转录后基因沉默

RNA介导的转录后基因沉默

RNA-mediated Post-transcriptional Gene Silencing

RNA干涉（RNAi）是双链RNA介导的转录后基因沉默作用的重要机制之一。

Double-stranded RNA-mediated RNA interference ( RNAi ) is one of the process of sequence-specific post-transcriptional gene silencing .

病原介导的抗病性是一种RNA介导的抗病性，也是一种转录后基因沉默。

RNA-mediated virus resistance is known as pathogen-derived resistance ( PDR ) and is one kind of posttranscriptional gene silencing ( PTGS ) .

RNAi又叫转录后基因沉默。

RNAi is called posttranscriptional gene silencing .

转录后基因沉默（PTGS）普遍存在于生物界，如植物的共抑制、源于病原的RNA介导的病毒抗性、真菌的基因沉默和动物的RNA干扰等。

Post transcriptional gene silencing ( PTGS ) has been found to occur in plants , fungi and animals .

近年来，双链RNA介导的转录后基因沉默技术在油料作物脂肪酸组成的改良方面的研究获得了突破性进展。

Double strands RNA produced post transcriptional gene silencing ( PTGS ) technique is being used to alter the relative proportion of the major fatty acids present in oil plants .

RNA干扰（RNAinterference，RNAi）是一种序列特异性、转录后基因沉默的机制，目前，RNAi已应用于基因功能、疾病治疗的研究。

RNA interference ( RNAi ) is a sequence-specific , post-transcriptional gene silencing mechanism . Now RNAi has been adopted as a functional genomics tool and a gene therapy approach .

dsRNA介导的转录后基因沉默在真核生物中是普遍存在并且非常保守的一种降解靶标基因的机制。

Double-stranded ( ds ) RNA mediated gene silencing , a post-transcriptional and highly conserved process in eukaryotes , results in specific gene silencing through degradation of the target mRNA .

由于RNAi作用于RNA水平，故又称转录后基因沉默（PTGS）。

Because the effect of RNAi ison the level of mRNA , RNAi is a kind of PTGS ( post-transcription gene silencing ) .

转录后基因沉默（PTGS）及其在作物遗传改良中的应用

Post-transcriptional Gene Silencing ( PTGS ) and Its Application to Crop Genetic Improvement

植物中的转录后基因沉默（PTGS）是RNA沉默的一种形式，通过PTGS能对目标RNA进行特异性降解。

Posttranscriptional gene silencing ( PTGS ) in plants is a form of RNA silencing by which target RNA is degraded in a sequence-specific manner .

RNAi是双链RNA介导的转录后基因沉默的过程，是一种高效的高特异性抑制基因表达的新途径。

RNA interference ( RNAi ) is a newly described biological technology as genetic inhibitors of gene expression . RNAi is a process of sequence-specific posttranscriptional gene silencing mediated by double-stranded RNA .

RNA干扰（RNAi）是指生物体内由小干扰RNA（siRNAs）介导的具有高度特异性，只降解与之序列相应的单个内源基因mRNA的，在进化上高度保守的转录后基因沉默现象。

RNA interference ( RNAi ), an evolutionary highly conserved process of posttranscriptional gene silencing , can be triggered by small interfering RNAs ( siRNAs ) that mediate sequence-specific mRNA degradation .

这种转录后基因沉默机制被称为RNA干扰（RNAinterference，RNAi），哺乳动物细胞中RNAi作用越来越引起人们重视，并成为新颖的，重要的基因功能研究手段。

Such post-transcriptional gene silencing was known as the RNA interference . in the recent year , RNAi technology has been paid more attention in mammalian cell and become the new important method on the gene function .

马铃薯X病毒蛋白（P25）和黄瓜花叶病毒蛋白（CMV2b）作用于转录后基因沉默的信号阶段。

Both P25 of potato virus X and the cucumber mosaic virus 2b protein ( CMV2b ) target the signaling step of the pathway .

RNAi是通过双链RNA（dsRNA）介导的特异性地降解相应序列的mRNA，从而阻断相应基因表达的转录后基因沉默机制。

The mechanism of RNAi is through double chains ( dsRNA ) mediated specific degradation of targeted mRNA sequence , the transcription of targeted gene was inhibited due to the silence effect at the RNA level .

这种转录后基因沉默（PTGS）现象可以在植株体内系统传播，但不能遗传给后代。

Post-transcriptional gene silencing ( PTGS ) spread systemically in the first transgenic generation , but was not transmited to the offspring .

