强启动子

结论：强启动子系统可作为可溶性抗体Fab的表达载体，T7启动子在可溶性抗体Fab表达中作用比Lac启动子强。

Couclusion : Strong promotor system can be used to express soluable antibody Fab . The role of T7 promotor in expression of soluble anti-Fab antibody is stronger than Lac promotor .

强启动子枯草芽孢杆菌核黄素操纵子整合型载体的构建并在枯草芽孢杆菌中的表达

Integration Plasmid Construction of Strong Promotor Riboflavin Operon and Expression in Bacillus Subtilis

目的：探索强启动子T7在可溶性抗体Fab表达中的作用。

Objective : In order to study the function of T7 promotor in expression of Fab .

分别以CaMV35S强启动子和Ac转座酶自身携带的弱启动子启动，以GFP、Lc、GUS基因作报告基因，构建了7个激活表达标签载体。

Seven activation tagging constructs were built promoted with CaMV 35S and Ac self-promoter , and with GFP , Lc , GUS as reporter genes .

三种强启动子和egt基因缺陷在重组核型多角体病毒杀虫剂研发中的意义

The sense of three strong promoters and egt-minus in research and development of recombinant NPV pesticides

因此可以进一步证实，citrin基因启动子是一种具有种子特异性表达功能的强启动子。

The above data suggested that the citrin gene promoter was a high active seed-specific promoter . 5 .

在启动子上游存在GATA-1结合位点，构成GATA-1基因的强启动子，这种强启动子是为充分活化及与体内自身红系核蛋白结合所必需。

There are GATA-1 binding sites at upstream of promoters , which constitutes a strong GATA-1 gene promoter . This strong promoter is necessary for getting fully activation and combining with its own erythroid nuclear protein .

另外我们对枯草芽孢杆菌中的核黄素操纵子做了进一步的优化和改造，主要包括：调控序列的去除、外源强启动子spol的加入、原启动子的去除以及四环素抗性基因的加入等。

On the other hand the rib operon of the Bacillus subtilis was also modified : eliminating the rfn box , replacing the rib promoter by constructive bacteriophage promoter spol and taking the tetracycline resistance gene as a selectable marker etc.

利用强启动子启动后续基因的转录，可极大地促进目的基因的表达。

Using strong promoters may facilitate transcription of the target gene significantly .

鸡痘病毒载体强启动子的构建和筛选

Construction and selection of a strong promoter for fowl pox virus based vectors

强启动子能提高外源基因的转化频率，从而提高外源基因的表达量。

The strong promoter can increase the transcription frequency and increase foreign genes expression level .

初步证实分离的互补链基因启动子可作为新型强启动子应用于双子叶植物尤其是棉花的遗传转化。

The experiments suggested that isolated bidirectional promoter , as a novel strong promoter , could be used for dicots and especially cotton genetic transformation .

外源淀粉水解酶能在酵母中高效表达并分泌，其主要因素在于：在构建载体时组入强启动子；

The main factors for high efficiency expression and secretion of exogenous amylum hydrolase in S.cerevisiae are as follows : involvement of strong promoter in carrier construction ;

但与商业强启动子相比，复合启动子活性要弱一些或相当。

But compared with the commercial strong promoters , the transcriptional activity of the composed promoter were less than as or the same as that of the strong promoters .

结果表明，获得的强启动子比原来的提高了3～8倍，而弱启动子则活性下降明显，其中3个几乎无活性。

Results showed that the activity of strong promoters had been improved 3 ~ 8 fold while one weak promoter had been decreased 3 fold and three weak promoters showed almost no activity .

二构建了用强PL启动子控制的phoA信号肽引导的重组分泌表达体系。

Secondly , strong PL promoter and phoA signal peptide were used to construct secretory expression system .

研究表明，采用强PL启动子可提高分泌表达的水平，并且在phoA信号肽引导下，重组产物准确被加工后进入培养基。

Our result showed that strong PL promoter could be used to increase the expression level of glucagon and phoA signal peptide could direct recombinant glucagon into culture medium .

利用强的组成型启动子虽能提高RNAi效率，但是外源基因的组成性表达对转基因家蚕的发育会造成一定的影响。

Using a strong constitutive promoter could increase the efficiency of RNAi , but the constitutive expression of the foreign gene may affect the development of transgenic silkworm .