单顺反子
dān shùn fǎn zǐ
- monocistron
单顺反子-
单顺反子表达人GM-CSF和B7.1融合基因真核表达载体的构建和鉴定
Construction and Identification of Eukaryotic Vector Expression Containing Human GM-CSF and B7.1 Fusion Gene from A Single Cistron
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利用重叠延伸PCR法成功构建pIRES单启动子双顺反子嵌合抗体表达载体，并在哺乳动物表达体系（CHO细胞）中表达嵌合抗体。
By overlapping extension polymerase chain reaction , we successfully construct pIRES single promoter bicistronic chimeric antibody expression vector and express chimeric antibody in CHO mammalian expression system .