生物学方法

方法：细胞培养、逆转录-聚合酶链反应（RT-PCR）及放射免疫等细胞、分子生物学方法。

Methods : Cell culture , RT-PCR and radioimmunoassay were used .

以PCR为基础的分子生物学方法使分支杆菌感染的快速、准确诊断成为可能。

Molecular techniques based on PCR make it possible for rapid and accurate diagnosis of mycobacterial infection .

一是基于免培养的方法，包括显微镜观测法，荧光原位杂交技术（FISH），基于直接提取微生物总DNA的分子生物学方法等。

And non-culturable methods , which include viewing by microscope , FISH , new molecular biology method based on DNA extraction directly of microorganism , etc.

目的应用分子生物学方法判定经家系分析初步认定为X连锁遗传的高度近视家系的遗传方式。

Objective Using molecular biological method to determine the inherited modal of a family which was considered as X-linked high myopia by pedigree analysis .

目的：研究不同分子生物学方法检测乙型肝炎病毒（HBV）DNA的敏感性及影响因素。

Objective To study sensitivity and influence factor of the determination of hepatitis B virus ( HBV ) DNA by different methods of molecular biology .

miRNA靶标预测的系统生物学方法研究

Study on Systems Biology Methods of miRNA Target Prediction

结论：1.应用分子生物学方法可以成功地克隆大鼠GAP-43基因并构建以腺相关

Conclusion 1.Successful construction of the recombinant adeno - associated virus

目的利用PCR对近交系小鼠11个品系的20个微卫星基因座进行遗传检测，为实验室日常检测近交系小鼠的遗传背景提供一种分子生物学方法。

Objective To analyze the polymorphisms of 20 microsatellites in 11 strains of mice by PCR and to explore a new method for inbred strain mice genetic monitoring .

探索防治PCO的分子生物学方法。

Look for a new molecular biology method to prevent PCO .

目前致突变菌株（mutatorstrain）的使用是蛋白质定向进化中所采用的一种体内分子生物学方法。

Nowadays the application of mutator strain is a in vivo method in molecular biology for directed evolution of proteins .

用生物学方法研究了河南烟草马铃薯Y病毒（PVY）的体外特性。

The vitro characteristics of PVY in Henan Province were studied by biological methods .

方法：利用PCR、PCRSSCP等分子生物学方法，检测Ecadherin基因第5、7对外显子在喉癌组织中的基因突变。

Method : Exploration of exon 5 and exon 7 of the E cadherin gene by polymerase chain reaction ( PCR ), SSCP in the laryngeal cancer .

本论文运用生物信息学和分子生物学方法，从日本七鳃鳗白细胞cDNA文库中搜索到一条能够表达Vav3的EST序列。

In this study , molecular biology and bioinformatics methods were used to analyze the expressed sequence tags ( ESTs ) of the leukocytes cDNA library of Japanese lampreys .

目的以分子生物学方法检测药物干预对NAFLD大鼠基因表达的影响。

Objective To detect the gene expression of NAFLD rats in the medical intervention using the methods of molecular biology .

微生物生态学中分子生物学方法及T-RFLP技术研究

Molecular Approaches Studying on Microbial Ecology and T-RFLP Technique

比较两种分子生物学方法的结果表明，PCRTGGE技术比PCRRFLP技术更能精确地反映土壤微生物的动态变化，并且能同时快速地对多个样品进行有效区分。

It also manifested that PCR-TGGE is superior to PCR-RFLP for detail dynamics of soil microbial community , and it is a rapid method to discriminate multiple samples simultaneously .

结论：用分子生物学方法进一步证明革螨、恙螨体内存在HFRSV。

It demonstrated the existence of HFRSV in gamasid mite and chigger mite by molecular biology furtherly .

尽管，通过遗传学、生物化学、细胞生物学方法，ABA结合蛋白的研究取得了巨大的进展。但是，遗憾的是至今仍然还未鉴定出任何可能的ABA受体。

The greatest progress has been made using genetic , biochemical and cell biological approaches to study ABA binding proteins , however , no ABA putative receptors have been identified to date .

