病毒载体
- 网络viral vector;Lentiviral vector;rAAV
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培养的A-T细胞经pTO-ATM病毒载体转导后,用免疫组织化学方法证明了ATM在这些细胞的异位表达。
Ectopic expression of ATM was demonstrated by immunohistochemistry in cultured A T cells after transduction of the pTO ATM viral vector .
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非病毒载体介导的人凝血因子Ⅷ基因在小鼠32D细胞系中的表达
Non Viral Vector Mediating Human Coagulation Factor ⅷ Gene Expression in Mouse 32D Cell Line
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E1/E3缺失型腺病毒载体引起细胞周期G2/M阻滞
E1 / E3 Deleted Adenovirus Vectors Induce Cell Cycle G_2 / M Arrest
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而基因修饰DC目前最常采用和最有效的方法为腺病毒载体。
At present , the most efficient method for genetic modification of DC is viral vectors .
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谷胱甘肽S-转移酶pi基因与逆转录病毒载体的重组体构建
Construction of Recombinant Glutathione S-transferase pi cDNA and Retrovirus Vector
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HBSAg及B(7-1)双表达逆转录病毒载体的构建及其在真核细胞中表达
Construction and expression in eukaryotic cells of HBsAg and B_ ( 7-1 ) coexpression retroviral vector
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逆转录病毒载体介导双靶区乙型肝炎病毒反义RNA模型的构建
Construction of recombinant retrovirus vector carrying dual target antisense RNA of hepatitis B virus
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目的建立逆转录病毒载体介导的人凝血因子Ⅷ(FⅧ)体外高效表达体系。
Objective To develop a retroviral mediated high efficient expression system of human coagulation factor ⅷ .
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含CD基因的腺病毒载体制备及体外抑瘤效果观察
Preparation of an adenovirus expressing cytosine deaminase and observation on its tumor killing effect in vitro
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目的构建人bcl-2重组腺病毒载体。
Objective To construct recombinant human Bcl-2 adenoviral vector .
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反义人类端粒酶RNA逆转录病毒载体构建及其对结直肠癌细胞的抑制作用
Construction of antisense human telomeric RNA retroviral vector and its inhibition on colorectal cancer cells
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目的:研究双靶区反义RNA的逆转录病毒载体的转染、表达及其抗乙肝病毒(HBV)的作用。
Objective : To study the effects of dual target antisense RNA against HBV infection .
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目的研究仙台病毒载体复制序列对未成熟树突状细胞(Dendriticcells,DC)蛋白质表达的影响,为联合运用二者进行基因治疗提供依据。
Objective To investigate the protein change in immature dendritic cells ( DC ) exposed to sendai virus replication sequence .
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晚期糖化终产物受体反义RNA腺相关病毒载体的构建及在肾脏系膜细胞中表达
Construction of recombinant RAGE antisense RNA adeno-associated virus vector and its expression in rat mesangial cell
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方法用逆转录病毒载体系统将αv、β5基因转染K562细胞,观察αv、β5亚基的表达;
Methods β 5 integrin cDNA was expressed in K562 cells through a retroviral vector system .
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用双拷贝逆转录病毒载体转导人G-CSFcDNA的实验研究
A study on improvement of expression of human G-CSF cDNA transferred with retroviral double copy vector
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本文主要介绍有可能用于基因工程疫苗的DNA及RNA病毒载体构建及其应用。
Here , we mainly discuss the construction and application of DNA , RNA vectors which can be potentially used for delivery vaccine .
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HIV-1重组病毒载体疫苗冻干制剂的制备及其联合免疫效果的研究
Prepare of Lyophilized Recombinant Virus-Based HIV-1 Vaccine and Prime-Boost Strategy
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本文采用非复制型脓病毒载体,利用同源重组方法,获得表达G蛋白多肽的非复制型重组腺病毒。
We used the replication - defective adenovirus vector and the homologous recombination , so we obtained the recombinant adenovirus by carrying the G-protein polypeptide .
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人B7-1基因的cDNA克隆及其重组腺病毒载体制备
Cloning of human B7-1 gene cDNA and preparation of adenovirus vector carrying B7-1
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目的构建人GM-CSF基因的重组腺病毒载体,探讨其在树突状细胞(DC)中的表达。
Objective To construct the recombinant adenovirus vectors containing human GM-CSF gene , and express in dentritic cells .
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一DXFD(52)相关人肝细胞cDNA的部分序列分析及其反转录病毒载体的构建
Partial Sequence Analysis and Construction of Recombinant Retroviral Vec-tor of a DXFD_ ( 52 ) - Related Human Hepatocyte CDNA
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HIV-1来源的慢病毒载体转染大鼠脊髓神经干细胞
Lentivirus vector deriving from human immunodeficiency virus-1 transfer to rat spinal cord neural stem cells
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腺病毒载体介导的PML生长抑制因子转染人角质形成细胞的实验研究
Experimental Study on the Keratinocytes Transfected by Adenovirus-mediated PML Growth Suppressor
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大鼠Nogo受体基因RNA干扰慢病毒载体的构建与鉴定
Construction and identification of a recombinant lentivirus harboring RNAi targeting rat Nogo receptor gene
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家蚕核多角体病毒载体稳定高效表达HBeAg条件及其初步应用的研究
Study of the High-Production and the Primary Application of the HBeAg Expressed with BmNPV System
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带有CMV、CEA启动子的腺病毒载体介导TK基因在肿瘤细胞中的表达特性
Characteristics of Adenovirus Vector Mediated TK-gene Expression Promoted by CMV and CEA Promoters in Tumor Cells
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用杆状病毒载体在家蚕细胞中表达HBeAg基因及其表达产物纯化的研究
Expression of HBeAg Gene with Baculovirus Vector in Silk Worm Cells and its Expression Product Purification
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用腺相关病毒载体介导人血管内皮生长因子受体KDR胞外区基因的表达
Expression of Extracellular Domain of VEGF Receptor KDR with Recombinant AAV
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假型病毒载体介导tPA基因转导乳鼠心肌成纤维细胞的实验研究
Pseudotyped Retroviral Vector-Mediated Transduction and Expression of Tissue-typed Plasminogen Activator Gene in Neonatal Rat Myocardial Fibroblasts