术并进行碱基序列测定，通过GeneBank进行同源分析。

Methods TA cloning technique was utilized and the product was sequenced and analysed for homology sequence with GeneBank .

基于PXE网络克隆技术的探讨与实现

Study and Realization of Network Clone Based on Pxe