方法采用标准生物学方法即TGFβ1对水貂肺上皮细胞生长抑制的方法及放免法分别检测72例肝硬化患者及24例正常人血清TGFβ1及血浆ET-1水平。

Methods Peripheral blood samples were obtained from 72 patients and 24 normal controls . The blood levels of TGF β 1 and ET 1 were measured by using a standard bioassay and the radioimmunoassay .

因此，通过药物或分子生物学方法抑制IκBα降解进而阻止NF-κB活化已成为目前研究NF-κB作用的一种重要方法。

Therefore , the inhibition of the degradation of I κ B α and the prevention of the activation of NF - κ B with drugs or molecular biological methods has become an important approach in study of the effect of NF - κ B at present .

目的比较RAPD、STR方法与生化标记方法对近交系小鼠检测的差异，为实验室保证近交系动物遗传质量提供一种分子生物学方法。

Objective Comparing RAPD map and STR map with the result of biochemistry method in monitoring inbred mouse BABL / c in order to study one new method for monitoring the quality of laboratory animals .

绵羊肺炎支原体（MO）是引起绵羊传染性胸膜肺炎的病原体，本研究采用常规细菌学方法及分子生物学方法，对石河子公园野生盘羊发生疑似传染性胸膜肺炎病例进行了病原学诊断。

Mycoplasma ovipneumoniae ( MO ) is the pathogen caused the sheep infectious peripneumonitis , this study adopted conventional bacteriological methods and molecular biology methods to diagnose the etiologic of wild Argali of shihezi park that suspected infectious peripneumonitis Mycoplasma .

应用PCR-DGGE分子生物学方法对东江水源工程沿途水样中的微生物群落结构的动态变化以及种群多样性进行了研究。

The dynamic metamorphose , composition structure and diversity of microbial community in the raw water along the Dongjiang River were studied by molecular biology PCR-DGGE assessment .

应用分子生物学方法检测32例膀胱移行上皮癌中P53、K－ras、HPV基因，发现有34.4%的膀胱癌P53基因突变，21.9%膀胱癌K－ras基因突变，6.3%膀胱癌HPV阳性。

Cases of transitional cell carcinoma of bladder were detected for P53 , K-ras genes and HPV using molecular biologic techniques . P53 mutation was observed in 34.4 % , K-ras mutation in 21.9 % and HPV-positive in 6.3 % .

此外，购买了花粉表面蛋白质T-DNA插入突变体，通过分子生物学方法进行了T-DNA插入鉴定以及插入位点鉴定，并获得了96个突变体株系的纯合植株。

Besides , T-DNA insertion mutant lines of pollen surface proteins have been purchased , and through T-DNA insertion and locus analysis , 96 homozygous mutant lines have been acquired .

方法收集非典型肺炎患者的各种临床标本，用狗肾传代细胞（MDCK）进行病原体分离，并运用血清学和分子生物学方法进行鉴定。

Methods Pathogens were isolated from variety of samples collected from atypical pneumonia patient by using MDCK cells , and identified with serological and molecular methods .

目的探讨用分子生物学方法检测血液Duffy血型基因型及该方法应用于HDN产前检查的可行性。

Objective To evaluate a new molecular method for Duffy blood group genotyping and the possibility to apply this method to prenatal diagnosis of HDN .

常规检测AFB1的方法有生物学方法、HPLC及薄层层析等方法；由于各方法的局限性，人们逐渐专注于应用免疫学方法进行检测。

Though there are some conventional detection methods such as biological method , HPLC and TLC , due to the limitations of each method , application of immunological method is gradually attracting researchers ' interest .

方法：采用生物学方法研究LMWH对卵白蛋白（OA）致敏豚鼠平滑肌的收缩及肺组织液中白三烯（LTs）及NO的影响。

Methods : The effect of LMWH on smooth muscle , leukotrienes ( LTs ) and NO from pulmonary tissue of guinea pigs sensitized by ovalbumin ( OA ) was studied by biological methods .

分别于移植后第1天、第7天、第21天后取远端结肠组织，经分子生物学方法检测Y染色体的性别决定区段（sry）作为标志，以确定MSCs的肠道定位情况。

The sex-determining gene ( sry ) located on Y chromosome was examined by PCR in colon of female rats on days 1,7 and 21 after transplantation , respectively , to localize the colon MSCs .