负链

原位杂交法证实细胞内存在负链RNA，也位于胞浆中。

The minus strand of HCV RNA was located within cytoplasm .

在同例肝（癌）组织中，若负链HCVRNA阳性，则其信号强度与正链基因无明显差别；

In the cancer tissues of a same case , if minus strand RNA of HCV was positive , its signal intension had no significant difference from that of HCV plus strand RNA .

负链RNA病毒的反向遗传技术

Reverse Genetics of Negative-strand RNA Viruses CHAIN OF TESTS OF SERIES

原位杂交法证实细胞胞浆内存在负链RNA。

The HCV RNA were detected in cytoplasm by in situ hybridization .

从带烟草花叶病毒外壳蛋白亚基因组启动子的负链RNA产生正链RNA

Positive-strand produced from negative-strand RNA containing coat protein subgenomic promoter of tobacco mosaic virus

A型流感病毒基因组含有8个单股负链RNA病毒节段，从禽类中分离到15个血凝素、9个神经氨酸酶亚型。

Influenza A viruses contains a genome of eight single-stranded RNA segments of negative-polarity .

负链RNA病毒作为活病毒载体具有巨大的优势。

The negative RNA virus has many extremely attractive advantages to be the expression vector .

但其反向遗传学的发展则远远落后于正链或负链RNA病毒。

However , reverse genetics of dsRNA viruses lag behind the positive-strand or minus-strand RNA viruses .

负链RNA病毒的RNA聚合酶的低保真性是病毒突变最主要的原因。

The low fidelity of the RNA polymerase of negative-strand RNA viruses is the major reason for virus mutation .

TTV为单负链DNA病毒，目前的研究表明TTV在感染者体内可能存在两种形状的基因组，一种是线状，一种是环状，多数研究表明为后一种。

TTV is a negative strand DNA virus .

抗HCV阳性患者体液中正链和负链HCVRNA的检测及意义

Detection and significance of plus-and minus HCV RNA in body fluids of anti-HCV positive patients chain of Immunology

肝炎病人血清外周血单核细胞及肝脏中GBV-C/HGV负链RNA的检测

Detection of minus-stranded GBV-C / HGV RNA in the serum , liver and peripheral blood mononuclear cells in patients with hepatitis

分别建立正链和负链HCVRNA的巢式荧光定量RT-PCR方法并分析敏感性和特异性。

A nested fluorescence quantitative RT-PCR method to detect positive - and negative - strands HCV RNA is established .

负链RNA作为病毒复制活动的直接标志，对评估和预测丙肝治疗的疗效有一定的意义。

It suggests that detection of negative strand RNA of HCV may be a useful marker for evaluating and predicting the effect of treatment .

而在9份PBMC和15份肝脏标本中检出HGV负链RNA。

Minus-strand HCV RNA was found in 9 cases in PBMC and 15 cases in liver sample .

4例患者中，1例患者细胞中连续2个月检测到HCVRNA正链，HCVRNA负链间断出现。

HCV RNA plus strain was detected in one patient 's PBMC continuously for 2 months , and minus strain was detected intermittently .

禽流感病毒（AIV）是一种单股负链RNA病毒，属正粘病毒科流感病毒属的一个员。

Avian influenza viruses ( AIV ) are segmented , negative-sense , single-strand RNA viruses of the family Orthomyxoviridae .

博尔纳病病毒（BDV）是单股负链RNA病毒，在体内和体外都缺乏细胞致病性。

The single-stranded RNA Borna disease virus ( BDV ), lacks cytopathogenicity both in vitro and in vivo .

通过对外周血单个核细胞（PBMC）中正链及负链HCVRNA的检测来探讨HCV在PBMC中的复制与丙肝慢性化及复发的关系。

To study the relation of chronic hepatitis C and relapse with HCV RNA in sera and peripheral blood mononuclear cells ( PBMC ) .

犬细小病毒（CPV）为无囊膜的单股负链DNA病毒，是引起犬急性出血性胃肠炎和幼犬心肌炎的主要病原。

Canine parvovirus ( CPV ) is non-enveloped single stranded negative DNA virus , is a major pathogen of dogs with acute hemorrhagic gastroenteritis and puppies myocarditis .

猪圆环病毒2型（porcinecircovirustype2，PCV2）为无囊膜的单股负链DNA病毒，属圆环病毒科（Circoviridae）成员。

Porcine circovirus type 2 ( PCV2 ) is a small non-enveloped single-stranded circular DNA virus classified in the Circoviridae family .

研究背景呼吸道合胞病毒（RSV）为副粘病毒科肺炎病毒属、有包膜、非节段性的单负链RNA病毒。

BACKGROUND As one of the members of the Paramyxoviridae family , Respiratory syncytial virus ( RSV ) is an enveloped , non-segmented , negative-strand RNA virus .

AIV的基因组由8条单股负链RNA组成，NS基因是流感病毒的第8节段基因。

The genome of Avian Influenza Virus ( AIV ) consists of eight single-stranded , negative sense , segmented RNAs in which the NS gene is smallest .

将以上两种RNA转录物混合并经37℃保温后，检测结果表明所得核酶RNA对PLRV-Ch复制酶基因负链RNA在体外具有较强地特异切割活性。

After mixture of the RNA transcripts and incubation at 37 ℃, results showed that the ribozyme has highly catalytic activity to the PLRV-Ch negative RNA in vitro .

仅4例患者唾液中负链HCVRNA检测结果阳性，而所有受检精液和阴道分泌物中负链HCVRNA均为阴性，提示部分患者唾液腺中可能存HCV复制中间体。

Only 4 patients were minus HCV RNA positive in their saliva , but none in the sperm or vaginal discharge . This meant that HCV probably replicated in saliva glands as reported previously .

以HCV正、负链RNA3′末端的序列为模板，体外研究NS5B蛋白催化的RNA合成。

The purified protein was used to investigate the RNA syntheses from the 3 ′ terminal sequences of HCV positive and negative strand RNA in vitro .

反向遗传技术是一种新的分子生物学技术，它在深入研究负链RNA病毒基因组结构和功能，探寻其基因组复制、转录和研发新型基因工程疫苗上发挥着重要的作用。

Reverse genetics , as a novel technique , has played an important role in study of structure , function of genome of negative-strand RNA virus and exploration of its replication , transcription and development of vaccine .

Lamivudine是一种目前广泛应用的抗病毒药物，它可同时抑制反转录酶和DNA依赖性DNA聚合酶的活性，从而同时阻断HBV负链和正链DNA的合成。

Lamivudine inhibits viral replication at the level of progeny DNA synthesis . Specifically , its incorporation into nascent DNA causes chain termination . This applies both to the reverse transcriptase-and the DNA-dependent DNA polymerase activity of the virus .

这是首次有关负链RNA植物病毒遗传多样性的报道。通过体外转录，将PSTVd负链RNA分别与双价和三价核酶混合，37℃温育2h。

After transcription in vitro , the PSTVd negative RNA was mixed with the ribozyme containing two or three cleavage sites separately and incubated two hours at 37 ℃ .

我们研究27例肝炎病人血清、外周血单个核细胞（PBMCs）及肝脏中GBV-C/HGV正链及负链RNA（复制中间体）的存在状况。

We have studied the presence of plus-and minus-stranded ( replicative intermediate ) GBV-C / HGV RNA in serum , liver , and peripheral blood mononuclear cells ( PBMCs ) from 27 patients .