杂交测序

求解杂交测序问题主要有精确算法（分支定界算法，动态规划方法等）和启发式搜索方法（禁忌搜索算法，遗传算法，蚁群算法，模拟退火算法等）两类。

Methods which solve the SBH problem are exact algorithms ( branch and bound algorithm , dynamic programming method , etc. ) and heuristic methods ( tabu search algorithm , genetic algorithm , ant colony algorithm , simulated annealing algorithm , etc. ) .

DNA杂交测序法

DNA Sequencing by hybridization

基于并行ACO算法的DNA杂交测序

DNA Sequencing By Hybridization Based on Parallel Ant Colony Optimization Algorithm

本实验采用PCR扩增DNA全长，并通过Southern杂交对测序结果进行验证，试验结果与基因组测序结果一致，进一步证明了南方根结线虫基因中编码Ras蛋白基因序列只有一个。

PCR amplification and Southern blot were conducted so that to confirm the sequence results , which were coincide with each other . The results of above proved that there is only one gone encoding Ras protein in M. incognita .

间期荧光原位杂交结合测序分析在慢性髓系白血病慢性期伊马替尼治疗监测中的应用

Application of interphase fluorescence in situ hybridization and sequencing analysis in imatinib treatment of patients with chronic myeloid leukemia in chronic phase

方法采用聚合酶链反应反向点杂交、DNA直接测序方法检测了45例瘢痕疙瘩患者与60名正常对照的p53基因第72位密码子多态性位点的基因型。

Methods The p53 genotypes were determined by polymerase chain reaction-reverse dot blot ( PCR-RDB ) and DNA direct sequencing in 45 patients with keloid and 60 unrelated healthy controls .

将反相斑点杂交方法结果与测序结果进行比较，评价反相斑点杂交方法检测rpoB基因突变的准确度，并且与比例法药敏试验结果进行对比，评价二者的一致性。

The results of the two method were compared to evaluate the accuracy rate of reverse-line blot hybridization assay .

酵母双杂交阳性克隆经测序后显示共存在9段不同序列（存在重复克隆）。比对氨基酸序列得到5个可能的相互作用蛋白。

Nine different sequences by sequencing the positive clones ( There are repeated clones ) and five alternative proteins were obtained by the amino acids sequence blast .