随机引物法

PCR引物法及随机引物法标记cDNA探针杂交效率的研究

The Study on Hybridizing Efficiencies of cDNA Probes Labeled with PCR Priming and Random Priming

利用国外引进的重组质粒获得纯化基因片段，分别以随机引物法和PCR法制备地高辛素标记的HBVdna探针和HDVcDNA探针。

The fragments of HBV DNA and HDV cDNA , obtained from two recombinant plasmids , were labelled with digoxigenin by random priming and polymerase chain reaction methods , respectively .

随机引物法标记放射同位素DNA探针及其应用

Random primer extension radiolabeled DNA probe and its application

地高辛随机引物法标记探针的Southern杂交技术优化

Technological Improvement of Southern Blot Using Digoxigenin-labeled Probes with Random-primed Method

按随机引物法以地高辛标记HBVdna探针，与按缺口翻译法以~（32）P标记的探针进行了比较。

The whole HBV DNA sequences were labelled by random primer incorporation of Digoxigenin - labelled dUTP , and compared with ~ ( 32 ) P-labelled probe by nick translation .

利用DNA探针法和随机引物法从大黄粉虫的cDNA文库中筛选TmGRP的全部DNA序列。

Using DNA probe method and random primer method , the full DNA sequence of Tm-GRP is screened from the cDNA library of Tenebrio molitor .

结果：在斑点杂交中，正义引物PCR法所标记的单链探针的杂交效率最高，高于随机引物法一个数量级。

The results showed that : In dot blot , the hybridizing efficiency of probe labeled with the method of SPCR was the highest in the three methods , and 10 times higher than that of probe labeled with random priming .

方法：以随机引物法标记人perforincDNA探针，对慢性丙肝患者肝活检标本进行原位杂交，观察肝局部浸润炎性细胞perforinmRNA的表达及分布，分析他们与肝损伤之间的关系。

METHODS : The expression and distribution of perforin mRNA in the local infiltrated cells were detected by in situ hybridization using a dig labeled perforin cDNA probe in liver biopsy tissues .

对麦类作物的基因组DNA，宜采用随机寡核苷酸引物标记法进行生物素标记，适当延长标记时间可以提高标记效率。

Whereas the random oligonucleotide labelling is more suitable for genomic DNA probe and the labelling efficiency could be increased by prolonging the labelling time appropriately .

结果与结论：Taq酶标记法和大肠杆菌Klenow片段随机引物延伸标记法同样有较好的标记效果，且随机引物或特定引物作为延伸引物均可以合成足够有效的探针。

Results and conclusion : Taq DNA Polymerase labeling method is as efficient as Klenow fragment random primer DNA labeling system .

以大肠杆菌Klenow片断随机引物延伸标记法作为对照。

However , the essential content of law has its own form which is the inner structural character . coli Klenow fragment random primer DNA labeling system served as control .

白纹伊蚊乙酰胆碱酯酶基因片段简并引物PCR、克隆及鉴定随机引物聚合酶链反应法鉴定2例无菌性脑炎的未知病毒

Degenerate Primers Polymerase Chain Reaction Clone and Identification of the Acetylcholinesterase Gene Fragment from the Mosquito , Aedes albopictus ; Detection of Unknown Virus from 2 Case of Aseptic Meningitis Epidemic Using Random Primer PCR Assay

随机引物聚合酶链反应法鉴定2例无菌性脑炎的未知病毒

Detection of Unknown Virus from 2 Case of Aseptic Meningitis Epidemic Using Random Primer PCR Assay