结构基因

大肠杆菌野生株K（99）菌毛蛋白结构基因的克隆与序列分析

Structure Gene Cloning and Sequence Analysis of K_ ( 99 ) Pili Protein of ETEC from Wild Strains

采用PCR扩增方法获取P1结构基因。

The P1 structure gene was amplified by PCR method .

3株致病性鸡大肠杆菌P型菌毛结构基因的克隆、序列测定及同源性分析

Cloning and Sequence Analysis of Type P Pilin Structural Gene in Pathogenic Escherichia coli from Chichen

转移RNA结构基因共有序列的统计分析

Statistical Analysis of Conserved Sites in the Structural Genes of tRNA

鸡大肠杆菌P型菌毛结构基因（papA）的克隆与序列测定

Cloning and Nucleotide Sequencing of papA Gene from Avian Escherichia coli Strain

核衣壳蛋白基因（N基因）传染性支气管炎病毒的重要结构基因。

Nucleocapsid ( N ) gene is one of the major structural genes of infectious bronchitis virus ( IBV ) .

用连续长片段RT-PCR扩增HCV结构基因

Amplification of HCV structure genes by continuous long RT-PCR

目的构建和表达旋毛虫肌幼虫编码相对分子质量（Mr）31000抗原结构基因（TspE1）的重组质粒。

Objective To construct and express recombinant plasmid containing the structural gene encoding { Mr 31 000 } antigen of Trichinella spiralis muscle larvae .

牛CD14结构基因的PCR扩增及序列分析

PCR amplification and Sequencing of the structural gene encoding bovine CD14 antigen

结论：构建的3种腺病毒穿梭质粒分别可以表达HCV不同段的结构基因，为包装表达HCV不同结构基因的腺病毒载体奠定了基础。

This should be useful to pack adenovirus expression vector which can express HCV structure genes .

目的与方法：使用连续长片段RT-PCR，扩增HCV的结构基因。

Objectives and Methods : The structure genes of HCV was amplified with continuous long RT PCR .

目的克隆和表达人免疫缺陷病毒（HIV）Ⅰ型中国株E、B亚型代表株的结构基因gag。

Objective To clone and express subtype E and subtype B gag gene of the prevalent HIV 1 strain in China .

SarA是一种DNA结合蛋白，可以激活ica操纵子中结构基因的表达。

SarA , a DNA-binding protein encoded by sarA gene , activates the expression of icaA in S.aureus .

利用荧光定量PCR技术分析了与花色苷生物合成相关的14个结构基因和2个调节基因在植物生长调节剂处理下的mRNA转录水平。

Real-time quantitative PCR was used to study the effects on the mRNA transcriptional level of 14 structure related genes of anthocyanin biosynthesis and 2 regulating genes .

本实验是在已获得TBa结构基因的基础上，进行TBa的原核表达。

The objective of the experiment is to express the TBa in the prokaryotic system further .

目的了解戊型肝炎病毒的变异。方法用双脱氧法对亚洲主要戊型肝炎流行国家的戊型肝炎病毒结构基因区段进行了分析，并根据cDNA序列确定了其氨基酸的序列。

Dideoxy chain termination method was applied to sequence hepatitis E virus structure gene of Asia isolates , cDNA sequence and amino acid residues were determined .

目的：获取旋毛虫ES抗原的结构基因，对其鉴定和克隆，并研制旋毛虫基因重组抗原。

AIM : To identify and clone structural genes encoding ES antigen from T. spiralis for preparing gene recombinant antigen of T. spiralis .

结论：获得了含有肺炎支原体P1蛋白结构基因的克隆菌株。

Conclusion : We obtained the recombinant clone strain that contain P1 protein structure gene of mycoplasma pneumoniae .

HCV非结构基因NS5分子生物学研究进展

Progress of Molecular Biological Research on Nonstructural Gene NS 5 of Hepatitis C Virus

乙型肝炎病毒（HBV）基因组为部分双链环状DNA病毒，属嗜肝DNA病毒科，其基因组约3200个碱基对（bp），分为结构基因和调节基因序列两部分。

Hepatitis B virus ( HBV ) genome is partial double strands and looped DNA virus , which have 3200 bps , consisting of two parts of structure and regulator gene ranks .

引起免疫失败的IBV分离株的生物学特性及结构基因分析

Analysis of Biological Characteristics and Structural Genes of IBV Isolate from Chickens with Immunoprophylaxis Defeat

旋毛虫肌幼虫ES抗原特异性蛋白2个结构基因的分子克隆及其高效表达

Cloning of Two Structural Genes Encoding Specific Proteins in ES Antigen from Trichinella spiralis Muscle Larvae and Their High Level Expression in E.coli and Yeast

旋毛虫ES抗原结构基因TSPGⅡ重组表达质粒构建

Construction of Recombinant Expression Plasmids of Structural Gene TSPG ⅱ Encoding ES Antigen from Trichinella Spiralis

这些结构基因的遗传特征表明它们适合用来构建病毒样颗粒疫苗载体和E亚型的亚单位疫苗表达载体。

The genetic characteristics of the two structural genes indicated that they could be used to construct VLPs ( Virus-like Particles ) vector of Subtype A and expression vector of subunit vaccine of Subtype E.2 .

2a型丙型肝炎病毒非结构基因5区（NS5）全长克隆的构建

Amplification and construction of the vector containing full-length nonstructure gene 5 of hepatitis C virus

COⅡ、ATPase8、ATPase6三个结构基因不同家蚕/野桑蚕之间的比对分析表明，它们在核苷酸水平、氨基酸水平的同源性很高。

Aligment analysis of genes of CO ⅱ, ATPase 8 and ATPase 6 respectively showed that there are high homology on the level of nucleotide acid and amino acid among different silkworm / wild silkworm .

比较cDNA序列与结构基因序列来分析两个候选基因的结构，同时分析了不同来源广亲和材料的候选基因序列。

The structures of two candidate genes were revealed by comparing their cDNA sequences with genomic sequences . Meanwhile , the sequences of candidate genes from different WCVs were also investigated .

根据已获得的序列设计特异性引物，利用3'-RACE方法并结合巢式PCR扩增该基因的3'端部分序列，获得了甜荞麦蛋白酶抑制剂基因的3'端，加之先前获得的部分结构基因序列，总长为385bp。

To get the full gene sequence of BPI , part of 3 ' - end gene sequence was also cloned with particularly designed primers by 3 ' - RACE PCR combined with nested PCR .

结果初步确定了大肠杆菌染色体上的lac操纵子结构基因位点适合外源基因的敲入和表达。

So we assured that the sites of structure genes in the lac operon of Escherichia coli chromosome were suitable for expressing foreign genes .

在TK结构基因中插入了编码痘苗病毒7.5K蛋白基因的启动基因（P（7.5）），它可以有效地启动外源基因在痘苗病毒中表达。

The promoter of the gene coding for vaccinia viral 7.5 K protein ( P_ ( 7.5 )) was inserted into the TK structure gene .