基因互补
- 网络gene complementation
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已发展的四种载体构建策略包括:基因取代,基因插入,融合抗原和基因互补。
The strategies that have been investigated for foreign gene expression in various virus based vectors include gene replacement , gene insertion , epitope presentation and gene complementation .
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将它们导入Dnd表型缺失突变株ZX1中进行基因互补实验,结果证明变铅青链霉菌and基因簇上的3个ORFs均为Dnd所必需。
All of three ORFs were demonstrated to be necessary for the Dnd phenotype .
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简要来说,这种阻断机制可以通过将需要的和目标基因互补的ATGC序列插入到CRISPR位点中来对其进行编程。
This blocking mechanism can , in principle , be " programmed " by incorporating into the CRISPR locus any desired A , T , G , C sequence that would match a target .
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通过基因互补试验,确定基因缺失突变株的代谢产物不是由其它因素影响的。
Genetic complementations to the mutant strains were subsequently carried out to confirm the metabolites were not influenced by other factors .
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方法:利用RT-PCR扩增9例APL患者和10例正常人外周血单个核细胞中29个TCRVα亚家族基因的互补决定区3(CDR3),了解TCRVα亚家族T细胞的分布;
Methods : The complementary determining region 3 ( CDR3 ) of TCR V α 29 subfamily genes in peripheral blood mononuclear cells from 9 APL patients were amplified using RT-PCR .
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实验结果表明,Hoechst33258是一种特异性较强的指示剂,能够有效地区分CK-19基因的互补与非互补序列。
The results show that , Hoechst 33258 is a strong specific indicator that can distinguish the complementary and non-complementary sequence of gene CK-19 .
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从大肠杆菌免疫的BALB/C鼠的脾脏中提取iRNA,经Oligod(T)纤维素柱用亲合层析法分离PolyA~+RNA,以此PolyA~+RNA为模板合成了免疫核糖核酸基因的互补DNA(cDNA)。
In order to constract iRNA gene library cDNA was synthesized . The iRNA was extracted from BALB / C mice immunized with E. coli , and through a oligo d ( T ) chromotography the poly A ~ + RNA was separated as the template for the cDNA .
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体外细胞学研究发现,一种与Hira基因具有明显互补序列的微小RNA(aae-miR-12)在感染和未感染Wolbachia的埃及伊蚊细胞中表达水平存在差异。
In vitro cytology study suggest that the expression of a micro RNA ( aae-miR-12 ) which has obviously complementary sequences were difference between Wolbachia infected and uninfected Aedes aegypti cells .
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在lef-3基因上游的互补序列有一编码127个氨基酸序列的不完全的阅读框。
Upstream the lef-3 there is a sequence encoded 127 amino acids , but it is a part open reading frame .
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从特殊配合力效应中看到,抗氟性与茧层量这两个性状的基因可能有互补作用,说明选育抗氟性强兼茧层重的品种或杂交组合是可能的。
The effect of the specific combining ability suggested that there was a complementary relation between the genes of the fluorine resistance and the cocoon shell weight .
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基因密码子与互补密码子使用的相关分析
Correlation Analyses of Codons and Corresponding Complementary Codons Usage
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紫云英根瘤菌菌株107exo基因簇的遗传互补分析
Genetic Analysis of a Cluster of exo Genes Required for EPS Synthesis in Rhizobium astragalus
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CI-7则由1对显性基因和1对隐性基因的互补作用控制。
The resistance of CI-7 was controlled by complementary action of one dominant gene and one recessive gene .
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研究结果为进一步确定候选基因,以及后续的SGP(t)基因功能互补验证、表达分析奠定了基础。
This study laid a foundation for further determining candidate gene , functional characterization and expression analysis of gene .
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此基因片段既不在Rep基因也不在CaP基因内,而是位于Rep基因互补链上的一段基因序列,其功能还需进一步研究。
This gene fragment is not neither in cap gene nor in rep gene , but located in the complementary strand of rep gene , and it will be studied further more .
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基因芯片是一种点有若干寡核苷酸探针的基片,每个点都含有序列特异的探针和目标基因互补结合。
Gene chip was a basic chip which had a number of oligonucleotide probes , each point containing complementary linked with the sequence-specific probe and the target gene .