革兰氏染色

本文用淋球菌培养比较研究对革兰氏染色法在女性淋病诊断中的应用作重新评价。

This study re-evaluated the application of Gram 's stain in diagnosing gonorrhea in women by comparing with gonococci culture .

方法采用阴道分泌物涂片，革兰氏染色法，对483例经常冲洗阴道防治阴道炎的人群（观察组）和557例非经常冲洗阴道的人群（对照组），进行阴道乳酸杆菌半定量检测。

Methods Adopt vaginal fluid smear , Gram 's stain examination the half value of vaginal lactobacilli in 483 cases ( observation group ) who often washing vagina and in 557 cases ( control group ) who does not often washing vagina .

PCR检测法与革兰氏染色镜检法同时检测慢性淋病结果的比较

Comparison of Results of PCR and Gram Staining Microscopic Examination for Chronic Gonorrhea

革兰氏染色阴性；PAS阴性。关于非强平稳NA列的重对数律及其应用

On the laws of the Iterated Logarithm for nonstationary negative associated sequences and Its Applications

我们从病死猪的病料中成功分离到三株猪链球菌分离株，经革兰氏染色、生化试验、血清凝集试验和PCR鉴定，最终证实为猪链球菌Ⅱ型。

Three bacterial isolates from clinical samples were diagnosed as Streptococcus suis type ⅱ based on Gram staining , biochemical character , agglutination and PCR test .

Ⅱ32菌株在MRS培养基上可以生长，最适生长温度为28℃，革兰氏染色呈阳性，无芽孢。

The stain ⅱ 32 was gram-positive and non-sporing . It grew in MRS media and the optimum temperature was 28 ℃ .

菌株A5、X5的革兰氏染色结果分别为革兰氏阴性和革兰氏阳性。

Strain A5 and X5 were identified Gram-negative and Gram-positive .

试验结果显示，在对大肠杆菌进行革兰氏染色时，细菌在伊红美蓝培养基上培养的时间不宜超过24h；

The result of experiments shows that during Coliform Gram 's dye the time of culturing bacterium in EMB medium is within 24h ;

革兰氏染色金葡菌L型阳性率为70%。

The rate is 70 % with gram staining .

改良革兰氏染色检测男性尿道细胞涂片诊断NGU的价值

Use of the modified Gram 's staining to examine male urethra cell smears for diagnosis of non-gonococcal urethritis

从10个规模化猪场以直肠棉拭子采集PWD病料，经细菌分离纯化、革兰氏染色、生化试验和小鼠致病性试验等，对病原进行鉴定。

The isolations were identified by gram stain , biochemical reactions and pathogenicity tests in mice .

它与切片革兰氏染色L型检出阳性率（67.2%）无显著性差异，P＞0.05。

The results obtaines by culture and Gram 's staining accounted for 67.2 % presented no significantly statistical difference as compared with those mentioned above ( P > 0.05 ) .

幽门螺杆菌（HelicobacterPylori，H.pylori，Hp）是一种革兰氏染色阴性螺旋形的微需氧菌，其感染是诱发人胃粘膜病变的主要因素之一。

Helicobacter pylori ( H. pylori ) is a spiral , gram-negative and microaerophilic bacterium , and is one of the major factors inducing pathological changes in human gastric mucosa .

幽门螺杆菌（HelicobacterPylori，Hp）是一种定植于人类胃粘膜的革兰氏染色阴性、螺杆状、微需氧菌；在全世界范围内广泛感染，对人类健康构成严重危害。

Helicobacter pylori ( Hp ) is a gram negative , spiral , microaerobic bacterium that colonizes human gastric mucosa and infects a large amount of population worldwide , leading to specific gastric diseases .

染色特性为革兰氏染色阴性、未观察到芽抱、无鞭毛，细胞以二分分裂方式繁殖。革兰阳性菌中耐甲氧西林菌株中MRSE占第一位。

The cells strain is Gram - negative . MRSE is the first in gram positive bacteria .

对其进行了形态观察、革兰氏染色及各项生理生化指标鉴定，并和Biolog细菌鉴定系统鉴定的结果进行了比对。

The traditional morphology , physiological and biochemical properties as well as identified system of Biolog were applied to the bacteria classification .

