稳定表达

HBV全基因C区突变株在永生化B淋巴母细胞系中的稳定表达

Stable expression of HBV C gene mutants in immortalized human B-cell lines

VEGF（165）在哺乳动物细胞中的稳定表达

The stable Expression of VEGF_ ( 165 ) in Mammalian Cells

转染小鼠可溶性B淋巴细胞刺激因子稳定表达细胞株的筛选与鉴定

Screening and Identification of Stable Transfectants of Mouse Soluble B Lymphocyte Stimulator

稳定表达野生型DNA聚合酶β的细胞系的建立

Establishment of a Cell line Stably Expressing Wild Type DNA Polymerase β

目的建立稳定表达点突变型DNA聚合酶β的细胞系。

Objective To establish a cell line expressing containing point mutant DNA polymerase β stably .

副黏病毒Tianjin株NP蛋白的稳定表达及其检测

Stable Expression of NP Protein of Paramyxovirus Tianjin Strain

在部分PCR检测阳性植株中检测到了GUS的稳定表达。

Stable GUS expressions were observed in some of the PCR-positive plants .

结论获得了整合HBsAg基因，并能够稳定表达HBsAg的人参细胞表达系统。

Conclusion An expression system of HBsAg in ginseng cells was constructed .

coli中获得稳定表达产物。

The expression of E. coli stably .

稳定表达外源野生型p53基因人胆囊癌细胞系的建立和鉴定

The establishment of stable transfection of human gallbladder cancer cell line with exogenous wild-type p53 gene

目的获得稳定表达丙型肝炎病毒（HCV）结构蛋白的哺乳动物细胞系，以便对HCV结构蛋白的性质及其基因免疫效果作进一步的研究。

Objective Expression of structural protein of hepatitis C virus in mammalian cells stably .

人白细胞介素2受体α链cDNA导入哺乳细胞及其克隆与稳定表达的研究

Studies on the Introduction and Stable Expression of Human Interleukin-2 Receptor a Chain cDNA in Mammalian Cells

结果CD和tk基因在QBC939/CD+tk细胞中获得稳定表达。

Results Double suicide genes were stably expressed in QBC939 / CD + tk cells .

基于N蛋白稳定表达细胞株的SARS-CoV间接免疫荧光检测方法的建立

Immunofluorescence assay for detection of antibodes against SARS-CoV using BHK-21 cells stably expressing recombinant viral nucleoprotein

基因转染法建立CyclinG2稳定表达的胃癌细胞株

Establishment of cyclin G2 stably-expressed gastric carcinoma cell by gene transfection

结果：获得了稳定表达NAP的重组酵母菌株。

Results : The yeast strains expressing NAP were identified .

筛选出稳定表达潮霉素B抗性的转化菌株，继代培养9代后仍有潮霉素抗性。

Transformants which could express hygromycin B resistance genes effectively and steadily were obtained . The transformants maintained resistance to hygromycin B even after subculturing for 9 times .

氨苄青霉素板筛选及PCR鉴定，挑出阳性克隆进行扩增、转染cos-7细胞并经潮霉素及有限稀释法筛选稳定表达克隆。

Stable expression stains were screened by Hygromycin B and limited dilution method .

人重组阿片受体样受体在CHO细胞稳定表达系统的建立

Stable expression of human opioid receptor-like 1 in Chinese hamster ovary cells

结论乙型肝炎病毒X蛋白具有生长因子样作用，可刺激肝癌细胞生长，在肝癌细胞中稳定表达乙型肝炎病毒X蛋白可增加肝癌细胞恶性表型。

CONCLUSION The X protein has growth factor like activities and can stimulate the growth of hepatocellular carcinoma cells and enhance its malignant phenotype .

RT-PCR、Western印迹证实该基因在2个单系的骨骼肌和肝组织中有稳定表达；

PC 1 gene was found to be stably expressed in skeletal muscle and liver in 2 lines by RT PCR and Western blotting .

对获得的转基因苗进行PCR检测共获得6株转基因植株，RT-PCR检测结果表明有3株外源基因得到稳定表达。

We got six transgenic plants by PCR . The results of RT-PCR showed that three transgenic plants can express steadily .

ICOS/Fc在CHO细胞中的稳定表达

Stable expression of ICOS / Fc in CHO cells

稳定表达鼠IFN-λ的CHO细胞系建立

Construction of CHO Cell Line Stably Expressing Mouse Lamada-Interferon

非神经源性细胞中tau蛋白的稳定表达及磷酸化

Overexpression and hyperphosphorylation of tau protein in stably transfected 293T cells

人G-CSFcDNA的克隆和鉴定及其在哺乳类细胞中的稳定表达

Molecular Cloning and Characterization of cDNA for Human G-CSF and Its Expression in Mammalian Cells

苏云金芽孢杆菌cryIA（c）Bt基因的合成及其在大肠杆菌中稳定表达

PCR-aided Synthesis and Stable Expression in E.coli of the cryIA ( c ) Bt Gene

结论：缺氧可以通过诱导野生型p53的突变使HIF-1α稳定表达。

CONCLUSIONS : The hypoxia could induce the mutation of p53 which increases the expression of HIF-1 α .

结果：稳定表达诱骗RNA的K562细胞增殖受抑，增殖抑制率为（62.73±12.92）%；

RESULTS : The proliferation rate of stably transfected K562 cells was inhibited by ( 62.73 ± 12.92 ) % .

稳定表达HIV-1病毒样颗粒的293细胞系的筛选及其稳定性

Screening and Stability of 293 Cell Line for Stable Expression of Virus-like Particles of HIV-1