头孢西丁

χ2检验，头孢西丁纸片扩散法和mecA基因法差异无显著性。

The methods of cefoxitin disk diffusion and mecA gene were not statistically significant differences , by χ ~ 2 test .

结果在MH琼脂使用头孢西丁纸片检测MRSA优于高盐琼脂苯唑西林纸片扩散法，并与PCR检测mecA基因的方法高度一致。

Results Method with cefoxitin disk diffusion in MH disk in detection of MRSA is better than oxacillin salt agar disk ; and it is also highly consistent to the method detecting mecA gene by PCR .

毛细管GC测定头孢西丁钠中残留溶剂

Capillary GC Determination of Residual Organic Solvents in Cefoxitin Sodium

应用头孢西丁三维实验的方法检测细菌产AmpC酶的状况。

AmpC enzyme was examined by Cefoxitin in three dimensional test .

头孢西丁纸片法检测mecA基因介导的MRS

Detection of Meticillin-resistant Staphylococci Mediated by mecA Gene Using Cefoxitin Disk Diffusion Method

方法：应用头孢西丁三维试验检测AmpC酶。

Methods : A cefoxitin three dimensional test was used to detect AmpC enzyme .

用头孢西丁纸片法筛查MRSA阳性82株，阴性63株。

In cefoxitin disk diffusion tests , 82 isolates showed MRSA-positive and 63 isolates were negative .

目的比较头孢西丁纸片扩散法与传统的苯唑西林纸片扩散法对耐甲氧西林葡萄球菌（MRS）检测的效率。

Objective To compare the efficiency between the cefoxitin disk diffusion test and oxacillin disk diffusion test on the detection of methicillin-resistant staphylococcus ( MRS ) .

方法纸片扩散法（K-B法）测定头孢西丁对三代头孢菌素耐药的肠杆菌科细菌的敏感性；

Method The sensitivity of enterobacteriaceae to cefoxitin was determined by K-B method .

SephadexG-10凝胶色谱系统测定头孢西丁钠中高分子聚合物

Determination of HMW Impurities of Cefoxitin Sodium by Sephadex G-10 Chromatographic System

方法用头孢西丁耐药表型筛选阳性可疑菌株，提取耐药质粒，然后采用多重PCR技术检测是否存在质粒介导的AmpC基因及其基因型别；

METHODS Using multiplex PCR technique to amplify AmpC β lactamase gene and differentiate genotypes after extracted plasmid from cefoxitin resistant clinical strains , sequenced PCR products .

结论：21.7%的革兰阴性杆菌能产生IB。用亚胺培南作为诱导剂检测IB的生成，其作用优于头孢西丁。

These results indicate that 21.7 % gram negative bacteria can produce inducible β lactamase and imipenem was more active than cefoxitin as inducer .

方法：采用头孢西丁三维试验检测AmpC酶；NCCLS推荐的纸片扩散确证法检测ESBLs；

Methods : Cefoxitin three-dimensional test and disk diffusion confirmation method recommended by NCCLS were used to detect AmpC enzyme and ESBLs respectively .

结论：头孢西丁K-B法筛选试验结合头孢西丁三相试验可准确检出质粒介导的AmpC酶，可用于临床常规检测。

Conclusion : Cefoxitin screening test combinated with indirect three-dimensional test can detect plasmid-mediated AmpC accurately and can be used in clinical routine detection .

结论头孢西丁耐药的肺炎克雷伯菌大部分菌株同时产两种甚至多种β内酰胺酶，临床微生物实验室应加强对此类菌株所产的β内酰胺酶特别是AmpC酶的监测和研究。

Conclusions These cefoxitin-resistant Klebsiella pneumoniae produced two or more β - lactamases . It is imperative for clinical microbiology laboratories to detect and research β - lactamases , especially AmpC enzyme .

方法采用MicroScanWalkaway40鉴定274株革兰阴性杆菌的药敏情况，并用酶粗提物头孢西丁三维实验检测AmpC酶。

Methods Microscan walkaway-4.0 was used for detecting the susceptibility of antimicrobial agents against gram-negative clinical strains . AmpC enzyme was detected by Cefoxitin in three dimensional test .

应用头孢西丁和苯唑西林纸片扩散法筛查耐甲氧西林的金葡菌（MRSA）；琼脂稀释法检测头孢西丁，苯唑西林和万古霉素的最低抑菌浓度（MIC）。

MRSA was screened by oxacillin and cefoxitin disk diffusion methods . Agar dilution method was used to determine the minimal inhibitory concentrations ( MICs ) of oxacillin , cefoxitin and vancomycin to isolates of staphylococcus aureus .

