缺口平移

原位缺口平移标记断裂DNA技术及其在检测流行性出血热尸检和实验动物组织中细胞凋亡和早期死亡的应用

In situ nick translation labeling of DNA strand breaks : its application in differential detection of apoptosis and dying cells in the tissues from patients and experimen-tally infected animals with epidemic hemorrhagic fever

报道了以原位缺口平移（ISNT）技术研究鼻咽癌细胞株CNE、CNE－2Z对γ射线的放射效应，以及用存活曲线验证ISNT的可靠性。

We evaluated the radiosensitivity of two nasopharyngeal cell lines which had been treated with γ - rays by in situ nick translation ( ISNT ) .

实验采用吖啶橙荧光染色法显示睾丸细胞DNA和RNA，黄递酶（NADPH-d）组织化学法显示睾丸细胞NOS，采用原位缺口平移技术检测睾丸生精细胞凋亡的数目。

The methods of staining the testis cellular DNA and RNA with acridine orange , staining NOS with NADPH d histochemistry method , and observing the apoptosis number with in situ nick translation ( ISNT ) technique are used .

目的：了解缺锌对海马发育影响的机制，通过原位缺口平移（ISNT）标记，观察缺锌对睾丸生精细胞凋亡的影响。

Objective : To study the changes of the testis apoptosis on zinc deficient rats will promote the understanding of the affecting molecular mechanism of zinc deficiency on the develpment and function of testis .

采用原位PANT（DNA聚合酶I介导的生物素标记的dATP缺口平移）及原位TUNEL（末端脱氧核苷酸转移酶介导的dUTP末端标记），分别检测DNA损伤的单链断裂及双链断裂。

DNA polymerase I mediated biotin dATP nick translation ( PANT ) and terminal deoxynucleotidyl transferase mediated dUTP nick end labeling ( TUNEL ) were adopted for in situ identification of single and double strand DNA fragmentation , respectively .

应用原位缺口平移法检测细胞凋亡。

Apoptotic cells were specifically stained using in situ nick translation .

原位缺口平移测定鼻咽癌细胞的放射敏感性

Evaluation of radiosensitivity of nasopharyngeal cancer cells by in situ nick translation

原位缺口平移法在检测睾丸组织凋亡细胞中的应用

The Application of in Situ Nick Translation Labeling Technique on Apoptotic Cell in Rat

采用原位缺口平移技术观察睾丸曲细精管中细胞凋亡数目的变化。

The apoptosis of spermatogenic cells was studied with in situ nick translation ( ISNT ) technique .