体外扩增

小鼠树突状细胞的体外扩增培养及形态观察

In vitro amplification and morphology of dendritic cells from mice

核酸体外扩增技术的新发展

Development of in vitro amplification of nucleic acids

DNA体外扩增结果良好。

The result of PCR for the DNA was good .

DNA体外扩增技术&聚合酶链式反应（PCR）

DNA Amplified Technique Out body Polymerase chain Reaction ( PCR )

结论双链引物PCR是一种特异性更好的DNA体外扩增技术。

Conclusion The double strand primers reported here may increase the specificity of PCR amplification .

目的比较不同来源的树突状细胞（DC）在体外扩增、分化成熟的特点。

Objective To compare the growing behavior of dendritic cell ( DC ) from different sources .

经α-半乳糖酰基鞘氨醇体外扩增Vα24自然杀伤T细胞的生物学特性

Ex vivo Expansion of V α 24 Natural Killer T Cells with α - galactosylceramide

方法体外扩增培养、成骨诱导犬BMSCs。

Methods Isolated BMSCs were expanded in vitro and osteogenic induced .

造血细胞生长因子对脐血CD（34）~+细胞短期体外扩增的作用

Effect of Hematopoietic Growth Factors on Short Expansion of Umbilical Cord Blood CD_ ( 34 ) ~ + Cells in vitro

目的探讨核酸体外扩增（POLYMERASECHAINREACTION，PCR）技术在骨结核病诊断与鉴别诊断中的应用价值。

Aim : To evaluate the clinical value for polymerase chain reaction ( PCR ) technique in diagnosis and differentiating diagnosis of bone tuberculosis .

目的观察造血抑制因子TNFα对人脐血基质细胞体外扩增的效果。

Objective To study the effects of TNF α on human umbilical cord blood stromal cell ( HUCBSC ) colony .

结论采用添加IL-2培养体系是体外扩增Treg细胞的优选方法，其增殖效能强于IL-10；

Conclusion IL-2 is superior to IL-10 for Treg proliferation in vitro .

小剂量X射线照后TIL细胞体外扩增及抗癌作用的变化

Reaction of low does ionizing radiation on amplification in vitro and antitumor effect of TIL cells

乙肝病毒蛋白抗原表位特异性CTL体外扩增条件优化

Optimizing the conditions for in vitro expansion of HBV protein epitope-specific CTLs

方法：利用PCR技术，以HBV病毒DNA为模板，体外扩增HBVPreS／S基因。

Method : HBV preS / S gene fragments were amplified from HBV DNA in vitro , using PCR techniques .

MSC增加细胞因子的脐血细胞体外扩增的作用；

MSC enhance the effect of cytokines on cord blood cell expansion ex vivo .

聚合酶链反应作为一种强有效的DNA体外扩增技术，已被广泛地应用于一些遗传性疾病的临床基因诊断。

Polymerase Chain Reaction ( PCR ) as an efficient DNA amplification technique in vitro hasbeen widely al (?) lied to clinical gene diagnosis of some hereditary diseases .

研究结果如下：1、成功分离了大鼠BMSCs，并实现体外扩增培养。

BMSCs were isolated from rat marrow and culture successfully in vitro . 2 .

本实验建立了用聚合酶链式反应（POLYMERASECHAINREACTION，PCR）技术体外扩增哺乳动物SRY特异序列鉴别哺乳动物胚胎性别的方法。

A Polymerase Chain Reaction ( PCR ) - based method for sex determination of mammals embryos by amplification of mammals SRY-specific sequence was established .

人神经生长因子cDNA的克隆&人神经生长因子cDNA的体外扩增及纯化

Molecular Cloning of cDNA of Human Nerve Growth Factor : The Amplification in Vitro and Purification of cDNA of Human Nerve Growth Factor

PHA对CIK细胞体外扩增影响的研究

Experimental study on effect of PHA on proliferation of CIK cells in vitro

CIK细胞的体外扩增及其抗肿瘤特性的研究

Study of the expansion in vitro and its anti-tumor character on CIK cell

抗人CD3单抗重、轻链可变区基因的体外扩增、克隆和序列分析

Amplification , Cloning and Sequence Analysis of the Genes of V_H and V_K of an Anti-human CD3 Monoclonal Antibody

牛SRY序列的体外扩增

Cattle SRY - Specific Sequence Amplification

GM-CSF对脐血CD34~+巨核祖细胞体外扩增及分化的影响

Effect of GM-CSF on Expansion and Differentiation of CD34 ~ + Megakaryocyte Progenitor Cells from Cord Blood in vitro

结果成人骨髓MSC在体外扩增15代可获得（2～3）×1012个细胞。

Results Bone marrow MSC proliferated as undifferentiated cells in culture for 15 passages and ( 2-3 )× 10 12 cells were obtained .

根据水稻的保守区设计引物，利用PCR体外扩增技术，从大米草中克隆Na+/H+逆转运蛋白基因片段。

A segment of Na ~ + / H ~ + antiporter gene from English cordgrass was cloned by PCR using a pair of primers designed according to the conserved domains of rice .

目的体外扩增培养兔骨髓间充质干细胞（MSCs）。定向诱导MSCs为类Schwann细胞。

Purpose : To cultivate rabbit bone marrow mesenchymal stem cells ( MSCs ) in vitro and induce it to differentiate into cells with Schwann cell characteristics .

ADSCs体外扩增和自我更新能力很强，传代培养易于获得大量有分化能力的细胞。

ADSCs have strong ability to self-renewal and mass culture accessible to a large number of cell differentiation in vitro .

转FL、GM-CSF基因的人骨髓基质细胞促进脐血CD34~+细胞体外扩增

Effects of hFL and / or hGM-CSF gene transfected human bone marrow stromal cell lines on expansion of CD34 ~ + cells from cord blood