悬浮培养

在悬浮培养过程中pH值变化趋势先降低，后升高。

During suspension culture , the pH value firstly decreased and then increased .

大蒜细胞悬浮培养生产SOD的初步放大

Primary scale-up of SOD production by garlic cell suspension culture

悬浮培养细胞生长速率与培养液的pH值存在一定的相关性。

There are a certain interrelation between the callus growth rate and the suspension pH value .

用高效液相色谱检测结果显示，银杏圆铃品种的悬浮培养细胞中银杏内酯B最高含量可达细胞干样质量的0.0758%。

The result by HPLC detection showed ginkgolides in suspension cells reached 0.0785 % of dry mass of cells from the variety Yuanling .

pH值、激素对新疆紫草悬浮培养细胞生长及紫草宁衍生物合成的影响

Effects of pH value and hormones on cell growth and shikonin derivative formation in suspension cultures of Arnebia euchroma cells

悬浮培养ES细胞能得到囊状胚体。

Culturing single suspension ES-like cell formed cystic embryoid body .

结果①脂质体转染试剂对悬浮培养的k562细胞转染效率较低。

Result The efficiency of liposomal reagent was low for suspended k562 cells .

方法骨髓细胞半固体培养和悬浮培养，DNA凝胶电泳，DNA缺口末端标记（TdT），电镜观察。

Methods Semisolid culture , DNA agarose electrophoresis , in situ terminal deoxynucleotidyl transferase assays , view with electromicroscope .

由细胞悬浮培养获得的太子参皂苷的HPLC色谱峰值与常规种植及组培苗的相同，但纯度较好。

The HPLC chromatograms of saponin extracted from suspension cell were consistent with those from conventional cultivation or tissue culture seedlings .

结果悬浮培养细胞在MS培养基中细胞生长良好，细胞分散性和喜树碱积累优于在其他培养基中。

Results Suspension culture cells grew well in MS medium and cell homogeneity and camptothecin content were superior to those in other media .

HPLC检测结果显示，银杏悬浮培养细胞中银杏黄酮含量可达细胞干质量的2.82%。

The result from HPLC analysis shows that the content of flavone glycosides of dry weight is 2.82 % in suspension cell .

Hela细胞高密度悬浮培养的研究

High Density Suspended Culture of Hela Cells

DMSO对悬浮培养的东北红豆杉细胞凋亡和紫杉醇合成的影响

Effect of DMSO on Apoptosis and Taxol Biosynthesis in Suspension Cultured Taxus cuspidata Cells

Vero细胞微载体悬浮培养制备人用狂犬病疫苗的研究

Study on Preparation of Rabies Vaccine by Using Vero Cell and Microcarrier System

在开始的几天中，悬浮培养中的细胞呈对数生长，即细胞鲜重、干重和DNA含量（表明细胞分裂）快速增加。

Cells in suspension culture show logarithmic growth , with rapid increase in fresh weight , dry weight and DNA content ( indicating ceil division ) over the first few days .

二次回归旋转设计在石刁柏（Asparagusofficinalis）细胞悬浮培养条件研究中的应用

Application of experimental design of quadric rotational regression in study on cell suspension culture for Asparagus officinalis

比较不同MS培养基浓度对悬浮培养的影响发现，MS是最佳的培养液浓度，光照对悬浮细胞的生长有明显的促进作用。

Comparison of MS medium with different concentrations of suspension cultures , MS is the best medium concentration . Light is significantly promoted on the growth of suspension cells .

对红豆杉悬浮培养细胞中脂氧合酶（LOX）在诱导子诱导紫杉醇合成中的作用进行了探讨。

The role of lipoxygenase ( LOX ) in the elicitor induced taxol production was investigated .

拟南芥细胞悬浮培养物实验表明UV-B和CRY1信号传递途径在动力学上和药理学上不同。

Experiments with an Arabidopsis cell suspension culture show that the UV-B and CRY 1 signaling pathways differ kinetically and pharmacologically .

建立了稳定的甘草细胞悬浮培养体系，选择MS基本培养基，接种量为10%，培养基的初始pH值为5.8。

Therefore , a stable Glycyrrhiza inflata Batal cell suspension culture system were established in which the MS medium , 10 % inoculum concentration , pH 5.8 as the initial culture pH were selected .

FAK的磷酸化水平在悬浮培养的细胞中是下降的，这与文献报导一致。

Phosphorylation of FAK was decreased in suspended cells , which is in agreement with previous reports .

ES细胞定向分化为心肌细胞的经典方法是悬滴培养-悬浮培养-贴壁培养三步法。

There are many methods of mouse ES cells directional differentiation into cardiomyocytes . The classic method is " hanging drop culture-suspension culture - adherent culture " .

濒危植物四合木（TetraenaMongolicaMaxim.）悬浮培养下体细胞胚胎发生

The development of somatic embryo of rare endangered Tetraena mongolica maxim . under cell suspension culture

烟草细胞悬浮培养生产CoQ（10）的动力学研究

Kinetics study on CoQ_ ( 10 ) production in tobacco cell suspension cultures

B5液体培养基较适合桑品种大10细胞的悬浮培养；

The results showed that medium B5 was comparatively fit for suspension culture of cells from mulberry variety Da 10 ;

HPLC法测定经酸水解的菊花中三种活性黄酮苷元的含量（英文）三种柚叶悬浮培养愈伤组织黄酮类有效成分的比较研究

An efficient method for the simultaneous determination of three flavone aglycones in Flos Chrysanthemi by acid hydrolysis and HPLC A Comparative Study of the Effective Components of flavonoids from Suspension-cultured Callus Among Three Citrus grandis Cultivars

按此滴度感染悬浮培养的昆虫细胞，收获、纯化VLP并鉴定其特异性；

With this titer infected the suspensively cultured insect cells , harvested and purified VLP and identified its specificity .

刺激剂对细胞悬浮培养的影响主要表现为酶活化、蛋白质含量改变、氧迸发、细胞程序性死亡、pH值改变、次生代谢产物积累等防御反应。

Defense responses are manifested mainly on cell suspension cultures by elicitor influence , such as enzyme activation , changes of protein content and pH , oxidative burst , programmed cell death , accumulation of secondary metabolites , et al .

方法：对出生1～3d小鼠耳蜗以体外悬浮培养方法，并用扫描电镜对其外毛细胞的结构和形态进行观察。

Methods : The free floating method was used to cultivate the cochleas of 1 to 3 days post natal mice in vitro . The structure and morphology of outer hair cells were investigated with scanning electron microscope ( SEM ) .

二甲基亚砜（DMSO）渗透处理三七悬浮培养细胞，使该细胞有效释放胞内皂甙。

Dimethy sulfoxide ( DMSO ) was used to permeate suspension cells of Panax notoginseng for effective release of a useful intracellular secondary metabolite ginseng saponin .