方法用苯唑西林纸片扩散法、头孢西丁纸片扩散法、mecA基因扩增等方法检测MRSA，用随机扩增多态DNA（RAPD）法对所分离的112株MRSA进行同源性分析。

Methods Oxacillin disk diffusion , cefoxitin disk diffusion test and PCR amplification of mecA gene were used to confirm MRSA strains . A total of 112 MRSA isolates were typed by randomly amplified polymorphic DNA ( RAPD ) .

方法用头孢西丁药敏纸片法（K-B法）和头孢西丁三维试验作AmpC酶的筛选、确证试验，对AmpC酶阳性者进行质粒接合试验。

Methods AmpC enzyme was screened by cefoxitin susceptibility disk test ( K-B method ) and confirmed by cefoxitin three-dimensional test . The strains with AmpC enzyme positive were performed by plasmid conjugation test .

产ESBLs株对亚胺培南均呈敏感，对头孢西丁、头孢哌酮-舒巴坦及哌拉西林-三唑巴坦耐药率较低，对其他抗菌药物的耐药率均较不产ESBLs株高。

All ESBLs producing strains in our study were susceptible to imipenem , and relatively susceptible to cefoxitin , cefoperazone-sulbactam and piperacillin-tazobactam . ESBLs-producers had much higher resistance rates to other antimicrobial agents compared with ESBLs non-producing strains .

头孢西丁纸片法筛选出40株MRSA，其中39株mecA基因阳性，方法特异性为97.5%，灵敏度92.9%；

Forty isolates of MRSA were detected by cefoxitin agar diffusion , in which 39 strains had mecA gene . The specificity and sensitivity of the method was 97.5 % and 92.9 % .

方法：用mecA基因检测MRSA乳胶凝集法、头孢西丁纸片扩散法和苯唑西林微量稀释法对临床分离的160株金黄色葡萄球菌进行检测并作比较。

Methods PCR amplification of the mecA gene , the MRSA-screen latex agglutination test , disk diffusion method with cefoxitin and the microdilution method of oxacillin were performed to analyze 160 clinical isolates of S. aureus .

目的探讨诱导型克林霉素耐药测定的临床价值和评价头孢西丁纸片扩散法检测耐甲氧西林葡萄球菌mecA基因。

OBJECTIVE To investigate the clinical practicability of inducible clindamycin resistance and to evaluate the clinical value of cefoxitin disk diffusion method in detecting the mecA gene of meticillin-resistant Staphylococcus ( MRS ) .

与苯唑西林琼脂平板筛选法比较，头孢西丁纸片扩散法检测MRSA的敏感性和特异性均为100%，而苯唑西林纸片扩散法其敏感性和特异性分别为93%（14/15）和91%（20/22）；

Compared with the results of the oxacillin agar-plate screen test , the percentage of sensitivity ( % ) / specificity ( % ) were as fallows : the cefoxitin disk diffusion test were 100 / 100 on detection of MRSA and MRCNS ;

喹诺酮类抗生素之间存在交叉耐药，同时喹诺酮类抗生素诱导的KPn耐药菌可广泛出现头孢西丁耐药，其共同的耐药机制可能为：外膜蛋白表达减少或缺失。

There is cross-resistance mechanism of KPn to quinolones antibiotics . Induced quinolones-resistant KPn strains were widespread resistance to cefoxitin . Their common mechanism of drug-resistance may be : the reduced or absent expression of outer membrane protein . 4 .

结论头孢西丁纸片扩散试验简便、价廉，不需要特殊的试验条件并能提高测定ORSA常规试验的可靠性，适用于临床实验室对ORSA的常规检测。

CONCLUSIONS The cefoxitin disk diffusion method required no special test conditions would improve the reliability of routine tests for detection of ORSA . Again , It ′ s simple , cheap and suitable for use in clinical laboratories .

头孢西丁与4种止血药配伍的稳定性试验

Experiment on Compatible Stability of Cefoxitin with Four Kinds of Hemostatic

头孢西丁纸片扩散法检测耐甲氧西林葡萄球菌的临床应用评价

Clinical evaluation in detecting methicillin-resistant Staphylococci with cefoxitin disk diffusion method

目的评价注射用头孢西丁钠的安全性。

Objective To evaluate the safety of cefoxitin sodium for injection .

结论头孢西丁筛查试验特异性较差，假阳性高；

Conclusion : The cefoxitin screening test has poor specificity ;