贴壁培养

结果1.通过淋巴细胞分离液以及贴壁培养分离的方法，得到骨髓间质干细胞。

Human bone marrow mesenchymal stem cells were get by lymphocyte separation medium and adherent culture .

采用贴壁培养法培养大白猪体外成纤维靶细胞，并进行各项生物学特性的检测。

Cultured Big White Pig fibroblast Cell by adherent culture method , and detection its biological characteristics .

进行MSCs的体外培养和传代扩增。采用贴壁培养法使MSCs得到纯化。本实验通过对T。

MSCs were cultured , proliferated and purified by passage culture . We cultured T.

结论：用密度梯度离心和贴壁培养法可以分离并培养得到bMSCs。

Conclusion : bMSCs can be obtained from bone marrow by the density gradient centrifugation method .

通过密度梯度离心与红细胞裂解后联合贴壁培养法可获取较为纯化的MSCs。

MSCs were purified by the method of split red blood cell , combining gradient density centrifugation and adhesion separation .

方法：将抽取的兔子骨髓用密度梯度离心和贴壁培养法分离bMSCs，在体外进行细胞的原代和传代培养，观察细胞的生长情况。

Methods : bMSCs were extracted from the bone marrow in rabbits by density gradient centrifugation method and cultured in vitro regularly .

方法：利用机械与酶消化的方法获得均一性的兔髁状突软骨细胞，在二维贴壁培养条件下增殖至P2代；

Methods : The cultured mandibular condylar chondrocytes of neonatal rabbits was released by mechanical dissection and enzyme digestion .

α-MHC和ANP在贴壁培养的8天有表达，12天和16天时表达逐渐增多。

ANP and α - MHC expressed at 8th day , 12th and 16th day , and the expression was gradually increased .

我们现在报道的体外诱导ESC系R1分化形成神经细胞的方法是在单层贴壁培养系统中引进RA来实现的。

Here we report an in vitro adherent culture system to induce mouse ESC line R1 into neural cells accompanied with RA .

方法将12～24周人胚眼的视网膜色素上皮细胞（RPE）自脉络膜毛细血管床上撕离，置入培养皿中作贴壁培养。

Methods RPE patches were obtained from human fetal eyes aged from 12 ~ 24 weeks and cultured in regular culture dishes .

ES细胞定向分化为心肌细胞的经典方法是悬滴培养-悬浮培养-贴壁培养三步法。

There are many methods of mouse ES cells directional differentiation into cardiomyocytes . The classic method is " hanging drop culture-suspension culture - adherent culture " .

30%～70%Percoll梯度离心，10%FBS贴壁培养4～6h洗去非贴壁细胞。

Then gradient centrifugation were done with 30 % 70 % Percoll and cultured in 10 % FBS for 4 6 hours .

采用差速贴壁培养法分离脂肪源间充质干细胞，并与普通培养法得到的脂肪源间充质干细胞进行CD44阳性鉴定和用流式细胞仪检测细胞的CD44阳性率。

Were isolated by differential adhesion and primary culture . And contrasted their CD44 masculine . The F2 adipose tissue-derived mesenchymal stem cells .

1995年Gage建立了NPCs的贴壁培养方法，大大推动了对NPCs的相关研究。

In 1995 , Gage established adherence culture method for NPCs which has promoted the related researches on NPCs greatly .

材料与方法1.1滑膜细胞贴壁培养及在明胶支架中培养：滑膜取自TMJ髁突肥大无滑膜病变的手术患者。分离出平滑光亮的滑膜组织，Ⅱ型胶原酶消化后。

Materials and Methods : Complex culture of cell and scaffold : The synovium is separated from joint in surgical patient of condylar hyperplasia .

贴壁培养的神经前体细胞，群体倍增时间为（22.9±2.7）h，传代、冻存和复苏对细胞形态及生长无明显影响。

The population doubling time of INPC was ( 22.9 ± 2.7 ) h , subculture , freezing and recovering had no effect on cellular shape and proliferation of INPC . Conclusion Immortalized neural progenitor cell strain was established successfully .

依据贴壁培养法分离和扩增成骨细胞，噻唑蓝比色法（MTT）法检测原代细胞细胞增殖率绘制细胞生长曲线，取前4-6代细胞作为研究对象。

Adherent culture based on isolation and amplification of osteoblast , detected the cell proliferation rate by MTT assay and draw growth curve.4-6 generation of cells were taken into study . 2 .

