金龟子绿僵菌

用gfp基因标记法研究金龟子绿僵菌在玉米根际的定殖动态

Colonization of Metarhizium anisopliae in Corn Rhizosphere as Studied with gfp Marker Gene

通过正交设计试验优化了绿僵菌RAPD-PCR反应体系，并对不同地理来源金龟子绿僵菌进行DNA多态性RAPD分析。

Optimized RAPD-PCR reaction system by orthogonal design , and DNA polymorphism for RAPD analysis of Metarhizium anisopliae isolate from different areas .

测定了不同温度和pH及碳氮源、微量元素对金龟子绿僵菌菌丝生长及产孢的影响。

A strain of Metarhizium anisopliae was incubated in solid culture under different conditions of temperature , pH , carbon and nitrogen sources .

金龟子绿僵菌（Metarhiziumanisopliae）是在害虫生物防治中起着重要作用的一类重要的昆虫病原真菌。

Metarhizium anisopliae is playing an important role in biological control of pests of an important class of insect pathogenic fungi .

目的：对金龟子绿僵菌的代谢产物进行初步的研究，验证其产物中苦马豆素（swainsonine，SW）的存在，为SW的来源提供新的研究思路；

Subject : Provide a new thoughtfulness for preliminary studying the source of swainsonine ( SW ) and validate SW in the metabolism products of Metarhizium anisopliae .

金龟子绿僵菌（Metarhiziumanisopliae）能侵染多种鳞翅目昆虫，广泛用于生物防治，是最常用的生物农药之一。

For its large host-range which includes many kinds of lepidoptera and its large scale use for bio-control , Metarhizium anisopliae have been one of bio-pesticides for using widely .

产烟曲霉酸等甾型抗生素的真菌，例如烟曲霉和金龟子绿僵菌等，具有优良的甾体羟化和C1,2位脱氢能力。

Aspergillus fumigatus and Metarhizium anisopliae , fungi which produce steroid antibiotics like helvolic acid , have a great capability for steroid hydroxylation and C1,2 dehydrogenation .

经过优化，得到金龟子绿僵菌产孢的最佳生物参数是：接种量为2.5g/100g、种龄为5-6天；

The technology of two - stage fermentation was also studied . The results showed that optimal biological conditions of solid - state fermentation of Metarhizium anisopliae were as following : inoculation ratio 2.5g/100g and seed age 6 days .

金龟子绿僵菌孢子制备、贮存及检测标准化研究

Standardization of Preparation , Storage and Inspection of Metarhizium Anisopliae Conidia

金龟子绿僵菌对甾体底物11-α羟化反应的工艺

11 - α hydroxylation Process of Steroids with Metarhizium anisopliae

通过对菌株形态学鉴定，获得金龟子绿僵菌29株。

The strains were identified of Metarhizium anisopliae by morphology . 2 .

金龟子绿僵菌菌丝体及发酵液中苦马豆素的提取与分离

Isolation and Extraction of Swainsonine from Mycelium and Fermentation Broth of Metarhizium Anisopliae

金龟子绿僵菌固体培养条件的筛选

Screening of solid culture condition for Metarhizium anisopliae

一种新的金龟子绿僵菌酪氨酸蛋白磷酸酶的纯化、特性及基因克隆

Purification , Characterization and Gene Cloning of a Novel Protein Tyrosine Phosphatase from Metarhizium Anisopliae

用查氏蛋白胨培养基对金龟子绿僵菌进行发酵培养，收集发酵液；

Fermentation cultured Metarhizium anisopliae on Czapek ′ s medium and collected the fermented broth ;

金龟子绿僵菌固态培养生物变量优化研究

Study on Optimization of biological variables in the solid - state fermentation of Metarhizium anisopliae

结果表明，本试验对金龟子绿僵菌的筛选、发酵及应用具有理论和实践意义。

The result showed that the test for Metarhizium anisopliae in screening , fermentating and applying was of great significance .

金龟子绿僵菌是一类重要的生防真菌，在生产上已得到广泛应用，全世界约有200多种昆虫能被绿僵菌的不同菌株感染致死。

Anisopliae is an important biocontrol fungus and there are 200 kinds of insects that can be infected by different strains of Metarhizium .

采用薄层色谱、气相色谱等方法对提取物进行初步鉴定分析；②金龟子绿僵菌发酵液提取物的体外抑菌活性试验。

Production was identified by TLC and GC method . ② Experimental study on antimicrobial effects of extractives from Metarhizium anisopliae in vitro .

应用金龟子绿僵菌来防治红棕象甲，为红棕象甲的综合防治开辟一条新的途径。

The application of Metarhizium anisopliae to combat the Red Palm Weevil opens up a new way for the integrated control of the Red Palm Weevil .

目前，世界上已经有十多个金龟子绿僵菌菌株登记注册，并实现了商品化，但这些产品常常不稳定。

At present , many strains of M. anisopliae have been registered in the world and have realized commerciality . However , these products usually show instability .

本研究以东亚飞蝗和金龟子绿僵菌（M.anisopliae）菌株CQMa102为研究材料，首次用高通量、高分辨率的双向电泳技术在感病昆虫血淋巴中筛选出了13个差异表达糖蛋白点。

For the first time , 13 differentially-expressed glycoproteins were screened from locust haemolymph glycoproteins mycosed with Metarhizium anisopliae by high throughput and high resolution 2-DE ( two-dimensional electrophoresis ) technology .

金龟子绿僵菌侵染寄主昆虫时，分生孢子通过分泌多种蛋白酶类降解昆虫体壁，并同时在体表萌发形成附着胞，附着胞的形成与分化在侵染过程中发挥着重要作用。

Metarhizium anisopliae infect a host insect , the spores secrete various proteinases degrade insect cuticle , and also in the formation of surface appressorium germination , appressorium formation and differentiation in the infection process plays an important role .

金龟子绿僵菌中烟曲霉酸合成基因簇的克隆和基因功能分析金龟子绿僵菌是甾体药物羟化反应的常用菌，具有较高的烟曲霉酸生产水平。

Cloning of helvolic acid gene cluster of M. anisopliae and analysis of its gene functions . M. anisopliae , commonly used strain in the hydroxylation reaction of steroid drugs , has a high level of helvolic acid production .

金龟子绿僵菌首先穿透寄主体壁侵入体内，在血淋巴定殖，再分泌大量毒素并掠夺寄主营养致死寄主，最终是孢子在体内成功定殖的情况决定着侵染的结果。

Metarhizium anisopliae send through the main wall of the first invasion in vivo , in the hemolymph colonization , plunder the host by secreting toxins and nutrients to the host death , ultimately determines the outcome of infection is successful colonization of spores in vivo situation .