基因组文库

动物遗传资源保存方法多种多样，如活体保种、干细胞库、突变体库、生殖细胞库、cDNA文库、基因组文库等。

There are many ways to achieve the purpose , such as saving living bodies , establishing stem cell bank , mutant bank , generative cell bank , cDNA library , genome library and so on .

大鼠APKD基因分子克隆研究（Ⅱ）大鼠基因组文库中APKD基因同源顺序的克隆&筛选与鉴定

Research on Rat APKD Gene Molecular Cloning (ⅱ) Construction of Rat Genome Library Screening and Identifying

构建丝状真菌大片段基因组文库载体DNA的制备

Preparation of Vector DNA for Filamentous Fungal Large-insert Genomic Library Construction

药用野生稻基因组文库构建与大片段DNA转化

Construction of a Genomic Library of Oryza Officinalis and Transformation of Large DNA Fragments

载体DNA的制备是构建大片段基因组文库的关键步骤之一。

Preparation of vector DNA is a crucial step to large-insert genomic library construction .

3利用新型载体构建基因组文库以进行DNA大片段的敲除。

Establishment large chromosome fragment deletion on the basis of genomic library constructed with novel cosmid vector .

对这些组织的基因组文库检测，未取得有关片段整合到猪DNA的确凿结论。

Examination of genomic libraries from these tissues yielded no conclusive information regarding integration of the fragment into porcineDNA .

弓形虫基因组文库的建立、特异克隆的筛选和弓形虫病的DNA诊断

Construction of a genomic DNA library of Toxoplasma gondii ( zs2 strain ), screening of specific clone and DNA diagnosis of toxoplasmosis

bacterialartificialchromosome（BAC）基因组文库是进行基因组研究的基础工作之一。

The bacterial artificial chromosome ( BAG ) library is the basic tool for the genome research .

HL-60细胞系基因组文库的构建

Construction of genomic library of HL - 60 cell line

利用一碳化合物（C1）细菌的分离及基因组文库的构建

Isolation of the C1-utilization Ability Bacteria and Construction of Their Genomic Library

运用PCR法在中国对虾的小片段基因组文库中快速筛选含有微卫星序列的重组阳性克隆。

Recombinant positive clones containing microsatellite sequences of Chinese shrimp , Peneaus chinensis were obtained through rapid screening small-size fractionated genomic libraries with PCR technique .

用巢式PCR（Nested-PCR）方法，以中华按蚊基因组文库为模板，克隆中华按蚊全长基因组DNA序列。

Complete genomic DNA sequence of Anopheles sinensis defensins gene was cloned from Anopheles sinensis Genomic Library by Nested-PCR . 3 .

采用的基因克隆策略是用Southern杂交对其邻近基因进行定位后构建基因组文库，再用斑点杂交从基因文库中筛选目的转化子。

The gene cloning strategy was to construct genomic library after location of its neighbouring gene by Southern blot and to screen the aim transformant by dot blotting .

一种新型的载体系统&细菌人工染色体（bacterialartificialchromosome，BAC）已经发展成为构建复杂基因组文库的重要方法。

A new vector system-BAC ( bacterial artificial chromosome ) has been developed into an important method for the construction of complex genomic library .

分别从RAPD，锚定PCR和磁珠捕获3种富集方法构建的基因组文库中获得6,23,19个微卫星位点。

After sequencing positive colonies , 6,23,19 microsatellite loci are obtained from libraries enriched by RAPD , anchored PCR and magnetic capture , respectively .

而且，由于在哺乳动物基因组文库构建中，克隆的大片段DNA在大肠杆菌中十分稳定，所以基于F质粒的克隆技术已经被广泛接受。

Furthermore , F-plasmid cloning technology has gained widespread acceptance for the construction of mammalian genomic libraries due to their stable maintenance of large foreign DNA inserts in E. coli .

用磁珠富集法从AFLP片段中分离微卫星DNA标记，可以避免基因组文库的构建和筛选这一繁重的工作，既节省时间又节约费用。

Microsatellite enrichment from AFLP fragments by magnetic beads can obviate the tedious work of library construction which save not only time but also expense .

获得高浓度、大片段、无偏好的土壤总DNA是土壤微生物群落结构研究和宏基因组文库构建的基础。

Isolation of DNA sufficient in purity , large in fragment and unbiased representation of the microbial diversity from natural environments is the basis for molecular ecology study on soil microbial community structure .

方法：PCR方法扩增部分β酪蛋白基因作为探针，用原位杂交和PCR方法从小鼠基因组文库中分离克隆β酪蛋白基因。

Methods : Using a partial fragment of β _casein gene obtained by PCR as a probe , a mouse genomic library was screened by in situ hybridization and PCR .

牦牛瘤胃微生物基因组文库构建与16Srdna生物多样性研究

The Genomic Library Construction and Molecular Microbial Diversity of Yak ( Bos Grunniens ) Rumen Contents Analyzed by 16S rDNA

利用双子叶植物花发育基因的保守序列筛选水稻的基因组文库或cDNA文库已发现70多个同源异型基因。

Now over 70 homeotic genes were isolated from rice by using the conserved sequences of the homeotic genes of dicots as probe screening the rice genomic library or cDNA .

以可转化人工染色体（TAC）载体为基础的百脉根基因组文库的构建及筛选

The Construction and Screening of Genomic Library Based on Trans - formable Artificial Chromosome ( TAC ) Vector in Lotus japonicus

然后在分子分类水平上，运用PCR扩增技术、质粒重组技术和克隆技术构建窖池原核微生物基因组文库，利用末端终止法测定16Srdna基因全序列。

On the base of molecular taxonomy , the 16S rDNA gene library of prokaryotic microorganisms in the cellar is constructed by polymerase chain reaction ( PCR ), recombinant plasmid construction and cloning .

采用LA-PCR方法，从上述盐藻步行基因组文库中扩增NR基因5′上游序列，测序并进行分析。

The 5 ′ - flanking region of the NR gene from genomic walking libraries of D. salina was amplified by LA-PCR .

它包括构建野生稻可转化基因组文库，利用RecA重组蛋白介导生物素标记的探针对文库进行磁珠富集，构建候选抗性基因富集文库。

The research includes the construction of wild rice transformation-competent genomic library and the RecA protein mediated biotinylated probes enrichment library .

方法利用同源重组技术，通过转座子上的GFP基因，从酵母基因组文库中，检测发生突变的菌株，从而筛选出酵母突变株。

Materials and Methods Homologous recombination technique was used to detect mutant yeast strains in yeast genomic library , with the green fluore - scent protein gene as the reporter gene in the transposon .

人胃癌mgc803细胞系基因组文库的构建。

The construction of genomic library from human gastric carcinoma cell line mgc80-3 .

水稻基因组文库的构建及YR-DNA的筛选

The Construction of Rice Genomic Library and Selecting of YR-DNA

并通过构建基因组文库的方法获得了其中8个基因（abf62S9除外）的全长序列。

By genomic library construction , eight full-length genes ( except for abf62S9 ) were obtained .