外源基因导入

植物遗传转化是利用重组DNA技术、组织培养技术或种质系统转化等技术，将外源基因导入植物组织或细胞，获得转基因植物。

Transformation of plants is the technology of transferring foreign genes into plants by DNA recombination , tissue culture , or germplasm line .

用PEG法把外源基因导入甘蓝型油菜原生质体再生转基因植株

PEG-mediated gene transfer into Brassica na - pus protoplasts and regeneration of transgenic plants

虽然在实验室条件下人们可以非常精确地对DNA进行剪接，但一个外源基因导入植物体后，对整个植物基因组的影响人们尚不能完全预测和控制。

We can cut DNA exactly in the lab , but we cannot predict and control the effects on the whole plant genes after the " outside " genes which are transplanted into the plant .

利用DNA重组技术将外源基因导入栽培稻创造转基因水稻的方法为提高水稻产量、抗性和品质，解决日益增长的人口和粮食紧缺矛盾以及农业可持续发展开辟了一条崭新的途径。

Recombinant DNA is a new method to create transgenic rice with foreign genes from other species . This method could be helpful to increase rice yield , quality and consequently to solve the problem between population expansion and sustainable development .

[目的]探讨用双嗜性逆转录病毒载体将外源基因导入日本血吸虫（Sj）细胞的生物学理论与实验依据。

[ Objective ] To explore biologically theoretical and experimental foundation for introduction of exogenous gene into Sj cells with amphotropic retrovirus vector .

本研究摸索出了一种高效YAC内基因打靶的新方法，并探索了通过细胞融合的方法将YAC外源基因导入哺乳动物体细胞内的可行性，这为利用YAC进行转基因动物的生产提供了借鉴。

Explored the method of the YAC transfected into goat fibroblast cell by spheroplast fusion and proved the possibility . of this method .

结论：（1）rAAV病毒能将外源基因导入真核细胞，有效和持续地表达，并减少了污染机会。

Conclusion : ( 1 ) rAAV can successfully transfer the foreign gene into the eukaryotic cell , it 's direct genetic alteration should result in continuous , stable expression of foreign gene .

禾谷类作物外源基因导入及表达的研究进展

Research progress of foreign DNA transfer and expression in cereal crops

外源基因导入后水稻粒形的变化一粒沙子

Kernel Morphological Variation after Guiding Exogenous Gene A Grain of Sand

利用He&Ne激光将外源基因导入番茄愈伤组织的研究

Study of Introducing Foreign Gene into Calli of Tomato with the Laser

一种将外源基因导入植物的新方法&激光微束穿刺法

A new way of foreign gene transformation of plant Laser Microbeam Puncture

电脉冲作用将外源基因导入稀有鮈鲫精子的研究

Introduction of Foreign DNA into the Genome of Rare Minnow by Sperm Electroporation

而靶细胞对对外源基因导入可能具有“纠正作用”。

Moreover , target cells may have some'correction mechanism'against the introduced foreign genes .

基因治疗就是将外源基因导入到正常或病变细胞中，利用其转录或翻译产物发挥治疗作用的一种方法，是分子生物学技术在临床医学中的一项重要应用。

Gene therapy plays an important role in clinic coming from molecular biology technique .

外源基因导入对棉花同工酶的影响

Inserted exogenous gene effect on cotton isoenzyme

鱼类精子携带的外源基因导入

Introduction of foreign gene carried by sperms

用两种电激仪将外源基因导入胡萝卜和水稻的比较研究

Comparative Study of Introducing Foreign Gene into Carrot and Rice with Two Kinds of Electroporation System

利用激光微束将外源基因导入高等植物细胞的研究

Study on introduction of the exogenous genes into higher plant cells by using laser microbeam irradition

外源基因导入部分酶解的大麦愈伤组织和转基因植株的再生

Introduction of foreign gene into barley calli after partially enzymatic digestion and regeneration of transgenic plants

要将外源基因导入辣椒，首先必须建立高效的辣椒离体再生体系和稳定的遗传转化体系。

The high efficient system of pepper plant regeneration and genetic transformation must be established before transferring Chitinase and ?

目前外源基因导入玉米受体细胞的方法很多，最受瞩目的是农杆菌介导法和基因枪法。

Agrobacterium and gene gun have aroused most attention among various genetic transformation methods for exogenous gene into maize .

在植物遗传化研究中，外源基因导入受体细胞的方法有多种。

There are many genetic transformation methods for the target gene getting into the receptor cell in plant genetic transformation .

用农杆菌介导法将外源基因导入植物是植物遗传转化的一种首选方法。

It is a good choice of the genetic transformation to introduce foreign genes into plants by Agrobacterium tumefaciens-mediated method .

综述了利用外源基因导入手段进行棉花纤维品质改良的相关研究进展，旨为棉花分子育种研究提供帮助。

The research progress of cotton fiber quality improvement using exogenous genes was summarized to provide help for cotton molecular breeding .

表明通过基因打靶可以将外源基因导入希望的家蚕基因组位点，并实现表达。

These results showed that the heterologous gene could be introduced at the desired locus of the silkworm genome by gene targeting and expressed successfully .

本文介绍了外源基因导入植物的各种新技术的基本原理、操作要点及其在基因转移工作中所取得的成就。

The basic principles and the manipulating key of various techniques for transfering foreign genes into plant and the achievements of transgenic techniques in plant had been introduced .

笔者综述了近年来外源基因导入小麦的研究概况，阐述了外源基因导入的技术以及检测方法，分析了外源基因转化在小麦育种中的发展前景。

This report summarized the researching status of wheat exogenous gene transformation in recent years . The prospects of its applications in wheat breeding field was also analysed .

这一实验体系正在用于抗菌核病的诱变与筛选，并作为外源基因导入的实验体系。

The experiment system developed by us for rape , is being used for induction and screening of sclerotinia sclerotiorum resistant mutant and introduction of foreign gene into rape .

探讨家蚕精子介导基因转移技术能否有效地将外源基因导入并整合到家蚕基因组中，使之稳定遗传。

In this study , the effiency of sperm-mediated gene transfer was analyzed for foreign gene introduced into and intergrated in the silkworm ′ s genome and inherited by the offspring .

研究表明用基因枪法转化处理棉花幼胚是一种操作简便、重复性好的转化方法，用该法可将外源基因导入幼胚，因而避开了植株离体再生的困难。

The results indicated that the method was easy to operate and repetitive . The foreign genes were introduced into immature embryos by this technique , which avoids difficulties of regeneration of plantlets .