开放阅读框

结果表明，TK基因的开放阅读框（ORF）和所比较的各株的核苷酸和氨基酸同源性都在99%以上。

The result showed that the homology of the nucleotide sequence and amino acid sequence of JA TK ORF with other strains was over 99 % .

RTK有一个完整的开放阅读框，其编码的蛋白质具有信号肽和跨膜结构域，可能是一个具有磷酸化功能的跨膜蛋白。

RTK has a perfect ORF that code proteins with signal-peptide and transmembrane region . It is predicted that the product of RTK may be a transmembrane protein with phosphorylation site .

每个型的cDNA都包括一个编码131个氨基酸的开放阅读框。

Each mango profilin cDNA includes an open reading frame coding for 131 amino acids .

结果表明林麝IL-2开放阅读框全长468bp，编码155个氨基酸。

It showed that the length of M.berezovskii IL-2 was 468 bp , coding 155 amino acids .

断裂蛋白质内含子是指蛋白质内含子被分裂成两部分即N端区域（IN）和C端区域（IC），分别由不同的开放阅读框表达。

An intein can split into two pieces as N-fragment and C-fragment of intein ( IN and Ic ), and express by two different open reading frame .

所得cDNA序列全长1195bp且包含一个完整的开放阅读框，编码294个氨基酸。

The cDNA fragment was 1 195 bp with an entire open reading frame ( ORF ), encoding 294 amino acids .

711nt的开放阅读框编码236个氨基酸残基的猪CD8α前体蛋白（含1个N-糖基化位点）。

The open reading frame encoded a protein precursor with 236 amino acids residues and one N-glycosylation site .

接着为一个1323nt的开放阅读框（ORF）；

Open reading frame ( ORF ) is of 1323 nt .

2个测序片段均包含开放阅读框（ORF）序列。

Both of them included an open reading frame ( ORF ) .

TACE是多结构域的I型跨膜蛋白，开放阅读框编码824个氨基酸。

TACE is a multi-domain , type I transmembrane protein , in which opening reading frame encodes 824 amino acids .

ORF开放阅读框分析表明，开放阅读框长399bp（54-452bp）编码132个氨基酸。

The open reading frame analysis includes 399 bp ( 54-452 bp ) , encoding 132 amino acids .

通过RT-PCR及cDNA末端扩增（RapidAmplificationofcDNAends，RACE），获取△~4-脂肪酸脱饱和酶cDNA的全长基因，序列分析显示其开放阅读框由1560个核苷酸组成，共编码519个氨基酸；

And the full-length cDNA of delta-4 fatty acid desaturase was obtained by RT-PCR and RACE ( rapid amplification of cDNA ends ), its whole ORF is 1560 bp encoding 519 amino acids ;

正链RNA只含一个长的开放阅读框，编码一个由1146个氨基酸组成的蛋白，推测其分子量为131.8kD。

Computer analysis indicated tha the coding strand contained only one open reading frame ( ORF ) encoding for a polypeptide of 1146 amino acids with predicted molecular mass 131 . 8 kD .

报道逆转录－多聚酶链式反应（RT－PCR）联合一步程序，扩增大于1kb的拟南芥菜G蛋白α亚基基因的开放阅读框。

It is reported that a one-step reverse transcription and polymerase chain reaction ( RT-PCR ) procedure for amplification of open reading frame of G-protein α subunit gene from Arabidopsis thaliana which is larger than 1 kb .

在对虾白斑综合征病毒（Whitespotsyndromevirus，WSSV）的基因组中发现一个具有细胞因子受体特征的开放阅读框，该阅读框全长2022个核苷酸，编码674个氨基酸，蛋白质理论分子量为76kDa。

One ORF of White spot syndrome virus ( WSSV ) genome has homology with the cytokine receptor . This ORF contains 2022 nucleotides and codes for 674 amino acids with a theoretical molecular weight of 76 kDa .

HCV基因组仅有一个开放阅读框，编码一个由3010到3033个氨基酸组成的多聚蛋白，通过细胞和病毒的蛋白酶切割，形成结构蛋白和非结构蛋白。

HCV genome only has a long open-reading frame , encoding a polyprotein of about 3010-3033 amino acids that is processed by a combination of host and viral proteases into structural and non-structural protein .

