转染试剂

该试验设立两个对照组：空白对照（未加siRNA及转染试剂）及阴性对照组（只加转染试剂，无siRNA）。

The study consisted of two control groups ; blank control group treated with neither siRNA nor transfection reagent and negative control group treated only with transfection reagent .

D组：予空白质粒转染试剂瘤内注射，每日一次。

Group D : blank plasmid transfection reagent intratumoral injection once a day .

结果①脂质体转染试剂对悬浮培养的k562细胞转染效率较低。

Result The efficiency of liposomal reagent was low for suspended k562 cells .

结果单纯损伤组和Lipofectin转染试剂组7d时可见内膜明显增生，且有较多平滑肌细胞表达PCNA，CFos30min表达达高峰。

Results There was significant intimal hyperplasia and obvious PCNA expression of smooth muscle cells at 7 day in Lipofectin group and only injury group , the expression of c-fos reached peak at 30 minutes .

对重组质粒进行双酶切、PCR和测序验证后，采用融细胞膜类基因转染试剂Trans-EZ将重组质粒转染至APOE敲除鼠星形胶质细胞，并利用划痕致伤建立细胞损伤模型。

Recombinant plasmid was identified by enzyme digestion , PCR and sequence analysis . Recombinant plasmid was transfected into APOE knocked out astrocytes with Trans-EZ and an in vitro trauma model was produced by scratch injury .

结果表明：相同用量的两种转染试剂对293细胞、PK细胞和MDCK细胞的生长的影响远远小于对CEF细胞的影响；

The results showed that the growth of the CEF cells were greater effected than that of 293 cells 、 PK cells and MDCK cells when the same dosages of the two kinds of transfection reagents were used .

病毒感染BHK21细胞后，通过阳离子转染试剂将重组质粒导入感染细胞，在此基础上评价融合蛋白D2C-SN抗登革病毒感染的治疗效果。

The recombinant plasmids were then transfected into BHK-21 cells pre-infected with wild dengue 2 virus , and the effect on dengue virus multiplication was evaluated by examining the production of infectious virions .

新一代阳离子聚合物转染试剂（梭华-Sofast）转染效果研究

Study on the Transfection Efficiency of the New Cationic Polymer Transfection Reagent : Sofast

细胞转染试剂盒

Reagents for cell transfection

毒性实验结果显示这种复合物的毒性比商业化的脂质体转染试剂小。

Toxicity test showed that the toxicity of these complexes was lower than that of the commercial liposome reagent .

该段siRNA转染的毒性作用主要来自于转染试剂，与序列本身无明确关系。

The toxic effect of siRNA transfection principally comes from the transfection reagent .