从大棚土壤中分离到一株异养型硝化细菌，命名为菌株HN，分离菌株为革兰氏染色阳性，球状或杆状。

A heterotrophic nitrifier , named strain HN , was isolated from the greenhouse soil . The cells of isolates were Gram positive , rod or coccus .

KOH-Test方法快速判定菌株的革兰氏染色反应

Rapidly Detect Strains of Gram-Stain Reaction with the KOH-Test

结果在BV诊断中，唾液酸酶法阳性率为29.3%，革兰氏染色法为24.1%，两者之间有显著差异性（p<0.05）。

Results The sensitivity of sialidase determination was 29.3 % and Gram stained smears examination for clue cells was 24.1 % . Significant difference was found between them ( p < 0.05 ) .

最后给受试物之后24h无菌采取粪便，培养后观察菌落形态、革兰氏染色等鉴定计数每克湿便菌落数。

The fecal bacteria-free was collected before treatment and at 24 hours after the last treatment . The colony form was observed after culture , and the number of colony every gram fecal was evaluated by Gram staining .

菌株ZP在牛肉膏蛋白胨固体培养基上菌落为深绿色，产生水溶性绿色素弥漫整个平板，革兰氏染色阴性，呈杆状，无鞭毛。

The colony of ZP is bottle green on the solid culture medium of beef extract , because the bacterial strain ZP is able to produce water-solubility pigment .

小肠结肠炎耶尔森菌（yersiniaenterocolitica）是广泛存在于自然界的一种引起一系列肠道疾病的革兰氏染色阴性菌，是能在冷藏温度下生长的少数肠道致病菌之一。

Yersinia enterocolitica is a Gram-negative enteric pathogen responsible for a number of gastrointestinal disorders and is widely distributed in nature . The bacteria has the ability to multiply in foods at low temperatures .

本研究从海水中分离筛选到一株琼胶酶高产菌，编号是2.2-g，革兰氏染色为阳性，呈细杆状。

A straight Gram-positive bacteria containing highyield agarase was selected from sea water and named 2.2-g .

通过对这28株菌株的菌落形态观察、革兰氏染色和芽孢染色镜检，筛选出12株芽孢杆菌（Bacillus）菌株进行了生理化学鉴定，其中有11株分属于芽孢杆菌的3个种。

Though the observation of colonial morphology , Gram staining and spore staining , 12 strains of Bacillus were chosen to be identified physiologically and biochemically according to the diagnostic characteristics of Bacillus . Among those 11 strains were identified and classified as 3 species of Bacillus .

通过比较KOH-Test法和革兰氏染色法，指出KOH-Test法是快速判定菌株的革兰氏染色反应的方法。

A comparative study on the KOH-test with the Gram staining , we found that the KOH-test is a rapid method for distinction of Gram-negative from Gram-positive bacteria .

通过革兰氏染色、凝乳时间观察和产酸量分析，筛选出生长活力高，产酸能力强的2株酸乳生产菌株：SLlb和SLst。

Two strains of lactic acid bacteria ( SLlb , SLst ) were screened through Gram stain , curd time observation and acid production analysis .

方法：临床收集石蜡包埋的上胃肠道疾病患者胃镜活检或手术标本，采用革兰氏染色法和Warthin-Starry银染色法检测石蜡包埋胃组织中Hp的感染情况；

Methods : Collected the paraffin embedding pathological samples of upper gastrointestinal diseases from living examination of gastroscope or operation samples . Adopted Grams stain and Warthin-Starry silver stain to detect Helicobacter pylori infection states in stomach tissue . Collected the blood serum samples of upper gastrointestinal diseases .

在土壤中分离到一株细菌STRB，该菌可以在SFM、LB、EMB、PDA、MM、MacC等多种培养基上生长，革兰氏染色为阴性，电镜观察无鞭毛。

A bacterial strain named STR B was isolated from soil , which could be cultivated on some media , such as SFM , LB , EMB , PDA , MM and MacC agar . It was gram negative and could be viewed without flagellas under electron microscope .

通过菌落形态观察、显微镜观察及革兰氏染色等一系列生理生化实验，初步确定菌株E-9为大肠杆菌。

Through the observation of the character of individual form and the colony form , as well as the oxidase and a series of physiological and biochemical experiments , E-9 was identified as E. coli .

革兰氏染色结果的影响因素及原因分析

Related factors and reason analysis on result of Gram 's dye