病毒载体诱导转录后基因沉默系统的建立及烟草（Nicotianabenthamiana）rbcs基因功能的研究

The Establishment of Virus Vector Induced Post Transcriptional Gene Silencing System and the Functional Analysis of rbcS gene in Tobacco , Nicotiana benthamiana

RNA干涉（RNAinterference，RNAi）是指同源双链RNA所引发的特异序列的转录后基因沉默现象。它被证明是一项能有效而特异地抑制基因表达的新技术。

RNAi ( RNA interference ) is a process of sequence-specific , post-transcriptional gene silencing in animals and plants initiated by double-stranded RNA that is homologous to the silenced gene and has proven as a novel approach to suppress gene expression specifically .

RNA干扰（RNAinterference，RNAi）是把双链RNA（double-strandedRNA，dsRNA）导入某些生物和细胞而引起同源mRNA降解的现象，是一种转录后基因沉默机制。

RNA interference ( RNAi ) is a phenomenon in which the introduction of double-stranded RNA ( dsRNA ) into certain organisms and cell types causes degradation of the homologous mRNA and it is a mechanism of post-transcriptional gene silencing .

对转录后基因沉默的特点和发生机制、PTGS与植物病毒抗性等方面进行综述，并在此基础上，就该技术策略应用于烟草抗病毒育种上的可行性进行探讨，并提出相关建议。

Features , mechanism , biological roles , and application of PTGS were reviewed and the feasibility of utilizing the mechanism to develop virus-resistant transgenic tobaccos was discussed .

RNA干扰（RNAinterference，RNAi）是真核生物中普遍存在的一种自然现象，是由双链RNA（double-strandedRNA，dsRNA）启动的序列特异的转录后基因沉默过程。

RNA interference ( RNAi ) as a natural phenomenon exists in almost any eukaryotic organisms . RNAi is the process of sequence-specific post-transcriptional gene silencing initiated by double-stranded RNA ( dsRNA ), which is enzymed into small interfering RNA ( siRNA ) by endonuclease .

RNA干扰（RNAi）是由小干扰RNA（siRNA）引发的生物细胞内同源基因的转录后基因沉默（PTGS）现象，是一种古老的生物抵抗外在感染的防御机制。

RNA interference ( RNAi ) is a kind of post-transcriptional gene silencing ( PTGS ) phenomenon initiated by small interfering RNA ( siRNA ) of homologous genes within bio-cell , it is an ancient defend mechanism of organism to resist external infection .

概述转录后基因沉默的机制及双链RNA、21～25nt小RNA和RNA依赖的RNA聚合酶在其中的重要作用，以及抑制PTGS的植物病毒抑制子方面的研究进展。

This paper reviews the mechanism of PTGS and the important roles of double-stranded RNA , 21 ~ 25nt small RNA and RNA-dependent RNA polymerase in it respectively . And the research progresses in PTGS suppression of plant viral suppressors are discussed .

目前研究发现黄瓜花叶病毒（CMV）的2b蛋白及马铃薯丫病毒（PVY）的HC蛋白等植物病毒蛋白能抑制植物转录后基因沉默，对它们相应的抑制机理已了解。

Present studies show that many different kinds of protein of plant virus which can inhibit the Post Transcriptional Gene Silencing of plants such as CMV-2b-pro and PVY-HC-pro etc have been found . The corresponding inhibitive mechanism of virus protein have been Understood .

RNA干扰（RNAi）是指生物体内利用具有同源性的双链RNA（dsRNA）诱发序列特异的转录后基因沉默（PTGS）的现象，它可以通过抑制蛋白表达模拟基因敲除技术。

RNA interference ( RNAi ) is a phenomenon in which double-stranded RNA ( dsRNA ) degrades its homological mRNA and leads to sequence specific post-transcriptional gene silencing ( PTGS ) in organism . RNAi can generate gene knockdown by inhibiting the expression of protein .

转录后基因沉默及植物病毒对它的抑制

Post-transcriptional Gene Silencing and It 's Suppression of Plant Virus

抑制转录后基因沉默的病毒蛋白及其抑制机制

Virus Inhibitory Protein of Post Transcriptional Gene Silencing and Its Inhibitory Mechanism

转基因植物转录后基因沉默机制及克服策略

Progress on the Post-transcriptional Gene Silencing Mechanism and Correspondent Strategy in Transgenic Plants

转录后基因沉默与植物的病毒抗性

Posttranscriptional Gene Silencing and Virus Resistance Against Unity