杂交细胞

根据人类基因组X染色体上FMR1I、DS、FATE、BGN、F8A等5个基因的信息资源，用已构建的猪/仓鼠96个放射杂交细胞系分析猪染色体该5个基因间的连锁关系。

In this study , based on the information of five human genes ( FMR1 , IDS , FATE , BGN , F8A ) on the X chromosome , the linkage relationship of these five genes in pigs were analyzed by a panel of 96 radiation hybrid cell lines .

小鼠杂交细胞生物学特征的观察

The observation of biological characteristics of hybrid cell in mice

融合系统pH值对杂交细胞集落形成率的影响

Influence of pH in Fusion System on Plating Efficiency of Hybrid Cells

AL杂交细胞在放射生物学研究中的应用

The application of AL hybrid cells in radiobiology study

方法通过PEG介导制备SP2/0BALB/c小鼠脾细胞杂交细胞。

Methods The hybridoma cell strain was established from SP2 / 0 cells and the splenocytes of BALB / c mouse by PEG mediate technique .

方法应用辐射杂交细胞系（RH）定位的方法进行染色体定位，应用绿色荧光蛋白（GFP）基因作为报告基因，结合荧光显微摄影进行蛋白质定位。

Methods Chromosome locus was defined by radiation hybrid mapping method and protein localization by green fluorescence protein ( GFP ) as reporter molecule .

对杂交细胞各代进行同样的原位杂交检测，可见癌基因的表达均有不同程度的减弱，其中尤以C-myc和Ki-ras癌基因受到抑制的现象最为明显。

On the other hand , the expression of oncogenes in hybrid cells was found to be dramatically decreased , among them , the oncogenes of c-myc and Ki-ras been suppressed completely .

其中，C17杂交细胞株，从它冰冻保存的第20代细胞复苏后，又传30多代，并接种在小鼠腹腔内增殖，仍能分泌特异性抗体。

Among them , C17 line had been frozen at the 20th generation before passing through 30 generations and inoculating into abdominal cavity of BALB / c mice .

胰岛素对杂交细胞生长的影响

The Effect of Insulin on The Growth of Hybrids

肿瘤杂交细胞的抗癌免疫试验

Immunization of rats by tumour hybrid cells

中国仓鼠核仁形成区的活性在仓鼠-小鼠杂交细胞中受抑制

Suppression of Nucleolar Organizer Regions Activity of Chinese Hamster Cells in Mouse-hamster Somatic Hybrid Cells

人早幼粒白血病细胞与小鼠网织红细胞融合的胞质杂交细胞形态学观察

Morphological observation of cybrids derived from the fusion of human promyelocytic leukemia cells with mouse reticulocytes

细胞融合与形成杂交细胞是细胞工程的一项基本操作。

The fusion of cells to form a hybrid cell is a basic procedure in cell engineering .

杂交细胞能被应用于剖析真核细胞中控制基因表现的调节机理。

That cell hybridization can be used to dissect regulatory mechanisms controlling gene expression in eukaryotic cells .

结果提示：肿瘤杂交细胞在一定程度上能阻抑或延迟二乙基亚硝胺诱发大鼠肝癌的发生。

The results showed that immunization with tumour hybrid cells could inhibit or delay DEN hepatocarcinogenesis in rats to a certain extent .

长期培养的胞质杂交细胞沿不同途径向较成熟的方向分化。

The cybrids which cultivated for long period in vitro developed into more mature cells along both myeloid and erythroid differentiation pathway .

结果表明，电离辐射能够刺激抗体分泌细胞分泌抗体的能力，对研究机体辐射损伤时免疫活性细胞的功能变化和探讨增强杂交细胞分泌单克隆抗体的能力具有参考意义。

These results indicate that ionization radiation depresses the survival activity of hybridoma cells , but stimulates the secretion of monoclonal antibody of survival cells .

果与电镜和流式细胞光度计的实验结果一致，表明珠蛋白基因产物血红蛋白住杂交细胞中，能够表达并成为融合细胞的标志。

Results indicated that the resultant cybrid cells are characterized by the appearance of hemoglobin and its expression might be served as a marker for the cybrids .

恶性和正常细胞的杂交细胞体外抑制恶性亲本细胞浸润的观察

The observation of the inhibition of the invading capacity of malignant parental cells ( Eca 109 ) by the hybrid cells of malignant cells ( Eca 109 ) and normal cells ( CHO ) in vitro

低熔点胶包埋法制备分子杂交用细胞基因组DNA

Preparation of cellular genomic DNA for molecular hybridization by the method of LMP agarose gel plug

8－氮鸟嘌呤诱变3D5杂交瘤细胞的初步研究

The primary study of mutagenesis of hybridoma 3D 5 against human erythrocyte with 8 azaguanine

无牛血清IgG细胞培养基（-GFCS培养基）的制备及其在杂交瘤细胞体外培养中的应用

Preparation and application of-GFCS medium to culturing hybridoma cells in vitro

抗链霉素杂交瘤细胞株的建立与竞争ELISA试剂盒的研制

Filtration of hybridoma lines and development of rapid test competitive ELISA kit for streptomycin

分泌抗LDH-C4pIgA和IgG抗体的杂交瘤细胞在生殖道的归巢

Homing of hybridoma cells secreting pIgA and IgG against LDH-C4 to genital tracts

获得3株稳定分泌IgM型天然抗角蛋白单克隆抗体的杂交瘤细胞株。

Three hybridoma strains secreting natural IgM monoclonal antibody against keratin were obtained .

抗人IgM单克隆抗体的研究&脾内免疫建立抗人IgM单克隆抗体杂交瘤细胞株

Studies on Monoclonal Antibody against Human IgM & Establishment of McAb Hybridoma cell

用纯化的蛋白包被ELISA反应板，对融合成功的杂交瘤细胞进行筛选。

The ELISA plates were coated with purified protein and hybridoma culture supernatants were screened by indirect ELISA method .

用建立间接ELISA方法检测杂交瘤细胞上清，结果阳性率为20.6%。

The supernatant of hybridoma cells were detected by indirect ELISA , and the positive ratio was 20.6 % .

采用间接ELISA试验和HI试验双向检测杂交瘤细胞分泌的抗体，阳性率为78.4%；

Our screening of the hybridoma cells by indirect ELISA and HI , the positive rate is 78.4 % ;

植物抗体基因工程：I.分泌马铃薯Y病毒中和抗体杂交瘤细胞系的筛选

Antibody Genetic Engineering in Plant : I.Selection of a Hybridoma Cell Line Secreting Monoclonal Antibody Against Potato Virus Y