全长7956bp，含有一个7761bp的开放阅读框（ORF），编码2587个氨基酸残基。

This cDNA sequence is 7956 bp in length , containing a 7761 bp open reading frame , which encodes 2587 amino acid residues .

本研究从人的睾丸组织cDNA文库中分离出一条新cDNA，全长1935bp，其中含有一个1605bp的开放阅读框，它编码了535个氨基酸。

We have isolated a novel full-length cDNA from cDNA library of human test is . This cDNA of 1935 bp contains an 1605 bp open reading frame ( ORF ) encoding 535 amino acids ( aa ) .

其序列与E.maximaHoughton株的开放阅读框一致。

The ORF sequences of the gene was the same as that cloned from E.maxima Houghton strain .

PVX的所有开放阅读框处于负向（纯化）选择，但是依赖于RNA的RNA聚合酶基因（RdRp）中间区域处于正向（多样化）选择。

All the open reading frames of PVX were under negative ( or purifying ) selection , but the central region of RdRp was under positive or diversifying selection .

除此，研究者还公布了突变的定位已便知晓RNA的功能原件，例如核糖体结合位点（IREs）和上游开放阅读框。

On top of these studies , the authors now show that the variants map to known RNA functional elements , such as internal ribosome entry sites ( IREs ) and upstream ORFs .

目的对扩增的墨西哥利什曼原虫环形DNA1（CD1）部分片段进行测序，确定具有蛋白编码功能的开放阅读框。

Objective To determine the nucleotide sequence of cloned CD1 fragments from Leishmania mexicana and find ORFs predicted to have protein coding function .

该cDNA全长约为0.9kb，含有一个516个核苷酸的完整开放阅读框，编码172个氨基酸，等电点为8.57，分子量18.2KDa。

The full-length cDNA was about 0.9 kb , which contained an open reading frame ( ORF ) of 516 bp and coded for 172 amino acid residues with isoelectric point of 8 . 57 and molecular weight of 18 . 2 kD .

采用RACE-PCR的方法扩增到草鱼IgM重链的cDNA全长，其开放阅读框包含1731个核苷酸，编码576个氨基酸。

The IgM cDNA full sequence of grass carp has been cloned using RACE-PCR and PCR . Its open reading frame has 1731 nucleotides which encodes a 576 amino acid peptide .

序列分析发现拼接的cDNA含有一个411bp的开放阅读框，编码136个氨基酸，ATG为起始密码子、TAA为终止密码子。

Sequence analysis revealed that splicing of the cDNA contained a 411 bp open reading frame , encoded 136 amino acids , and ATG was the initiation codon and TAA was the stop codon .

这一研究结果表明调控MAC1基因转录的启动子是位于MAC1基因的开放阅读框之内，这是一个有意义的新发现。

This unexpected result indicates that the MAC1 promoter is likely located within MAC1 open reading frame .

另外通过对WSSV全序列的分析，找到数个可能的编码囊膜蛋白的开放阅读框（ORF），分别对其进行展示，分析其对宿主细胞的结合活性。

Furthermore , by screening the WSSV genome , we selected several putative ORFs that may code envelop protein and phage display to analyze their binding ability to host cell .

条带DTA全长259bp，尽管未发现有同源序列和新的开放阅读框，但它可作为探针从cDNA文库中筛选全基因序列，进而寻找与细胞质雄性不育有关的开放阅读框架。

Fragment DTA was composed of 259 base pairs , though no sequence homology and no open reading frame was found in the sequence . It could be used as a probe to clone the whole length gene from the cDNA library .

所有HERV亚家族中，HERVK被发现是最有致病潜力的，HERVK具有活跃的ENV和Gag蛋白的开放阅读框（ORF）。

Among all sub families of HERV , HERV K is found to be most potent one with active open reading frame ( ORF ) of env and gag protein .

所获得的4-香豆酸：CoA连接酶（4CL）基因长1911bp，开放阅读框1623bp，编码540个氨基酸。

The size of the cDNA fragment is 1911 bp , which included an 1623 bp open reading frame and encoded a peptide of 540 amino acids .