慢病毒

阳性克隆经过PCR鉴定以及DNA测序鉴定后，进行慢病毒小量包装。

After PCR and sequencing identification , the positive clones were packaged into lentivirus .

线粒体细胞色素C氧化酶RNAi慢病毒载体的构建

The construction of lentivirus-mediated RNAi vector containing cytochrome C oxidase

慢病毒介导的RNA干扰抑制白介素-1Ⅰ型受体对关节软骨有保护作用。

Lentivirus mediated RNA interference on IL-1R I provide protect on articular cartilage in osteoarthritic rats .

westernblot方法外源筛靶、内源筛靶，选择最有效的靶点，并包装成慢病毒。

Western Blot to exogenous target sieve , endogenous target sieve to select the most effective target and to package into lentivirus . 4 .

慢病毒介导的生长抑素siRNA对小鼠生长轴激素的影响

The Effects of Lenti-siRNA Against Somatostatin on Hormones of Growth Axis in Mouse

HIV-1来源的慢病毒载体转染大鼠脊髓神经干细胞

Lentivirus vector deriving from human immunodeficiency virus-1 transfer to rat spinal cord neural stem cells

大鼠Nogo受体基因RNA干扰慢病毒载体的构建与鉴定

Construction and identification of a recombinant lentivirus harboring RNAi targeting rat Nogo receptor gene

慢病毒载体介导RNAi的研究进展

Advance in RNAi Mediated with Lentivirus-based Vectors

慢病毒介导bcr/abl基因RNAi对白血病K562细胞生物学特性的影响

Effects of lentivirus-mediated long term bcr / abl RNAi on biologic characteristics of human leukemia cell line K562

慢病毒载体介导人IL-12基因修饰树突状细胞体外抗肺癌作用的研究

Study of in Vitro Antitumor Effect of Lentivirus-mediated Interleukin-12 Gene Modified Dendritic Cells in Lung Cancer

目的：为分析慢病毒介导的转基因小鼠中外源基因整合位点的信息，应用反向PCR克隆整合位点序列。

Objective : To investigate the information of transgene integrations in mice mediated with lentiviral vectors , the inverse PCR was used to clone the sequences of integration sites .

编码t-PA基因慢病毒载体的构建及其制备体系的建立

T-PA Lentivirus Vectors Construction and Lentivirus Production

LMP1与绿色荧光蛋白融合基因慢病毒的制备

Production of lenti virus of LMP1 and GFP recombinant gene

LMP1基因重组慢病毒载体的构建及体外表达

Construction and identification of a recombinant lentivirus vector of latent membrane protein 1

慢病毒介导EPO基因治疗肾性贫血大鼠的实验研究

Experimental research on EPO gene therapy of anemia caused by chronic renal failure by means of lentivirus mediation in rat

探索出了一套高效快速的慢病毒介导的小鼠ESCs基因修饰的方法。

Thus , a fast and efficient method for mouse ESCs genetic modification was developed .

马传染性贫血病毒（EquineinfectiousanemiaVirus，EIAV）属反转录病毒科慢病毒属，是马传染性贫血（EIA，简称马传贫）的病原体。

Equine infectious anemia virus ( EIAV ) is a lentivirus within the family Retroviridae and the causative agent of equine infectious anemia ( ElA ) .

子宫颈癌细胞株表达kiss-1基因重组慢病毒的构建

Cervical Cancer Cell Line in the Kiss-1 Gene Expression in Recombinant Virus Construction Slow

目的构建cathepsinK（CTSK）基因慢病毒表达质粒，为进一步研究cathepsinK在人软骨细胞体外老化过程中的作用奠定基础。

Objective To construct a cathepsin K ( CTSK ) lentivirus plasmid for exploring the function of cathepsin k in human chondrocytes aging in vitro .

仓鼠纹状体接种慢病毒scrapie后脑的代谢改变

Changes of Cerebral Metabolism after Scrapie Inoculation in the Striatum of Hamster Using Autoradiographic Method

结论成功构建了带有大鼠BMP-7基因的慢病毒载体，并实现其在HSC-T6的稳定高表达。

Conclusion Lentiviral vector carrying BMP-7 gene has been successfully constructed and maintains high expression in HSC-T6 cells .

我们以HIV-1来源的慢病毒载体为代表综述了慢病毒载体的结构特点，发展，及其应用研究进展。

In this paper , taking the Lv derived from HIV-1 for example , we review the characteristics of frame , the developments and the advances in application of Lv .

与其它慢病毒一样，在免疫压力作用下，EIAV基因组易于发生变异。

The same as the other lentivirus , under the immune pressure , EIAV gene tend to variation .

慢病毒介导的RNA干扰能有效的抑制DCs表面DC-SIGN分子表达，DC-SIGN表达降低对DCs成熟度及DCs诱导的下游免疫功能无影响。

The expression of DC-SIGN on the surface of DCs could be inhibited effectively by lentivirus-mediated RNA interference , but the maturity and the function of DCs were not influenced . 3 .

牛免疫缺陷病毒（Bovineimmunodeficiencyvirus，BIV）在分类上属于反转录病毒科的慢病毒属，目前尚未见BIV感染人的报道。

Bovine immunodeficiency virus ( BIV ) is a kind of Lentivirus belonging to Retrovirus , and up to now , there is no report about its infection to human .

结论：1、转染携带有HLA-G慢病毒作为治疗性的手段用于大鼠肝移植急性排斥反应模型，能有效保护大鼠移植肝的功能和延长存活期。

As a therapeutic means for acute rejection model , HLA-G carried Lentivirus could effectively protect the function of rat liver graft and prolong survival days . 2 .

目的构建表达人类白细胞抗原-E（HLA-E）基因慢病毒载体，探讨慢病毒介导HLA-E基因在肿瘤免疫中的意义。

Objective To construct the lentiviral expression vector of HLA-E gene and investigate its significance for further study on tumor immunity .

我国的EIAV弱毒疫苗是迄今为止世界上唯一投入应用的慢病毒疫苗，成功地控制了我国EIA的流行。

Donkey leukocyte attenuated equine infectious anemia virus ( DLA EIAV ) vaccine was the only successful lentivirus vaccine prepared in China , which has been applied extensively in China against EIA for twenty years .

为了研究JEVE蛋白与病毒受体互作功能域、宿主嗜性以及抗病毒药物，本研究利用了HIV-1型慢病毒载体系统构建JEVE基因假病毒。

To study the functional domain of the E protein which interacted with cell receptor , host tropism , and antiviral drugs , pseudotyped virus based on JEV E gene were constructed with human immunodeficiency virus type 1 ( HIV-1 ) vector system .

本实验观察大鼠鞘内注射PKCγ短发夹干扰RNA慢病毒对PKCγ和NMDA受体基因表达及对急性和慢性吗啡耐受的影响，从而探索吗啡耐受基因治疗的新策略。

This study observed effects of intrathecal injection of PKC γ short hairpin RNA lentivirus on expressions of PKC γ and NMDA receptor and on acute and chronic morphine tolerance in rats , in order to explore new strategies of gene therapy of morphine tolerance .