方法：先用IV型胶原酶和胰蛋白酶消化出生3天的SD鼠臂层神经和坐骨神经组织块后再将组织块贴壁培养。

Methods The sciatic and brachial plexus nerve of three-day-old SD mice was treated with collagenase type IV and trypsin to remove the desmocyte on the Schwann cell , then explanted in the culture disk .

为了深入开展对NPCs相关研究，本课题在完善NPCs贴壁培养方法的基础上，对比研究了两种培养条件下的NPCs的生物学特性，为深入开展NPCs的基础研究和临床应用研究提供实验依据。

This subject has studied biological characteristics of NPCs cultured by two kinds of methods comparatively on the basis of perfecting the adherence culture method .

结果：精原细胞主要分布于45%～55%梯度间的Percoll中，经贴壁培养后纯度达75.2%。

Results : Spermatogonia were mainly distributed in Percoll gradient between 45 % ~ 55 % , purity of spermatogonia was 75.2 % after being purified .

方法：将贴壁培养的肝癌细胞株BEL7402分成对照组、缺氧24h组、缺氧48h组、缺氧加照射组和单纯照射组。

METHODS : The BEL7402 liver tumor cells were divided into the control , hypoxia 24 hours , hypoxia 48 hours , hypoxia plus radiation and radiation alone groups .

方法：1：选取SD大鼠，体重约80g，无菌条件下取股骨骨髓，采用密度梯度离心与贴壁培养相结合的方法分离、纯化得到BMSCs，流式细胞仪检测BMSCs表面标志物。

Methods : 1 , BMSCs were isolated from bone marrow of rats with density gradient centrifugation and adherent culture . Expression of the BMSCs surface marker was detected by flow cytometer .

这些结果与单层贴壁培养系统中诱导R1细胞神经发生时的状况一致，暗示了iPSCs与ESCs具有相似的神经发生能力。

Such results were consistent with that of R1 cells when it cultures in adherent monoculture system . It also means that iPSCs share the similar neural differentiation ability of ESCs .

材料和方法1.用密度为1.073g／ml的淋巴细胞分离液以及贴壁培养分离骨髓间质干细胞，培养、传代并冻存储备细胞。

Get the human bone marrow mesenchymal stem cells by lymphocyte separation medium ( 1.073g / ml ) and adherent culture . Store cells for further study by passage and freeze cells .

将小鼠5d龄ES-D3细胞源的类胚体（EBs）单细胞，按5×104/mL接种6孔细胞培养板，在DMEM基础培养基中使EBs单细胞贴壁培养。于第14！

The cells of 5-day embryoid bodies ( EBs ) derived from murine ES-D3 lineage were inoculated into each well of 6-well culture plate at 5 × 104 / mL in DMEM .

RT-PCR分析结果显示：转染组GATA-4在贴壁培养的4天有表达，8天、12天表达逐渐增多，16天时表达有所下降。

RT-PCR analysis showed that , in transfected group , GATA-4 expressed at 4th day after adherent culture . At 8th day and 12th day , the expression of GATA-4 was gradually increased but decreased at 16th day .

【方法】采用密度梯度离心和贴壁培养法分离大鼠MSCs，反复传代及纯化后，取第3代MSCs，采用流式细胞仪检测细胞表面抗原CD34和CD44；

【 Methods 】 MSCs were isolated from adult SD rats , and then were cultured and cloned by density gradient method and adhesive cultivation . The cell surface antigens of CD34 and CD44 in the third generation of MSCs were detected with flow cytometer .

结论密度梯度离心结合贴壁培养法可获得高纯度MMSCs，成人MMSCs在传代培养的增殖活性随年龄增加而降低。

Conclusion High purity MMSCs can be obtained by gradient centrifuging and adherent culture . The proliferation activity in passage cell culture of adult MMSCs is reducing while age increasing .

结果免疫磁珠分离获得NGFR阳性细胞的纯度为（90.6±5.1）%，NGFR阳性细胞较贴壁培养获得BMSCs具备更强增殖能力和向软骨分化潜能。

Results The purity of NGFR + cells obtained by immunomagnetic selection was ( 90.6 ± 5.1 ) % . NGFR ~ + cells proliferated faster and had stronger potential of neurogenesis differentiation than BMSCs separated by routine culture .

通过密度梯度离心和贴壁培养相结合的方法在体外提取、扩增培养小鼠骨髓间充质干细胞（MSCs），并经流式细胞学检测各代CD29、CD34、CD45的表达情况。

The mouse bone marrow-derived mesenchymal stem cells ( MSCs ) were separated and cultured with the combination of density gradient centrifugation and adherent culture , and the expressions of CD29 、 CD34 、 CD45 in MSCs were detected with flow cytometry . 5 .